Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 7

Isolation, Identification and Characterization of Microorganisms Isolated from Unexplored Saline Regions of Kutch, Gujarat, India

Josh CM1* and Trivedi NS2

1C U Shah University, Wadhwan City, Gujarat, India
2Gujarat University, Ahmadabad, Gujarat, India

*Corresponding Author: Josh CM, C U Shah University, Wadhwan City, Gujarat, India.

Received: April 11, 2020; Published: June 09, 2020

×

Abstract

  The present investigation reveals the diversity of bacteria, fungus and archaea along the different regions of Kutch area, Gujarat, India. The samples were collected from 3 selected site of Kutch area whose temperature was between 37 - 41°C, pH 8.5, EC 1480 μs, Salinity 9.01 and 1.85 ppm TDS. 52 bacterial, 17 extreme halophilic archaea and 2 halophilic fungus strains were isolated, among these all bacterial strains were selected for further study like enzyme productivity and anti-microbial activity because the main aim of research was to isolate organism which have multiple applications. Fifteen strains produced four or more than four enzymes which have industrial application (amylase, protease, cellulose, lipase, and chitinase) and one strain of Micrococcus showed antimicrobial activity against all test cultures. Eighteen archaea and two halophilic fungi that grew at 25% salt containing medium were also isolated.

Keywords: The present investigation reveals the diversity of bacteria, fungus and archaea along the different regions of Kutch area, Gujarat, India. The samples were collected from 3 selected site of Kutch area whose temperature was between 37 - 41°C, pH 8.5, EC 1480 μs, Salinity 9.01 and 1.85 ppm TDS. 52 bacterial, 17 extreme halophilic archaea and 2 halophilic fungus strains were isolated, among these all bacterial strains were selected for further study like enzyme productivity and anti-microbial activity because the main aim of research was to isolate organism which have multiple applications. Fifteen strains produced four or more than four enzymes which have industrial application (amylase, protease, cellulose, lipase, and chitinase) and one strain of Micrococcus showed antimicrobial activity against all test cultures. Eighteen archaea and two halophilic fungi that grew at 25% salt containing medium were also isolated.

×

References

  1. Ventosa A. “Unusual microorganisms from unusual habitats: hypersaline environments”. In: Logan NA, Lappin-Scott HM, Oyston PCF, editors. Prokaryotic diversity-mechanism and significance. Cambridge University Press; Cambridge (2006): 223-253.
  2. M Bagheri., et al. “Bacillus iranensis nov., a moderate halophile from a hypersaline lake”. International Journal of Systematic and Evolutionary Microbiology 62 (2012): 811-816.
  3. Jose PA and Jebakumar SRD. “Phylogenetic diversity of actinomycetes cultured from coastal multipond solar saltern in Tuticorin, India”. Aquatic Biosystems (2012): 8.
  4. BP Dave and A Soni. “Diversity of halophilic archaea at salt pans around Bhavnagar coast, Gujarat”. Proceedings of the National Academy of Sciences India Section B: Biological Sciences2 (2013): 225-232.
  5. JU Vora and HA Modi. “Bacterial diversity of soil samples of saline sites in Kharaghoda (Tehsil-Patdi, District-Surendranagar), Gujarat”. International Journal of Research in Pure and Applied Microbiology 3 (2013): 102-106.
  6. Pravin R., et al. “Microbial Biodiversity Exploration for Liquid biofertilizer Application from Wet Land of Little of Little Rann of Kutch, Gujarat, India”. Proceedings of Taal (2012).
  7. Pal KK., et al. “Draft genome sequence of Bacillus sp. strain SB47, an obligate extreme halophile isolated from a salt pan of the Little Rann of Kutch, India”. Genome Announcement5 (2013): e00816-e00813.
  8. Manesh Thomas., et al. “A novel haloarchaeal lineage widely distributed in the hypersaline marshy environment of Little and Great Rann of Kutch in India”. Current Science9 (2012): 1078-1084.
  9. Thumar JT., et al. “Isolation and partial purification of an antimicrobial agent from halotolerantalkaliphilic Streptomyces aburaviensis strain Kut-8”. World Journal of Microbiology and Biotechnology 26 (2010): 2081-2087.
  10. Kutch AS Pandit., et al. “Metagenomes from the Saline Desert of Gujarat State Biotechnology Mission”. Genome Announcements3 (2014).
  11. Vivek Upasani and Suresh Desai. “Sambhar Salt Lake Chemical composition of the brines and studies on haloalkaliphilic archaebacteria”. Archives of Microbiology 154 (1990): 589-593.
  12. Kamal K Pal., et al. “Draft Genome Sequence of an Extreme Haloarchaeon 3A1-DGR Isolated from a Saltern Crystallizer of the Little Rann of Kutch, India”. Indian Journal of Microbiology 54 (2014): 471-473.
  13. Sara Cuadros-Orellanaa., et al. “Biodegradation of Aromatic Compounds by a Halophilic Archaeon Isolated from the Dead Sea”. 3rd International Conference on Industrial Biotechnology 27 (2012).
  14. Younes Ghasemia., et al. “Screening and Isolation of Extracellular Protease Producing Bacteria from the Maharloo Salt Lake”. Iranian Journal of Pharmaceutical Sciences 3 (2011).
  15. K Asha., et al. “Isolation and Cultivation of Halophilic Archaea from Solar Salterns Located in Peninsular Coast of India”. The Internet Journal of Microbiology2 (2004).
  16. Hassanshahian and Jafar Mohamadian. “Isolation and characterization of Halobacterium salinarum from saline lakes in Iran Mehdi”. Jundishapur Journal of Microbiology (2011).
  17. Thabet Ibrahim Qassem. “Isolation of Halophilic Microorganisms From salted soil in Jazan area”. Biology Department -Faculty of Science- Jazan University.
  18. G Ellis., et al. “Halophilic Archaea determined from geothermal steam vent aerosols”. Environmental Microbiology6 (2008): 1582-1590.
  19. Stephen Kugbere Agadagba. “Isolation of Actinomycetes from Soil”. Journal of Microbiology Research3 (2014): 136-140.
  20. Amit Pandey., et al. “Isolation and Characterization of Actinomycetes From Soil and Evaluation of Antibacterial Activities of Actinomycetes Against Pathogens” International Journal of Applied Biology and Pharmaceutical Technology1 (2011).
  21. Anuprita A Deshmukh and Narendra N Vidhale. “Characterization of Novel Antibacterial Actinomycetes Strain N8 from Saline Soil of Vidarbha Region”. International Journal of Life Sciences Biotechnology and Pharma Research1 (2015).
  22. Department of microbiology, govt. Rajaram college, vidyanagar, Kolhapur, MS, INDIA “Biodiversity of Actinomycetes in Hypersaline soils of Kolhapur district and their screening as potential antibiotic producer, India”. Research Journal of Recent Sciences (2012): 317-319.
  23. Zuckerkandi E and Pauling L. “Molecules as documents of evolutionary history”. Journal of Theoretical Biology2 (1965): 357-366.
  24. Tortoli E. “Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990s”. Clinical Microbiology Reviews2 (2003): 319-354.
  25. Pfister P., et al. “Role of 16s rRNA helix 44 in ribosomal resistance to hygromycin B”. Antimicrobial Agents and Chemotherapy5 (2003): 1496-1502.
  26. Woese CR and Fox GE. “Phylogenetic structure of the prokaryotic domain: the primary kingdoms”. Proceedings of the National Academy of Sciences11 (1977): 5088-5090.
  27. Paula Monteiro de Souza and Pérola de Oliveira Magalhães. “Application of microbial α-amylase in industry – A review”. Journal of Microbiology 4 (2010): 850-861.
  28. Houde A., et al. “Lipases and their industrial applications: an overview”. Applied Biochemistry and Biotechnology1-3 (2004): 155-170.
×

Citation

Citation: Josh CM and Trivedi NS. “Isolation, Identification and Characterization of Microorganisms Isolated from Unexplored Saline Regions of Kutch, Gujarat, India". Acta Scientific Microbiology 3.7 (2020): 03-09.



Member In






News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is July 15, 2020.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of “Best Article of the Issue”.
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.
  • Contact US