Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 4

Evaluation the Use of the lux Reporter System pUC19-luxAB for Rapid and Sensitive Detection of Assimilable Organic Carbon (AOC) in Reverse Osmosis (RO) Feed Water

Thi Tu Uyen Bui*

Faculty of Biology and Biotechnology, University of Science, Hochiminh City, Vietnam

*Corresponding Author: Thi Tu Uyen Bui, Faculty of Biology and Biotechnology, University of Science, Hochiminh City, Vietnam.

Received: February 26, 2020; Published: March 16, 2020

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Abstract

  Assimilable organic carbon (AOC) in reverse osmosis (RO) feed water has been assumed to be the key determinant for microorganism growth leading to membrane fouling and limiting the use of this membrane technology. The lux reporter system developed in this study can be used as a membrane fouling indicator to estimate fouling potential of RO pre-treated feed water. The construction of bioluminescent reporter plasmids was done by integrating luxAB genes from Vibrio fischeri MJ1 downstream of a strong promoter (lacIQ) into high copy number plasmids pUC19. To investigate the use of the lux reporter for detection of AOC, the pUC19-luxAB system was introduced into a common marine bacterium, Vibrio cholerae A1552 by electroporation. The results showed that luxAB genes were successfully expressed in V. cholerae A1552 with the bioluminescence reading of 3.3 x 104 relative light unit (RLU), which was 3000-fold greater than the background signal. The linear curve constructed by A1552 (pUC19-luxAB) with a coefficient (R2) of 0.9761 showed that there was a strong correlation between bioluminescent intensity and glucose concentrations in a range of 0 - 100 µg C L-1. The detection limit of this assay was 20 µg C L-1.

Keywords: Assimilable Organic Carbon (AOC); Lux Reporter System; RO; Fouling Indicator; Bioluminescence Measurement; pUC19-luxAB

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Citation

Citation: Thi Tu Uyen Bui. “Evaluation the Use of the lux Reporter System pUC19-luxAB for Rapid and Sensitive Detection of Assimilable Organic Carbon (AOC) in Reverse Osmosis (RO) Feed Water". Acta Scientific Microbiology 3.4 (2020): 145-151.



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