Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 6

Cocculus hirsutus Trypsin Inhibitor Confers Resistance to Ralstonia solanacearum: In silico Analysis

Manushree V1,2, Udit NM1,3, Manjunath BJ1, Devaraj VR 2 and Theertha Prasad D1*

1Department of Plant Biotechnology, University of Agricultural Sciences, GKVK, Bangalore, India
2Department of Biochemistry, Bangalore University, Central College Campus, Bangalore, India
3Department of Biochemistry and Crop physiology, MS Swaminathan School of Agriculture, Centurion University of Technology and management, Paralakhemundi, Odisha, India.

*Corresponding Author: D Theertha Prasad, Professor, Department of Plant Biotechnology, University of Agricultural Sciences, GKVK, Bangalore, India.

Received: April 08, 2020; Published: May 13, 2020

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Abstract

  Genetic engineering has proven to be an efficient strategy for the development of Disease resistant crops. Gram negative bacteria Ralstonia solanacearum, the causative agent of wilt has been causing devastating economic losses in tomato crop yield worldwide. In this study Cocculus hirsutus trypsin inhibitor has demonstrated for its bactericidal activity. Transgenic plants expressing ChTI were developed conferring resistance to Gram negative bacteria, particularly R. solanacearum. In vitro and In vivo studies revealed the bactericidal activity of ChTI with mortality rate upto 42% and 48% in E. coli and R. solanacearum at 500 TIU/mg respectively. Transgenic plants expressing ChTI also developed resistance to the wilt. Whereas non transgenic plants developed symptoms of the disease. Further Strong interaction patterns of ChTI-Lipid and ChTI-membrane proteins revealed the possible mechanism of action of these PIs. These results demonstrate the effectiveness of ChTI as bactericidal agent and is able to enhance resistance in transgenic plants against bacterial pathogens.

Keywords: R. Solanacearum; Cell Membrane Binding Protein; Cocculus hirsutus Trypsin Inhibitor; Evans Blue Assay; Spot Dilution Bacterial Assay; In Silico Protein-Lipid Interactions

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Citation

Citation: Theertha Prasad D., et al. “Cocculus hirsutus Trypsin Inhibitor Confers Resistance to Ralstonia solanacearum: In silico Analysis". Acta Scientific Microbiology 3.6 (2020): 63-68.



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