Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Short Report Volume 3 Issue 2

Failure to Detect Hantavirus in Vesper Bats in Poland

Satoru Arai1, Fuka Kikuchi1, Janusz Hejduk2, Janusz Markowski2, Keiko Tanaka-Taya1, Beata Sikorska3, Shigeru Morikawa4, Motoi Suzuki1, Paweł P Liberski4, and Richard Yanagihara5*

1Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo, Japan
2Department of Teacher Training and Biodiversity Studies, Faculty of Biology and Environmental Protection, University Of Łódź, Poland
3Department of Molecular Pathology and Neuropathology, Medical University of Łódź, Łódź, Poland
4Faculty of Veterinary Medicine, Okayama University of Science, Ehime, Japan
5Departments of Pediatrics and Tropical Medicine, Medical Microbiology and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, USA

*Corresponding Author: Richard Yanagihara, Departments of Pediatrics and Tropical Medicine, Medical Microbiology and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, USA.

Received: December 23, 2019; Published: January 06, 2019

×

Abstract

Objective: We previously reported the co-circulation of genetically distinct hantaviruses (family Hantaviridae) in shrews and moles (order Eulipotyphla, families Soricidae and Talpidae) in central and southeastern Poland. In this exploratory study, we attempted to detect hantavirus in patagia of vesper bats (order Chiroptera, family Vespertilionidae) from Poland.

Methods: Ethanol-fixed patagia from 88 vesper bats, collected during 2013, 2014 and 2017 in northern, central and south-central Poland, were analyzed for hantavirus RNA by nested reverse transcription polymerase chain reaction (RT-PCR).

Results and Discussion: Despite repeated and exhaustive attempts, using oligonucleotide primers and PCR cycling conditions that led to the discovery of bat-borne hantaviruses in Asia and Africa, we failed to detect hantavirus RNA in vesper bats from Poland, including the common noctule (Nyctalus noctula), which has recently been shown to harbora hantavirus in the Czech Republic. More extensive studies of visceral organs from bats in Poland and elsewhere in Europe are warranted to ascertain the genetic diversity and phylogeography of bat-borne hantaviruses.

Keywords: Hantaviridae; Vesper Bat; Chiroptera; Poland; RT-PCR

×

References

  1. YanagiharaR., et al. “Hantaviruses: rediscovery and new beginnings”. Virus Research 187 (2014): 6-14.
  2. Bennett SN., et al. “Reconstructing the evolutionary origins and phylogeography of hantaviruses”. Trends in Microbiology 22 (2014): 473-482.  
  3. Song JW., et al. “Seewis virus, a genetically distinct hantavirus in the Eurasian common shrew (Sorex araneus)”. Virology Journal 4 (2007): 114.
  4. Kang HJ., et al. “Genetic diversity and phylogeography of Seewis virus in the Eurasian common shrew in Finland and Hungary”. Virology Journal 6 (2009): 208. 
  5. Gu SH., et al. “Boginia virus, a newfound hantavirus harbored by the Eurasian water shrew (Neomys fodiens) in Poland”. Virology Journal 10 (2013): 160. 
  6. Gu SH., et al. “Co-circulation of soricid- and talpid-borne hantaviruses in Poland”. Infection Genetics and Evolution 28 (2014): 296-303. 
  7. Schlegel M., et al. “Broad geographical distribution and high genetic diversity of shrew-borne Seewis hantavirus in Central Europe”. Virus Genes 45 (2012): 48-55. 
  8. Klempa B., et al. “The broad spectrum of hantaviruses and their hosts in Central Europe”. Acta Virologica 57 (2013): 130-137.
  9. Resman K., et al. “Molecular evidence and high genetic diversity of shrew-borne Seewis virus in Slovenia”. Virus Research 177 (2013): 113-117. 
  10. Radosa L., et al. “Detection of shrew-borne hantavirus in Eurasian pygmy shrew (Sorex minutus) in Central Europe”. Infection Genetics and Evolution 19 (2013): 403-410. 
  11. Arai S and Yanagihara R. “Genetic diversity and geographic distribution of bat-borne hantaviruses”. In: Corrales-Aguilar E and Schwemmle M., eds. Bat-borne Viruses. Caister Academic Press, Poole, United Kingdom 59-86 (2019).
  12. Arai S., et al. “Novel bat-borne hantavirus, Vietnam”. Emerging Infectious Diseases 19 (2013): 1159- 1161. 
  13. Gu SH., et al. “Molecular phylogeny of hantaviruses harbored by insectivorous bats in Côte d’Ivoire and Vietnam”. Viruses 6 (2014): 1897-1910. 
  14. Arai S., et al. “Molecular phylogeny of mobatviruses (Hantaviridae) in Myanmar and Vietnam”. Viruses 11 (2019): 228. 
  15. Arai S., et al. “Đakrông virus, a novel mobatvirus harbored by the Stoliczka’s Asian trident bat (Aselliscus stoliczkanus) in Vietnam”. Scientific Reports 9 (2019): 10239. 
  16. Xu L., et al. “Novel hantavirus identified in black-bearded tomb bats, China”. Infection Genetics and Evolution 31 (2015): 158-160. 
  17. Guo WP., et al. “Phylogeny and origins of hantaviruses harbored by bats, insectivores, and rodents”. PLoS Pathogens 9 (2013): e1003159. 
  18. Zana B., et al. “Molecular Identification of a novel hantavirus in Malaysian bronze tube-nosed bats (Murina aenea)”. Viruses 11 (2019): 887. 
  19. Arai S., et al. “Molecular phylogeny of a genetically divergent hantavirus harbored by the Geoffroy’s rousette (Rousettus amplexicaudatus), a frugivorous bat species in the Philippines”. Infection Genetics and Evolution 45 (2016): 26-32. 
  20. Sumibcay L., et al. “Divergent lineage of a novel hantavirus in the banana pipistrelle (Neoromicia nanus) in Côte d’Ivoire”. Virology Journal 9 (2012): 34. 
  21. Těšíková J., et al. “Hantavirus strains in East Africa related to Western African hantaviruses”. Vector Borne and Zoonotic Diseases 17 (2017): 278-280. 
  22. Weiss S., et al. “Hantavirus in bat, Sierra Leone”. Emerging Infectious Diseases 18 (2012): 159-161.
  23. Witkowski PT., et al. “Phylogenetic analysis of a newfound bat-borne hantavirus supports a laurasiatherian host association for ancestral mammalian hantaviruses”. Infection Genetics and Evolution 41 (2016): 113-119.                
  24. Straková P., et al. “Novel hantavirus identified in European bat species Nyctalus noctula”. Infection Genetics and Evolution 48 (2017): 127-130. 
  25. Worthington Wilmer J and Barratt E. “A non-lethal method of tissue sampling for genetic studies of chiropterans”. Bat Research News 37 (1996): 1-3.
  26. Flanders J., et al. “Phylogeography of the greater horseshoe bat, Rhinolophus ferrumequinum: contrasting results from mitochondrial and microsatellite data”. Molecular Ecology 18 (2009): 306-318. 
  27. Vázquez-Domínguez E., et al. “High dispersal and generalist habits of the bat Artibeus jamaicensis on Cozumel Island, Mexico: an assessment using molecular genetics”. Acta Chiropterologica 15 (2013): 411-421.
  28. Corthals A., et al. “From the field to the lab: best practices for field preservation of bat specimens for molecular analyses”. Plos one 10 (2015): e0118994. 
  29. Ignaczak M and Postawa T. “Protection of the Szachownica cave as an example of saving a valuable bat wintering shelter”. Proceedings of the Theriological School 15 (2017): 67-74.
  30. Kang HJ., et al. “Evolutionary insights from a genetically divergent hantavirus harbored by the European common mole     (Talpa europaea)”.  Plos   one 4 (2009): e6149. 
  31. Gu SH., et al. “Complete genome sequence and molecular phylogeny of a newfound hantavirus harbored by the Doucet’s musk shrew (Crocidura douceti) in Guinea”. Infection Genetics and Evolution 20 (2013): 118-123. 
  32. Klempa B., et al. “Hantavirus in African wood mouse, Guinea”. Emerging Infectious Diseases 12 (2006): 838-840. 
×

Citation

Citation: Satoru Arai., et al. “Failure to detect Hantavirus in Vesper Bats in Poland". Acta Scientific Microbiology 3.2 (2020): 07-11.



Indexed In






News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is December 15, 2020.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of “Best Article of the Issue”.
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.
  • Contact US