Suleiman Kafilat Olayemi* and Usman Aliyu Dutsinma
Department of Microbiology, Faculty of Life Science, Bayero University, Kano, Nigeria
*Corresponding Author: Suleiman Kafilat Olayemi, Department of Microbiology, Faculty of Life Science, Bayero University, Kano, Nigeria.
Received: January 06, 2020; Published: February 08, 2020
Rotavirus is the most common cause of diarrhoeal disease among infants and young children. It is a genus of double-stranded RNA viruses in the family Reoviridae. According to literatures, nearly every child in the world is infected with rotavirus at least once by the age of five. The study was carried out to determine the incidence of Rotavirus among children (0 - 5years) suffering from diarrhoea attending Hasiya Bayero Pediatric Hospital, Kano state. Two hundred and ninety-nine children within ages 0 - 5 years who were at Hasiya Bayero Pediatric Hospital, Kano, at the time of the study were enrolled in the study. Diarrhoea samples were obtained following parental consent and ethical approval from the medical research ethics committee of the hospital. The stool samples were collected aseptically in sterile commercial stool containers adequately labeled (patient ID and date of collection) and were transported in ice to the Center for Biotechnology Research, Bayero University Kano where they we restored at −200C until tested. The presence of Rotavirus was detected using RDT and ELISA (IgM) in the stool samples. Rotavirus (IgM) was detected in 22 of the 299 subjects giving an incidence of 7.4% using ELISA. The RDT kit detected Rotavirus in 45 of the 299 subjects giving an incidence of 15.1%. Female subjects had higher incidence (51.1%) compared to male subjects (48.9%). Subjects in age group 13-24 months old had the highest incidence of Rotavirus (5.0% and 3.7%) while subjects in age group 25 - 36 months old had the lowest percent positive (0.7% and 0.3%) for RDT and ELISA respectively. The socio-demographic variables associated with the incidence of Rotavirus in this study were mother’s level of education, breastfeeding practices and method of water storage. ELISA test kit was found to be more specific and sensitive when compared with Rapid test kit used in this study in detecting Rotavirus. It is recommended that mothers should be educated through their anti-natal and post-natal clinics on the process of avoiding the infection through effective hygiene especially when babies are between 0-5years old especially 13 - 24 months and the detection of Rotavirus among diarrhoea patients should focus more on the use of ELISA instead of RDT.
Keywords: Rotavirus; Diarrhoea; Children
Citation: Suleiman Kafilat Olayemi and Usman Aliyu Dutsinma. “Incidence of Rotavirus Among Children with Diarrhoea Attending Hasiya Bayero Pediatric Hospital, Kano State, Nigeria". Acta Scientific Microbiology 3.3 (2020): 01-08.
Copyright: © 2020 Suleiman Kafilat Olayemi and Usman Aliyu Dutsinma. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.