Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 9

Diversity of Begomoviruses Infecting Okra [Abelmoschus esculentus (L.) Moench] in Togo

Fidèle Tiendrébéogo1,2*, Ayékitan Akakpo1,2,3, Ezechiel B Tibiri1,2, Monique Soro1,2, Cheikna Zongo3, Aly Savadogo3, James Bouma Neya1,2, Kossikouma Djodji Adjata4 and Nicolas Barro5

1Laboratoire de Virologie et de Biotechnologies Végétales, INERA/CNRST, Burkina Faso
2Laboratoire Mixte International Patho-Bios, IRD-INERA, Burkina Faso
3Laboratoire de Biotechnologie et d’Immunologie Appliquée (LaBIA), Université Joseph Ki-Zerbo, Burkina Faso
4Laboratoire de Virologie de Biotechnologies Végétales, Ecole Supérieure d’Agronomie, Université de Lomé, Togo
5Laboratoire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmissibles par les Aliments et l’eau, LabESTA/UFR/SVT, Université Joseph Ki-Zerbo, Burkina Faso

*Corresponding Author: Fidèle Tiendrébéogo, Laboratoire de Virologie et de Biotechnologies Végétales, Institut de l’Environnement et de Recherches Agricoles (INERA), Burkina Faso.

Received: July 29, 2020; Published: August 18, 2020

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Abstract

Okra [Abelmoschus esculentus (L.) Moench] is an important crop which is widely grown in Africa. It is a major source of fiber and vitamins for diet and incomes for several women farmers. The crop is negatively impacted by okra leaf curl disease (OLCD) which is an endemic disease in Africa and is caused by a complex of begomoviruses. To determine the identity and diversity of the virus species responsible for OLCD in Togo, Okra fields were assessed and 118 okra leaves were sampled and analyzed using polymerase chain reaction (PCR) with specific primers following sequencing. During fields’ surveys, OLCD prevalence has been estimated at 64% in Dry Savannah zone, 60% in Forest zone, 53% in Littoral zone and 50% in Humid Savannah zone. Altogether, the average prevalence of the disease in Togo is about 57%. Using, PCR to diagnose the presence of begomoviruses in the samples collected, ~56% were positive. Among the begomoviruses positive samples, ~38% were found associated with betasatellite molecules. The Coat Protein gene sequencing has revealed the presence of Cotton leaf curl Gezira virus (CLCuGeV) and Okra yellow crinkle virus (OYCrV) in Togo. This study highlights the complexity of begomoviruses associated to OLCD in Togo.

Keywords: Okra; Leaf Curl Disease; PCR; Coat Protein; Begomovirus; Togo

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References

  1. Alegbejo M., et al. “Survey for incidence of Okra mosaic virus in northern Nigeria and evidence for its transmission by beetle”. Spanish Journal of Agricultural Research 6 (2008): 408-411.
  2. Food and Agriculture Data (2018).
  3. Kumar S., et al. “Okra (Abelmoschus spp.) in West and Central Africa: Potential and progress on its improvement”. African Journal of Agricultural Research25 (2010): 3590-3598.
  4. Brunt A., et al. “Viruses of Tropical Plants”. CAB International, Wallingford, UK (1990).
  5. Swanson MM and Harrison BD. “Serological relationships and epitope profiles of isolates of okra leafcurl geminivirus from Africa and the Middle-East”. Biochimie 75 (1993): 707-711.
  6. Ndunguru J and Rajabu AC. “Effect of okra mosaic virus disease on the above- ground morphological yield components of okra in Tanzania”. Scientia Horticulturae 99 (2004): 225-235.
  7. Shih SL., et al. “Complete nucleotide sequences of okra isolates of Cotton leaf curl Gezira virus and their associated DNA-b from Niger”. Archives of Virology 154 (2009): 369-372.
  8. Idris AM and Brown JK. “Molecular Analysis of Cotton Leaf Curl Virus-Sudan Reveals an evolutionary history of recombination”. Virus Genes 24 (2002): 249-256.
  9. Kon T., et al. “Roles and interactions of begomoviruses and satellite DNAs associated with okra leaf curl disease in Mali, West Africa”. Journal of General Virology 90 (2009): 1001-1013.
  10. Tiendrébéogo F., et al. “Impact of okra leaf curl disease on morphology and yield of okra”. Crop Protection 29 (2010): 712-716.
  11. Shih SL., et al. “First report of a distinct begomovirus associated with okra yellow crinkle disease in Mali”. Plant Pathology 56 (2007): 718.
  12. Bigarré L., et al. “Characterization of a new begomovirus from Egypt infecting hollyhock (Althearosea)”. European Journal of Plant Pathology 107 (2001): 701-711.
  13. Idris AM., et al. “Phylogenetic relationships for okra leaf curl- and hollyhock leaf crumple-associated begomoviruses and first report of associated satellite DNAs”. Arab Journal of Biothechnology 5 (2002): 67-82.
  14. Tiendrébéogo F., et al. “Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso”. Virology Journal 7 (2010): 48.
  15. Zerbini FM., et al. “ICTV virus taxonomy profile: Geminiviridae”. Journal of General Virology 98 (2017): 131-133.
  16. Briddon RW., et al. “Alphasatellitidae: a new family with two subfamilies for the classification of geminivirus- and nanovirus-associated alphasatellites”. Archives of Virology 163 (2018): 2587-600.
  17. Mansoor S., et al. “Identification of a novel circular single stranded DNA associated with cotton leaf curl disease in Pakistan”. Virology 259 (1999): 190-199.
  18. Briddon RW. and Stanley J. “Sub-viral agents associated with plant-infecting single-stranded DNA viruses”. Virology 344 (2006): 198-210.
  19. Saunders K., et al. “Replication promiscuity of DNAb satellites associated with monopartite begomoviruses: deletion mutagenesis of the Ageratum yellow vein virus DNAb satellite localises sequences involved in replication”. Journal of General Virology 89 (2008): 3165-3172.
  20. Leke WN., et al. “Begomovirus disease complex: emerging threat to vegetable production systems of West and Central Africa”. Agriculture and Food Security 4 (2015): 1
  21. Permingeat HR., et al. “A simple method for isolating DNA of high yield and quality from cotton (shape Gossypiumhirsutum L.) leaves”. Plant Molecular Biology Reporter 16 (1998): 89-89.
  22. Delatte H., et al. “South West Indian Ocean island stomato begomovirus populations represent a new major monopartite begomovirus group”. Journal of General Virology 86 (2005): 1533-1542.
  23. Briddon RW., et al. “Universal primers for the PCR-mediated amplification of DNA b: a molecule associated with some monopartite begomoviruses”. Molecular Biotechnology 20 (2002): 315-318.
  24. Kumar S., et al. “MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets”. Molecular Biology and Evolution 33 (2016): 1870-1874.
  25. Séka K., et al. "First reports of cotton leaf curl Gezira virus and okra yellow crinkle virus associated with okra leaf curl disease in Côte d’Ivoire". New Disease Reports 34 (2016). 1.
  26. Ouattara A., et al. "Diversity, distribution and prevalence of vegetable-infecting geminiviruses in Burkina Faso". Plant Pathology2 (2020): 379-392.
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Citation

Citation: Fidèle Tiendrébéogo., et al. “Diversity of Begomoviruses Infecting Okra [Abelmoschus esculentus (L.) Moench] in Togo". Acta Scientific Microbiology 3.9 (2020): 29-33.




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