Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 4

Evaluation the Use of the lux Reporter System pUC19-luxAB for Rapid and Sensitive Detection of Assimilable Organic Carbon (AOC) in Reverse Osmosis (RO) Feed Water

Thi Tu Uyen Bui*

Faculty of Biology and Biotechnology, University of Science, Hochiminh City, Vietnam

*Corresponding Author: Thi Tu Uyen Bui, Faculty of Biology and Biotechnology, University of Science, Hochiminh City, Vietnam.

Received: February 26, 2020; Published: March 16, 2020

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Abstract

  Assimilable organic carbon (AOC) in reverse osmosis (RO) feed water has been assumed to be the key determinant for microorganism growth leading to membrane fouling and limiting the use of this membrane technology. The lux reporter system developed in this study can be used as a membrane fouling indicator to estimate fouling potential of RO pre-treated feed water. The construction of bioluminescent reporter plasmids was done by integrating luxAB genes from Vibrio fischeri MJ1 downstream of a strong promoter (lacIQ) into high copy number plasmids pUC19. To investigate the use of the lux reporter for detection of AOC, the pUC19-luxAB system was introduced into a common marine bacterium, Vibrio cholerae A1552 by electroporation. The results showed that luxAB genes were successfully expressed in V. cholerae A1552 with the bioluminescence reading of 3.3 x 104 relative light unit (RLU), which was 3000-fold greater than the background signal. The linear curve constructed by A1552 (pUC19-luxAB) with a coefficient (R2) of 0.9761 showed that there was a strong correlation between bioluminescent intensity and glucose concentrations in a range of 0 - 100 µg C L-1. The detection limit of this assay was 20 µg C L-1.

Keywords: Assimilable Organic Carbon (AOC); Lux Reporter System; RO; Fouling Indicator; Bioluminescence Measurement; pUC19-luxAB

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References

  1. Andreu N., et al. “Optimisation of bioluminescent reporters for use with mycobacteria”. PloS One 5.5 (2010): e10777.
  2. Chua C., et al. “Improved sensitivity of the in vivo lux reporter system in measuring reactive oxygen species during antibiotic treatment of Escherichia coli”. Journal of Experimental Microbiology and Immunology 18 (2014): 114-120.
  3. Giwa A and Ogunribido A. “The applications of membrane operations in the textile industry: a review”. Current Journal of Applied Science and Technology 2.3 (2012): 296.
  4. Glascock CB and Weickert MJ. “Using chromosomal lacIQ1 to control expression of genes on high-copy-number plasmids in Escherichia coli”. Gene 223 (1998): 221-231.
  5. Haddix PL., et al. “Characterization of bioluminescent derivatives of assimilable organic carbon test bacteria”. Apply and Environmental Microbiology 70.2 (2004): 850-854.
  6. Iorhemen OT., et al. “Membrane bioreactor (MBR) technology for wastewater treatment and reclamation: membrane fouling”. Membranes 6.2 (2016): 33.
  7. Jablonski E and DeLuca M. “Properties and uses of immobilized light-emitting enzyme systems from Beneckea harveyi”. Clinical Chemistry 25.9 (1979): 1622-1627.
  8. Jeong S., et al. “A rapid bioluminescence-based test of assimilable organic carbon for seawater”. Desalination 317 (2013): 160-165.
  9. LeChevallier MW., et al. “Development of a rapid assimilable organic carbon method for water”. Apply and Environmental Microbiology 59.5 (1993): 1526-1531.
  10. Liu IRIS., et al. “The effect of spheroplast formation on the transformation efficiency in Escherichia coli DH5α”. Journal of Experimental Microbiology and Immunology 9 (2006): 81-85.
  11. Ma J., et al. “Assimilable organic carbon (AOC) in soil water extracts using Vibrio harveyi BB721 and its implication for microbial biomass”. PloS One 7.5 (2012): e28519.
  12. Matz C., et al. “Biofilm formation and phenotypic variation enhance predation-driven persistence of Vibrio cholerae”. Proceedings of the National Academy of Sciences of the United States of America 102.46 (2005): 16819-16824.
  13. Mun E., et al. “Measurements of assimilable organic carbon (AOC) in high saline conditions using P17”. Water Science and Technology Water Supply 13.2 (2013): 265-272.
  14. Seitz P and Blokesch M. “DNA-uptake machinery of naturally competent Vibrio cholerae”. Proceedings of the National Academy of Sciences of the United States of America 110.44 (2013): 17987-17992.
  15. Tehrani GA., et al. “Molecular cloning and expression of the luciferase coding genes of Vibrio fischeri”. African Journal of Biotechnology 10.20 (2011): 4018-4023.
  16. Van Der Kooij D., et al. “Determining the concentration of easily assimilable organic carbon in drinking water”. American Water Works Association 74.10 (1982): 540-545.
  17. Viswanathan K., et al. “Mutational analysis of the Myxococcus xanthus Ω4406 promoter region reveals an upstream negative regulatory element that mediates C-signal dependence”. Journal of Bacteriology 188.2 (2006): 515-524.
  18. Weinrich LA., et al. “Bioluminescence-based method for measuring assimilable organic carbon in pretreatment water for reverse osmosis membrane desalination”. Apply and Environmental Microbiology 77.3 (2011): 1148-1150.
  19. Wiles S., et al. “Alternative luciferase for monitoring bacterial cells under adverse conditions”. Apply and Environmental Microbiology 71.7 (2005): 3427-3432.
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Citation

Citation: Thi Tu Uyen Bui. “Evaluation the Use of the lux Reporter System pUC19-luxAB for Rapid and Sensitive Detection of Assimilable Organic Carbon (AOC) in Reverse Osmosis (RO) Feed Water". Acta Scientific Microbiology 3.4 (2020): 145-151.




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