Justine Maïwore1*, Daouda Youssouf1, Alhadji Toudjani Amadou1, Martin-Paul Baane2, Léopold Tatsadjieu Ngoune3 and Didier Montet4
1Department of Life and Earth Sciences, University of Maroua, Higher Teachers’ Training College, Maroua, Cameroon
2Laboratory of the hospital of National Social Insurance Fund (NSIF), Maroua, Cameroon
3Department of Food Engineering and Quality Control, University Institute of technology, University of Ngaoundere, Ngaoundere, Adamaoua, Cameroon
4UMR 95 Quali Sud/Cirad/University of Montpellier, TAB-95/16, 73 rue J-F Breton, Montpellier Cedex, France
*Corresponding Author: Justine Maïwore, Department of Life and Earth Sciences, University of Maroua, Higher Teachers’ Training College, Maroua, Cameroon.
Received: February 24, 2020; Published: March 06, 2020
Raw cow’s milk produced locally is a popular commodity but, its quality is a hindrance for the consumers. The objective of this study was firstly to identify the Staphylococcus present in raw cow’s milk collected in Maroua (Cameroon) and, secondly to assess their level of resistance to antibiotics. Physico-chemical analyses based on the temperature, pH and titrable acidity were carried out. For the microbial analysis, the plating method was used to isolate and, the count of the total mesophilic aerobic flora (TMAF) and Staphylococcus was done. After identification, the resistance of Staphylococcus to antibiotics was assessed. The results obtained revealed that the temperature value was between 34.3 ±0.282 and 36 ±0.212°C; the pH between 5.18± 0.32 and 5.80± 0.2 and the density between 1.025±0.0007 and 1.030± 0.0021. Microbiological analyses revealed that TMAF ranged from (9± 0.9)103 to (7.2± 0.9)109 CFU/mL while the abundance of Staphylococcus ranged from (1.2 ± 0.3)103 to (1.03± 0.08)104 CFU/mL. Nineteen (19) species of Staphylococcus were identified with a predominance of the aureus specie (68.62%). The antibiogram revealed 95% resistance to β-lactam, 78% to Macrolides, 42% to Glycopeptides, 16% to Quinolones, 5% to Aminosides and Cotrimoxazole and 0% to Chloramphenicol. Raw milk from Maroua has a high rate of Staphylococcus antibiotic-resistant.
Keywords: Raw Milk; Staphylococcus; Antibiotics; Resistance; Maroua
Citation: Justine Maïwore., et al. “Staphylococcus in Raw Cow's Milk from Maroua (Cameroon), Determination of Their Resistance Level to Antibiotics". Acta Scientific Microbiology 3.4 (2020): 44-52.
Copyright: © 2020 Justine Maïwore., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.