Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Commentary Volume 3 Issue 9

Traffic Light Reporter for Genome Engineering

Kawalpreet K Aneja*

Department of Molecular Biology, University of Pennsylvania, Ambler, Pennsylvania, USA

*Corresponding Author: Kawalpreet K Aneja, Department of Molecular Biology, University of Pennsylvania, Ambler, Pennsylvania, USA.

Received: July 27, 2020; Published: August 18, 2020

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Gene editing depends upon the cell’s endogenous DNA repair pathways. Upon DNA double-strand break, two known repair pathways are enacted: nonhomologous end-joining (NHEJ) and homologous recombination (HDR), among others. The HDR pathway works only in the presence of an exogenous homologous template. Is it possible to determine which pathway is activated in a cell after gene disruption (GD)? Is it NHEJ or HDR? Certo., et al. 2011 [1] published an article on the Traffic Light Reporter (TLR) that can distinguish between NHEJ and HR events. Traffic light reporter system is designed such that cells generate flow-cytometric readout of HDR-mediated gene targeting or mutagenic NHEJ-mediated gene disruption occurring at an individual DNA breakpoint, which is extremely exciting!

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Citation

Citation: Kawalpreet K Aneja. “Traffic Light Reporter for Genome Engineering". Acta Scientific Microbiology 3.9 (2020): 27-28.




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Acceptance rate30%
Acceptance to publication20-30 days

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