Abstract

   The main purpose of our work was the superior use of the paper and cellulose industry’s wastes by using them as substrate in an optimized culture media formula for the yeasts strains development.

  The best results were obtained on culture media with 35% wood hydrolyzed and also with well determined concentrations of sugar beet molasses and mineral supplements.

  The yeasts growth were verified by optical density (determination meaning the cells number which are present in a well determined liquid volume), the reducing sugar’s consumption and by the Ph dynamics. The results obtained with these yeasts strains selected from different natural sources and named D4 and D5, were compared with those obtained by using identified yeasts strains kept in the microbial collection of our institute, such as Candida robusta and Candida tropicalis.

  Our conclusion indicates that different types of hydrolyzed wood wastes can be recycled by microbiological ways, so that by so called “Green technologies” which seems to act friendly to our environment, while one of our main tasks was to use materials and technologies as natural as possible.

Keywords: Yeasts; Hydrolyzed Wood; Media Composition

References

1. Lee VG., et al. “Ethanol production using concentrated oak wood hydrolysates and methods to detoxify”. Applied Biochemistry and Biotechnology 77-79 (1999): 547-559.
2. Parawira W and Tekere M. “Biotechnological strategies to overcome inhibitors in lignocellulose hydrolysates for ethanol production: review”. Critical Reviews in Biotechnology 1 (2011): 20-31.
3. Kadar Z., et al. “Ethanol fermentation of various pretreated and hydrolyzed substrates at low initial pH”. Applied Biochemistry and Biotechnology 137-140 .1-12 (2007): 847-858.
4. Yu J., et al. “Ethanol production from H (2) SO (3)-steam-pretreated fresh sweet sorghum stem by simultaneous saccharification and fermentation”. Applied Biochemistry and Biotechnology 2 (2010):401-409.
5. Dowe N. “Assessing cellulase performance on pretreated lignocellulosic biomass using saccharification and fermentation-based protocols”. Methods in Molecular Biology 581 (2009): 233-245.
6. Kumar R Shweta. “Enhancement of wood waste decomposition by microbial inoculation prior to vermicomposting”. Bioresource Technology 2 (2011): 1475-1480.
7. Vempalli Sudharsan and Varma Suminakshi Das. “Microbial degradation of lignocellulosic fractions during drum composting of mixed organic waste”. Sustainable Environment Research6 (2017): 265-272.
8. Justyna Bohacz. “Microbial strategies and biochemical activity during lignocellulosic waste composting in relation to the occurring biothermal phases”. Journal of Environmental Management 206 (2018): 1052-1062.
9. Jiajin Liang., et al. “A new screened microbial consortium OEM2 for lignocellulosic biomass deconstruction and chlorophenols detoxification”. Journal of Hazardous Materials 347 (2018): 341-348.
10. Suman Yadav and Suresh Kumar Dubey. “Cellulose degradation potential of Paenibacillus lautus strain BHU3 and its whole genome sequence”. Bioresource Technology 262 (2018): 124-131.
11. Anna Pennachio., et al. “Isolation of new cellulase and xylanase producing strains and application to lignocellulosic biomasses hydrolysis and succinic acid production”. Bioresource Technology 259 (2018): 325-333.
12. Bhanu Pratap Prajapati., et al. “Characterization of cellulase from Aspergillus tubingensis NKBP-55 for generation of fermentable sugars rom agricultural residues”. Bioresource Technology 250 (2018): 733-740.
13. Faizan Qadir., et al. “Evaluation of a yeast co-culture for cellulase and xylanase production under solid state fermentation of ugarcane bagasse using multivariate approach”. Industrial Crops and Products 123 (2018): 407-415.
14. Nayara Fernanda Lisboa Garcia., et al. “Catalytic properties of cellulases and hemicellulases produced by Lichtheimia ramosa: Potential for sugarcane bagasse saccharification”. Industrial Crops and Products 122 (2018) 49-56.
15. Wen Wang., et al. “Effect of stepwise lignin removal on the enzymatic hydrolysis and cellulase adsorption”. Industrial Crops and Products 122 (2018): 16- 22.

Citation

Citation: Ana Despina Ionescu., et al. “Screening of Some Culture Media Compositions for Obtaining the Optimal Yeast Strains Development on Different Natural Sources of Wood Wastes (Wood Hydrolyzed)".Acta Scientific Microbiology 3.1 (2020): 66-69.

Copyright

Copyright: © 2020 Lia Ana Despina Ionescu., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.