Anju Mohan and Hari Mohan Saxena*
Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, India
*Corresponding Author: Hari Mohan Saxena, Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, India.
Received: June 15, 2020; Published: August 26, 2020
Brucellosis caused by Brucella organisms is a major zoonosis globally. It causes heavy losses through abortions, delayed conception and infertility in animals. Antibiotic therapy is ineffective. Once infected, the animal remains carrier and sheds bacteria in milk, semen and uterine discharges for long period. We have successfully used a lytic brucella phage for therapy of Brucellosis in adult cattle. We also targeted the phage employing the live attenuated Brucella abortus strain 19 organisms to kill the virulent Brucella residing intracellularly in phagocytes in the body. The effect of both the therapies was monitored non-invasively employing Brucella RNA in blood plasma as a Brucella - specific biomarker. A single dose of the phage alone or the therapeutic vaccine (phage pulsed S-19) could substantially reduce and finally eliminate live Brucella in the body within 3 months as evident from diminished and ultimately non – detectable RNA characteristic of Brucella abortus (223 bp amplicon) in plasma by RT-PCR. Thus, phage has a potential to cure Brucellosis and abolish carrier state in cattle and RNA can serve as a specific biomarker of live Brucella for monitoring and assessment of the efficacy of the therapy.
Keywords: Therapeutic Vaccine; Brucellosis; Brucella; Brucella Phage; Phage Therapy; Phage Targeting
Citation: Anju Mohan and Hari Mohan Saxena. “Non - Invasive Monitoring and Assessment of Phage Therapy and Phage Targeting in Bovine Brucellosis Employing a Brucella - Specific Biomarker". Acta Scientific Microbiology 3.9 (2020): 59-68.
Copyright: © 2020 Anju Mohan and Hari Mohan Saxena. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.