Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 2

Isolation and Characterization of Extremophilies from Shalla/Abidjata Hot Springs, Ethiopia

Eyob Kassaye Wolella1* and Belay Tilahun2

1Department of Biology, Natural and Computational Science, Debre Tabor University, Debre Tabor, Ethiopia
2Department of Biotechnology, Natural and Computational Science, Wolkite University, Wolkite, Ethiopia

*Corresponding Author: Eyob Kassaye Wolella, Department of Biology, Natural and Computational Science, Debre Tabor University, Debre Tabor, Ethiopia.

Received: December 06, 2019; Published: January 20, 2020

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Abstract

  The purpose of this study was to isolate and characterize Extremophilic species from geothermal areas of Ethiopian rift valley where the water temperature was 95 - 100 °C and the pH was around 6.5 - 8. About 1.5 ml water and 1 gram sediments were cultured in both Castenholz TYE media and nutrient media. To evaluate the thermo-tolerant characteristics and effects of pH the isolates grew at 60-95 ºC with optimum temperature of at 70 ºC and pH range of 6.0 - 9.5 with optimum pH of 7.5. Three isolates were screened based on the growth characterization at elevated temperature. Isolates NMS and NL2I were aerobic, gram negative, non motile and whitish and TMI were gram positive, motile and whitish. Based on the phenotypic characterization the thermophilic isolate designated as NMS, NL2I and TMI were found to be closely-related to the genus Thermus spp and Bacillus spp respectively. This study is useful in providing characteristic of indigenous thermopiles that are potential for growth of biotechnology industry.

Keywords: Hot Springs; Extremophiles; Bacillus Spp And Thermus Spp

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Citation

Citation: Eyob Kassaye Wolella and Belay Tilahun. “Isolation and Characterization of Extremophilies from Shalla/Abidjata Hot Springs, Ethiopia". Acta Scientific Microbiology 3.2 (2020): 65-69.



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