Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Case Report Volume 3 Issue 8

A Simulation Study on the Effects of Media Composition on the Growth Rate of Escherichia coli MG1655 Using iAF1260 Model

Kar Chi Cheong1, Raphael YH Hon1, Clara J Sander1, Irwin ZL Ang1, Jun Hang Foong1 and Maurice HT Ling1,2*

1School of Applied Sciences, Temasek Polytechnic, Singapore
2HOHY PTE LTD, Singapore

*Corresponding Author: Maurice HT Ling, School of Applied Sciences, Temasek Polytechnic and HOHY PTE LTD, Singapore.

Received: June 22, 2020; Published: July 22, 2020

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Abstract

Media compositions are important determinants of growth rate and genome-scale models (GSMs) had been used for optimizing media for metabolite production and growth. Recently, iAF1260, a GSM based on Escherichia coli MG1655, was used to study the effects varying glucose concentration in media on growth rate and metabolic fluxes. In this study, the effects of other media components in the presence of varying glucose concentrations on the predicted growth rate of E. coli MG1655 were examined. Our results show that 10 media components (ammonium, calcium, chloride, copper, glucose, manganese, magnesium, molybdate, phosphate, and potassium) demonstrate substantial impact on the predicted growth rate of E. coli MG1655. Of which, 4 components (glucose, ammonium, magnesium, and phosphate) have the most impact. However, our results also demonstrate the limitations of iAF1260 as media components that had been shown to affect E. coli growth rate were not reflected by the model.

Keywords: Growth Rate; Genome-Scale Models; Media Optimization

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Citation

Citation: Maurice HT Ling., et al. “A Simulation Study on the Effects of Media Composition on the Growth Rate of Escherichia coli MG1655 Using iAF1260 Model". Acta Scientific Microbiology 3.8 (2020): 40-44.



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