Abstract

  Organisms were isolated and cultured from two marine fishes (Stolephorus indicus and Nemipterus japonicus) that were exhibiting Bioluminescence. Identification and assessment of impact of five different culture media on the growth and light emission properties were studied. Isolates from both the fishes showed microbes to be gram negative Coccobacilli, Partial gene sequencing analysis suggested that the microbes belonged to Vibrio spp and Photobacterium spp. Biochemical analysis of both the bacterial isolates exhibited difference in sugar (Mannitol and Lactose), LDC, Indole and Urea content; suggesting that the two isolated colonies are different bioluminescent bacteria. Repeated subculture (to obtain pure colonies) of both the isolates resulted in gradual reduction in the intensity of luminescence of the bacteria and eventually loss of luminescence property occurred. However, luminescence was revived when they were grown in aerated condition.

Keywords: Bioluminescence; Anchovy Fish; Threadfin Bream Fish; Growth Curve; Lux Operon; Luciferase; Boss Medium

References

1. Bluth BJ., et al. “Cell-Cell Communication and the lux operon in Vibrio fischeri”, Pittsburgh: Department of Biological Sciences. Carnegie Mellon University (2003).
2. Coval K., et al. “Molecular Biology of Prokaryotes Term Paper Subjects and Teams: Quorum Sensing: Table of Contents” (1999).
3. B Danyluk., et al. “BilskaAn objective Method to Assess Bioluminescent Properties of Selected Bacterial Strains”. Acta Scientiarum Polonorum Technologia (2007): 5-16.
4. T Gokhale., et al. “Research of Marine Isolates in Development of Biosensor for environmental Pollutants”. Engineering Review1 (2012): 17-22.
5. MW Griffith. “Applications of Bioluminescence in the Dairy Industry”. Journal of Dairy Science10 (1993): 3118-3125.
6. M Kannahi and S Sivasankari. “Isolation and Identification of Bioluminescent Bacteria from Marine Water at Nagapattinam Sea Shore Area”. International Journal of Pharmaceutical Science Review and Research 59 (2014): 346-351.
7. K Kita-Tsukamoto., et al. “Rapid identification of marine bioluminescent bacteria by amplified 16S ribosomal RNA gene restriction analysis”. FEMS Microbiology Letters 256 (2006): 298-303.
8. AR Kumar. “Isolation of Luminescent Bacteria from Bay of Bengal and their Molecular Characterization”, University of Borås/School of Engineering (2010).
9. LC Lin and EA Meighen. Bacterial Bioluminescence (2009).
10. J Malave-Orengo., et al. “Isolation and characterization of bioluminescent bacteria from marine environments of Puerto Rico, Current Research, Technology and education Topics in Applied”. Microbiology and Microbial Biotechnology (2010): 103-108.
11. EA Meighen and EA Bacterial “Bioluminescence: organization, regulation and application of the lux genes”. FASEB Journal 7 (1993): 1016-1022.
12. EA Meighen. “Molecular biology of bacterial bioluminescence”. Microbiology Reviews 1 (1991): 123.
13. A Nawaz and N Ahmed. “Isolation and Characterization of Indigenous Luminescent Marine Bacteria from Karachi Coast”. Academic Research International2 (2011): 74-83.
14. VDS Nunes-Halldorson and NL Duran. “Bioluminescent Bacteria: Lux genes as an environmental biosensors”. Brazilian Journal of Microbiology 34 (2003): 91-96.
15. FL Thompson., et al. “The Biology of Vibrios”, Washington, ASM Press (2006): 204-218.
16. Warner JA and JF Case. “The zoogeography and dietary induction of bioluminescence in the midshipman fish, Porichthys notatus”. The Biological Bulletin 159 (1980): 231-246.
17. Haddock HD. "Bioluminescence in the Sea”. Annual Review of Marine Science (2010): 443-493.
18. Labas YA., et al. “On the origin of bioluminescent systems”. In Bioluminescence and Chemiluminescence 2000, edition. JF Case, PJ Herring, BH Robison, SHD Haddock, LJ Kricka, PE Stanley (2000): 91-94.
19. Yamaguchi S and Endo K. “Molecular phylogeny of Ostracoda (Crustacea) inferred from 18S ribosomal DNA sequences: implication for its origin and diversification”. Marine Biology 143 (2003): 23-38.
20. Tinn O and Oakley T. “Erratic rates of molecular evolution and incongruence of fossil and molecular divergence time estimates in Ostracoda (Crustacea)”. Molecular Phylogenetics and Evolution 48 (2008): 157-167.
21. Forey PL and Patterson C. “Description and systematic relationships of Tomognathus, an enigmatic fish from the English Chalk”. Journal of Systematic Palaeontology 4 (2006): 157-184.
22. Carnevale G. “Miniature deep-sea hatchetfish (Teleostei: Stomiiformes) from the Miocene of Italy”. Geological Magazine 145 (2008): 73.
23. Latz M and Rohr J. “Luminescent response of the red tide dinoflagellate Lingulodinium polyedrum to laminar and turbulent flow”. Limnology and Oceanography 44 (1999): 1423-435.
24. Chen AK., et al. “Evidence for the role of G- proteins in flow stimulation of dinoflagellate bioluminescence”. American Journal of Physiology Regulatory, Integrative and Comparative Physiology 292 (2007): R2020-R2027.
25. Dassow Peter von., et al. “Bioluminescent response of the dinoflagellate Lingulodinium polyedrum to developing flow: Tuning of sensitivity and the role of desensitization in controlling a defensive behavior of a planktonic cell”. Limnology and Oceanography 2 (2005): 607-619.
26. Latz M., et al. “Bioluminescent response of individual dinoflagellate cells to hydrodynamic stress measured with millisecond resolution in a microfluidic device”. The Journal of Experimental Biology 211 (2008): 2865-2875.
27. Panceri M. “Etudes sur la phosphorescence des animaux marins”. Annales des Sciences Naturelles 16 (1872): 1-67.
28. Mackie GO. “Propagation of bioluminescence in Euphysa japonica hydromedusae, (Tubulariidae)”. Hydrobiologia 216 (1991): 581-588.
29. Daunert S and Deo SK. “Photoproteins in Bioanalysis”. New York: Wiley-VCH (2006): 256.
30. Bode VC., et al. “Daily rhythm of luciferin activity in Gonyaulax polyedra”. Science 141 (1963): 913-915.
31. Lapointe M and Morse D. “Reassessing the role of a 3′-UTR-binding translational inhibitor in regulation of circadian bioluminescence rhythm in the dinoflagellate Gonyaulax”. Journal of Biological Chemistry 389 (2008): 13-19.

Citation

Citation: Anuradha Pandey and Madhuri Sharon. “Culture Parameters Affect the Light Emitting Property of Organisms Isolated from Two Marine Fishes". Acta Scientific Microbiology 3.5 (2020): 55-60.

Copyright

Copyright: © 2020 Anuradha Pandey and Madhuri Sharon. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.