Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 5

Extraction and Preliminary Characterization of Potential Antibacterial Agent from Potential Streptomyces cinereoruber sp. Isolated from an Chilika Lake

Anjani K Upadhyay1, Debasmita Chatterjee1, Madhuri Swain1 and Lopamudra Ray1,2*

1School of Biotechnology, KIIT University, Bhubaneswar, Odisha, India
2School of Law, KIIT University, Bhubaneswar, Odisha, India

*Corresponding Author: Lopamudra Ray, Assistant Professor, School of Law, Campus-16, Adjunct Faculty, School of Biotech, Campus-11, Kalinga Institute of Industrial Technology (KIIT), Bhubaneswar, Odisha, India.

Received: March 03, 2020; Published: April 13, 2020



  Streptomyces, isolated from marine and estuarine habitat have been widely recognized as a potential source of antifungal, anti-tumour, anti-bacterial compounds. In the present study, the antimicrobial agent production potential of a Streptomyces cinereoruber sp was evaluated. The selective isolation of the strain was carried out on starch casein agar. The primary screening of the Streptomyces isolate was done by cross streak method against pathogenic test strains Escherichia coli MTCC 82, Staphylococcus aureus MTCC 96, Bacillus cereus IP406 and Salmonella typhi MTCC 734 and Micrococcus luteus and the antimicrobial property against Micrococcus luteus was confirmed. The secondary screening was carried out by using the culture supernatant against the test strain by agar well diffusion method. The growth and antimicrobial production ability of the strain against Micrococcus luteus was studied. The antimicrobial agent production was also observed till pH 11 and NaCl concentration 3% (w/v). The partially purified compound showed a peak similar to streptomycin in HPLC. The culture condition for the production of the compound was opimised.

Keywords: Streptomyces cinereoruber; Escherichia coli; Staphylococcus aureus; Micrococcus luteus



  1. Taddi A., et al. “Isolation and identification of streptomyces sp. From Venezuelan site: Morphological and biochemical studies”. Microbiological Research 161 (2006): 222-231.
  2. Jayapal KP., et al. “Comparative genomic hybridizations reveal absence of large Streptomyces coelicolor genomic islands in Streptomyces lividans”. BMC Genomics 8 (2007): 229.
  3. Schone R. “An antibiotic which inhibits Corynebacterium diphtheriae produced by S form of Streptomyces thermophiles”. Antibiotics and Chemotherapy 1 (1951): 176-180.
  4. Sanghvi GV., et al. “Isolation and partial purification of erythromycin from alkaliphilic Streptomyces werraenans isolated from Rajkot, India”. Biotechnology Report (2014): 2-7.
  5. Wang C., et al. “Antifungal activity of volatile organic compounds from Streptomyces alboflavus TD-1”. FEMS Microbiology Letters 341 (2013): 45-51.
  6. Holder IA and Boyce ST. “Agar well diffusion assay testing of bacterial susceptibility to various antimicrobials in concentration non toxic for human cell in culture”. Burns5 (1994): 426-429.
  7. Shirling EB and Gottlieb D. “Methods for characterization of Str8eptomyces species”. International Journal of Systematic and Evolutionary Microbiology 16 (1966): 313-340.
  8. Kelly KI. “Inter-society colour council-national bureau of standard color-name charts illustrated with centroid colors”. US Government Printing Office, Washington (1964).
  9. Gordon RE., et al. “Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain”. International Journal of Bacteriology 24 (1974): 54-63.
  10. Williams ST., et al. “Numerical classification of Streptomyces and related genera”. Journal of General Microbiology 129 (1983): 1743-1813.
  11. Goodfellow M., et al. “The Streptomyces violaceus niger clade: a home for streptomycetes with rugose ornamented spores”. Antonie Van Leeuwenhoek 92 (2007): 173-199.
  12. Kampfer P and Labeda DP. “International Committee on Systematics of Prokaryotes; Subcommittee on the taxonomy of the Streptomycetaceae: Minutes of the meeting, 25 July 2005, San Francisco, CA, USA”. International Journal of Systematic and Evolutionary Microbiology 56 (2006): 495.
  13. Goodfellow M. The Actinomycetes I. Suprageneric classifcation of actinomycetes”. In Bergey's Manual of Systematic Bacteriology 4 (1989): 2333-2339.
  14. Tindall BJ., et al. “Phenotypic characterization and the principles of comparative systematics”. In Methods for General and Molecular Microbiology, 3rd edition (2007): 330-393.
  15. Williams ST., et al. “Genus Streptomyces Waksman and Henrici 1943”. 339 AL. In Bergey’s manual of Sytematic Bacteriology 4 (1989): 2452-2492.
  16. Betina V. “The chemistry and biology of antibiotics”. Amsterdam: Elsevier Scientific Pub. Co; (1983):190.
  17. Andrews JM. “Determination of minimum inhibitory concentrations”. Journal of Antimicrobial Chemotherapy 1 (2001): 5-16.
  18. Hsu SC and Lockwood JL. “Powdered Chitin Agar as a selective medium for enumeration of Actinomycetes in water and soil”. Journal of Applied Microbiology 29 (1975): 422-425.
  19. Smibert RM and Krieg NR. “Phenotypic characterization”. In Methods for general and molecular bacteriology (1994): 607-654.
  20. Stackebrandt E and Ebers J. “Taxonomic parameters revisited: tarnished gold standards”. Microbiology Today 33 (2006): 152-155.
  21. Stackebrandt E and Goebel BM. “Taxonomic Note: A Place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology”. International Journal of Systematic Bacteriology 44 (1994): 846-849.
  22. Kampfer P., et al. “A numerical classification of the genera Streptomyces and Streptoverticellium using miniaturized physiological tests”. Journal of General Microbiology 137 (1991): 1831-1891.
  23. De lima procopio RE. “Antibitics produced by Streptomyces”. The Brzaillian Journal of Infectious Diseases5 (2012): 466-471.
  24. Collee JG and Miles RS. “Tests for identification of bacteria”. In Mackie and McCartney's Practical Medical Microbiology, 13th edition (1989): 141-160.


Citation: Lopamudra Ray., et al. “Extraction and Preliminary Characterization of Potential Antibacterial Agent from Potential Streptomyces cinereoruber sp. Isolated from an Chilika Lake". Acta Scientific Microbiology 3.5 (2020): 13-21.


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