Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 4

Fungi in Biodegradation of Polycyclic Aromatic Hydrocarbons in Oilfield Wastewater

Obire O Aleruchi O* and Wemedo S A.

MD Neurology, India

*Corresponding Author: Department of Microbiology, Rivers State University, Port Harcourt, Nigeria

Received: March 12, 2020; Published: April 01, 2020

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Abstract

 Oilfield wastewater not adequately treated contains organic and inorganic substances including polycyclic aromatic hydrocarbons (PAHs). PAHs are toxic and are not easily degraded by microorganisms. Therefore, this study investigated the role of fungi in the biodegradation of PAHs in oilfield wastewater. Oilfield wastewater was collected from an onshore oil producing platform and biodegradation of polycyclic aromatic hydrocarbons was investigated using standard methods. Fungi were isolated from oilfield wastewater polluted soils obtained from the vicinity of the oil producing platform. The fungal isolates were identified molecularly using internal transcribed spacer (ITS) and identified with accession numbers as Aspergillus niger (MN094359), Aspergillus sydowii (MN094361) and Fusarium lichenicola (MN094360). Experimental control set-up and treatments contained 125 ml of oilfield wastewater. Each of the single treatments had 12.5 ml of mineral salt medium which contained the fungal isolates. Mixed culture treatment contained 4.17 ml each of the fungal isolates while the control set up contained oilfield wastewater only. The experimental samples were periodically analyzed at day 7 and day 21 intervals for polycyclic aromatic hydrocarbon degradation using Gas Chromatography (GC). The total amount of PAHs in oilfield wastewater on day 1 was 101.72992 mg/l. The amount of PAHs in control on day 7 and 21 was 93.01704 mg/l and 75.40663 mg/l, respectively. Aspergillus niger, Aspergillus sydowii, Fusarium lichenicola and mixed culture fungi on day 21 recorded 27.0136, 27.3887, 34.7772 and 15.02925 mg/l with percentage removal of 73.4%, 73.1%, 65.8% and 85.2%, respectively. There was a significant difference between the treatment options and the control on day 7 and 21 at P≤0.05. The results therefore revealed significant reduction of PAHs in the treatment options. In addition, there was complete removal of naphthalene and chrysene by Aspergillus niger and mixed culture while Aspergillus sydowii showed removal of Chrysene only. In conclusion, fungi have great potentials in degradation of PAHs and in remediation of PAHs contaminated environments, different species of fungi have the ability to attack one or more components of PAHs and complete degradation of PAHs was best achieved by mixed fungal cultures.

Keywords: Oilfield Wastewater; PAHs; Fungi; Biodegradation; GC; Naphthalene; Chrysene

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Citation

Citation: Obire O Aleruchi O and Wemedo S A. “Fungi in Biodegradation of Polycyclic Aromatic Hydrocarbons in Oilfield Wastewater". Acta Scientific Microbiology 3.4 (2020): 220-224.



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