Abstract

  Genetic engineering has proven to be an efficient strategy for the development of Disease resistant crops. Gram negative bacteria Ralstonia solanacearum, the causative agent of wilt has been causing devastating economic losses in tomato crop yield worldwide. In this study Cocculus hirsutus trypsin inhibitor has demonstrated for its bactericidal activity. Transgenic plants expressing ChTI were developed conferring resistance to Gram negative bacteria, particularly R. solanacearum. In vitro and In vivo studies revealed the bactericidal activity of ChTI with mortality rate upto 42% and 48% in E. coli and R. solanacearum at 500 TIU/mg respectively. Transgenic plants expressing ChTI also developed resistance to the wilt. Whereas non transgenic plants developed symptoms of the disease. Further Strong interaction patterns of ChTI-Lipid and ChTI-membrane proteins revealed the possible mechanism of action of these PIs. These results demonstrate the effectiveness of ChTI as bactericidal agent and is able to enhance resistance in transgenic plants against bacterial pathogens.

Keywords: R. Solanacearum; Cell Membrane Binding Protein; Cocculus hirsutus Trypsin Inhibitor; Evans Blue Assay; Spot Dilution Bacterial Assay; In Silico Protein-Lipid Interactions

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Citation

Citation: Theertha Prasad D., et al. “Cocculus hirsutus Trypsin Inhibitor Confers Resistance to Ralstonia solanacearum: In silico Analysis". Acta Scientific Microbiology 3.6 (2020): 63-68.

Copyright

Copyright: © 2020 Theertha Prasad D., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.