Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 3

Determination of the Biochemical Profile of Bacillus thuringiensis Strains Isolated from Middle Tennessee

Fahdah Alshammari1,2*, Terrance Johnson1 and Anthony Ejiofor1

1Department of Biological Sciences, Tennessee State University, USA
2Department of Microbiology, Northern Border University, Saudi Arabia

*Corresponding Author: Fahdah Alshammari, Department of Biological Sciences, Tennessee State University, USA.

Received: January 22, 2020; Published: February 14, 2020

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Abstract

  There is an ever growing need for control of insects of agricultural and health importance. The practice of using chemicals for this purpose has been flawed due to many untoward fall outs including insect resistance, non-target toxicity and non-biodegradability. A promising and viable practice is the use of biological agents such as Bacillus thuringiensis (Bt) that are efficacious as well as safe. Bacillus thuringiensis (Bt) was first isolated in the early 1900s, and interest in it and its used have soared as a result of its insecticidal capability. Bt is a Gram-positive, endospore-forming organism that produces crystal proteins that serve as toxins to select insects. Thus, its use as a bioinsecticide has exploded prompting the search for newer isolates and the establishment of culture collections of Bt strains in many laboratories. The purpose of the work presented here was to assess the biochemical profile of 72 strains collected in Middle Tennessee as well as those of two standard strains, B. t. israelensis (BtI) and B. t. kurstaki (BtK) as a method for their characterization. The approach was to determine the biochemical profile of each strain using the Biolog Identification System, which allowed for the assessment of the metabolic activity of each strain in the presence of 95 different substrates. It was found that the strains clustered into two major groups having common substrates, and these groups gave two and three sub-clusters. Thus, it was concluded that the strains of this study had varying metabolic ability for the substrates provided in the Biolog Gen III Microplates; however, there were certain substrates that were commonly used by the strains used in this study.

Keywords: Bacillus thuringiensis; Biolog Identification System; Gen III Microplates; Biochemical Profile

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Citation

Citation: Fahdah Alshammari., et al. “Determination of the Biochemical Profile of Bacillus thuringiensis Strains Isolated from Middle Tennessee". Acta Scientific Microbiology 3.3 (2020): 01-05.



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