Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 4

Separation of Lignocellulosic Biomass Components by Alkali Pretreatment

Parmeshwar Patil*, Prathamesh Wadekar, Mallikarjun Patil and Arvind Lali

DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology (Formerly UDCT), Mumbai, India

*Corresponding Author: Parmeshwar Patil, DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology (Formerly UDCT), Mumbai, India.

Received: February 13, 2020; Published: March 12, 2020

×

Abstract

  Traditional pretreatment methods have several disadvantages, including lower efficiency in terms of cellulose purity, high cost and incomplete separation of biomass components. Considering this, we compared different pretreatment processes and studied their effect on separation of biomass components. Effective delignification was achieved by alkali pretreatment with sodium hydroxide and ammonia. Among alkalis, sodium hydroxide treatment requires less severe process condition like lower concentration, temperature and pressure compared to ammonia pretreatment and produces enzyme amenable substrate, which made sodium hydroxide ideal for biomass pretreatment. Efforts were mounted to optimize sodium hydroxide pretreatment on wheat straw in terms of reaction temperature, reaction time and reagent concentration to produce enzymatically amenable substrate. The best experimental results were obtained when biomass was treated with 2% sodium hydroxide at 130°C for 30 minutes, which was found to extract lignin and significant amount of hemicellulose from biomass.

Keywords: Biomass; Alkali Pretreatment; Precipitation; Separation

×

References

  1. Z Anwar., et al. “Agro-industrial lignocellulosic biomass a key to unlock the future bio-energy: A brief review”. Journal of Radiation Research and Applied Science 7 (2014): 163-173. 
  2. Y Zheng., et al. “Overview of biomass pretreatment for cellulosic ethanol production”. International Journal of Agricultural and Biological Engineering 2.3 (2009): 51-68. 
  3. M Foston J., et al. “Biomass characterization: recent process in understanding biomass recalcitrance”. Ingram Micro 8 (2012): 4-22. 
  4. JS Kim., et al. “A review on alkaline pretreatment technology for bioconversion of lignocellulosic biomass”. Bioresource Technology (2015).
  5. H Wulfhorst., et al. “Tippkeotter, Compositional analysis of pretreated (beech) wood using differential scanning calorimetry and multivariate data analysis”. Tetrahedron (2016): 1-6. 
  6. SR Decker., et al. “High-throughput screening of recalcitrance variations in lignocellulosic biomass: total lignin, lignin monomers, and enzymatic sugar release”. Journal of Visualized Experiments 103 (2015): e53163.
  7. J Lindedam., et al. “Evaluation of high throughput screening methods in picking up differences between cultivars of lignocellulosic biomass for ethanol production”. Biomass and Bioenergy 66 (2014): 261-267. 
  8. ASluiter B Hames., et al. “Determination of structural carbohydrates and lignin in biomass laboratory analytical procedure”. Golden, CO: National Renewable Energy Laboratory (2008). 
  9. FC Moreira-Vilar., et al. The acetyl bromide method is faster, simpler and presents best recovery of lignin in different herbaceous tissues than klason and thioglycolic acid methods (2014).
  10. Ragauskas AJ., et al. “The path forward for biofuels and biomaterials”. Science 311.5760 (2006): 484-489. 
  11. Yan L., et al. “Hot water pretreatment of lignocellulosic biomass: an efective and environmentally friendly approach to enhance biofuel production”. Chemical Engineering and Technology 39.10 (2016): 1759-1770. 
  12. Pu Y., et al. “Assessing the molecular structure basis for biomass recalcitrance during dilute acid and hydrothermal pretreatments”. Biotechnol Biofuels 6.1 (2013): 1.
  13. Meng X., et al. “An in-depth understanding of biomass recalcitrance using natural poplar variants as the feedstock”. Chem Sus Chem 10.1 (2016): 139-150.
  14. Chang VS and Holtzapple MT. “Fundamental factors affecting biomass enzymatic reactivity”. Applied Biochemistry and Biotechnology 84 (2000): 5-37.
  15. A Sluiter., et al. “Determination of structural carbohydrates and lignin in biomass laboratory analytical procedure”. Golden, CO: National Renewable Energy Laboratory (2008).
×

Citation

Citation: Parmeshwar Patil., et al. “Separation of Lignocellulosic Biomass Components by Alkali Pretreatment". Acta Scientific Microbiology 3.3 (2020): 123-128.




Metrics

Acceptance rate30%
Acceptance to publication20-30 days

Indexed In






News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is December 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"

Contact US