Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Commentary Volume 3 Issue 9

Traffic Light Reporter for Genome Engineering

Kawalpreet K Aneja*

Department of Molecular Biology, University of Pennsylvania, Ambler, Pennsylvania, USA

*Corresponding Author: Kawalpreet K Aneja, Department of Molecular Biology, University of Pennsylvania, Ambler, Pennsylvania, USA.

Received: July 27, 2020; Published: August 18, 2020

×

Gene editing depends upon the cell’s endogenous DNA repair pathways. Upon DNA double-strand break, two known repair pathways are enacted: nonhomologous end-joining (NHEJ) and homologous recombination (HDR), among others. The HDR pathway works only in the presence of an exogenous homologous template. Is it possible to determine which pathway is activated in a cell after gene disruption (GD)? Is it NHEJ or HDR? Certo., et al. 2011 [1] published an article on the Traffic Light Reporter (TLR) that can distinguish between NHEJ and HR events. Traffic light reporter system is designed such that cells generate flow-cytometric readout of HDR-mediated gene targeting or mutagenic NHEJ-mediated gene disruption occurring at an individual DNA breakpoint, which is extremely exciting!

×

Citation

Citation: Kawalpreet K Aneja. “Traffic Light Reporter for Genome Engineering". Acta Scientific Microbiology 3.9 (2020): 27-28.



Indexed In






News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is December 20, 2020.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of “Best Article of the Issue”.
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.
  • Contact US