Abstract

   Experiments on random-bred albino mice showed that the acute intoxication of anticholinesterase compounds (DDVP, 0.75 LD₅₀) is implemented the cholinergic anti-inflammatory pathway (reduced mortality of mice from sepsis and the blood concentration of proinflammatory cytokines TNF-α, IL-1β, IL-6). Sepsis caused by the intraperitoneal administration of saline diurnal culture of E. coli O157:H7 (2.5×109 CFUs). The use of the cholinesterase reactivator сarboxim (15 mg/kg) - antidote of organophosphorus compounds - after acute intoxication of DDVP diminished affect the implementation of the cholinergic anti-inflammatory pathway.

Keywords: Anti-Inflammatory Pathway; Sepsis; E. Coli; Anticholinesterase Compounds; Cholinesterase Reactivator Сarboxim

References

1. Tremolada P., et al. “Quantitative inter-specific chemical activity in the aquatic environment”.  Aquatic Toxicology1 (2004): 87-103.
2. Zabrodskii PF. “Effect of armin on nonspecific resistance factors of the body and on the primary humoral immune response”. Farmakologiia I Toksikologiia1 (1987): 57-60.
3. Zabrodskii PF. “Variation in antiinfectious nonspecific resistance of the organism caused by cholinergic stimulation”. Bulletin of Experimental Biology and Medicine2 (1995): 809-811.
4. Zabrodskii PF. “Change in the non-specific anti-infection resistance of the body exposed to cholinergic stimulation”. Bulletin of Experimental Biology and Medicine8 (1995): 164-166.
5. PF Zabrodskii. “Immunotoxicology of organophosphorus compounds”. Saratov (2016): 289.
6. Zabrodskii PF and Germanchuk VG. “Role of activation of the sympathoadrenal system during the poisoning of organophosphorus compounds”. Bulletin of Experimental Biology and Medicine4 (2001): 966-968.
7. Zabrodskii PF., et al. “Role of anticholinesterase mechanism in suppression of antibody formation during acute poisoning with organophosphorus compounds”. Bulletin of Experimental Biology and Medicine5 (2001): 467-469.
8. Zabrodskii PF and Mandych VG. “Immunotoxicology of xenobiotics”. Saratov Military Institute of Biological and Chemical Safety (2007): 420.
9. Bernik TR., et al. “Pharmacological stimulation of the cholinergic antiinflammatory pathway”. Journal of Experimental Medicine 6 (2002):781-788.
10. Borovikova LV., et al. “Vagus nerve stimulation attenuates the systemic inflammatory response to endotoxin”.  Nature6785 (2000): 458-462.
11. Bonaz BL and Bernstein CN. “Brain-gut interactions in inflammatory bowel disease”. Gastroenterology.144.1 (2013): 36-49.
12. Fernandez R., et al. “Neural reflex regulation of systemic inflammation: potential new targets for sepsis therapy”.  Frontiers in Physiology 5 (2014): 489.
13. Reardon C. “Neuro-immune interactions in the cholinergic anti-inflammatory reflex”. Immunology Letters 178 (2016): 92-96.
14. Wang H., et al. “Nicotinic acetylcholine receptor alpha7 subunit is an essential regulator of inflammation”. Nature6921 (2003): 384-388.
15. Zabrodskii PF. “Effect of acetylcholine on mortality of mice from sepsis and proinflammatory cytokine production”. Bulletin of Experimental Biology and Medicine3 (2011): 340-342.
16. Zabrodskii PF., et al. “Effect of α7n-Acetylcholine Receptor Activation and Antibodies to TNF-α on Mortality of Mice and Concentration of Proinflammatory Cytokines During Early Stage of Sepsis”. Bulletin of Experimental Biology and Medicine6 (2015): 740-742.
17. Zabrodskii PF., et al. “Role of β2-adrenoreceptors in adrenergic anti-Inflammatory mechanism in sepsis”. Bulletin of Experimental Biology and Medicine12 (2016): 718-721.
18. Zabrodskii PF., et al. “Combined Effects of M1 Muscarinic Acetylcholine Receptor Agonist TBPB and α7n-Acetylcholine Receptor Activator GTS-21 on Mouse Mortality and Blood Concentration of Proinflammatory Cytokines in Sepsis”. Bulletin of Experimental Biology and Medicine6 (2017): 750-753.
19. Zabrodskii PF., et al. “Combined Effect of NF-κB Inhibitor and β2-Adrenoreceptor Agonist on Mouse Mortality and Blood Concentration of Proinflammatory Cytokines in Sepsis”. Bulletin of Experimental Biology and Medicine6 (2018): 445-448.
20. Song DJ., et al. “Effect of lentiviral vector encoding on triggering receptor expressed on myeloid cells 1 on expression of inflammatory cytokine in septic mice infected by Bacteroides fragilis”. Zhonghua Shao Shang Za Zhi 1 (2009): 36-41.
21. Eftekhari G., et al. “Activation of central muscarinic receptor type 1 prevents development of endotoxin tolerance in rat liver”. European Journal of Pharmacology 740 (2014): 436-441.

Citation

Citation: Pavel Franzevich Zabrodskii. "The Cholinergic Anti-Inflammatory Pathway. Combined Effect of Anticholinesterase Compounds and its Antidote Cholinesterase Reactivator Carboxim".Acta Scientific Microbiology 3.1 (2020): 70-73.

Copyright

Copyright: © 2020 Pavel Franzevich Zabrodskii. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.