Abstract

  By definition, Endophytic fungi are the fungi that spend a part or whole of their life cycle. These fungi colonize the healthy tissues of host plants either inter or intracellularly typically without causing apparent symptoms of disease and without any immediate, negative overt results with the only exception that the fruit bearing structure of these endophytes might emerge out of the plant tissue for reproduction. A newly emerged leaf or any part of the plant may be colonized by a variety of fungal colonies in an asymptomatic or in some cases, symbiotic manner. The main objective of the current study is to scientifically validate the antioxidant activity of methanolic and aqueous extracts of endophytic fungi. Antioxidants are those that inhibit the oxidation by controlling the production of free radicals, thereby stopping the chain reaction that may damage the cells of the organism. The plant endophytes under investigation were Phoma sp., Colletotrichum spiralis, Chaetomium sp. (1) and Chaetomium sp. (2). Endophytes were isolated from plants and were cultured in Potato dextrose broth. The dry biomass yield of all the four endophytes was subjected for the study. The aqueous and methanolic extracts of the four endophytes were subjected to further investigation The total phenolic content of the four different fungal extracts were determined by using Folin-ciocalteau reagent method. DPPH (1,1- diphenyl-2-picryl-hydrazyl) radical assay was performed to determine the percentage of radical scavenging activity for all the four analytes. A general increase in the antioxidant activity was observed in methanolic extracts when compared with aqueous extracts.

Keywords: Endophytes; Antioxidants; Free Radicals; Folin-Ciocalteau; DPPH Radical Assay

References

1. Azevedo JL., et al. “Endophytic microorganism- A review on insect control and recent advances on tropical plants”. Electronic Journal of Biotechnology 3 (2001): 1-36.
2. Zang W., et al. “Mini review of recent W.O patents (2004-2005) of novel antifungal compounds in the field of anti-infective drug targets”. Recent Patents on Anti Infective Drug Discovery 1 (2006): 225-230.
3. Bandara WMMS., et al. “Interactions among endophytic bacteria and fungi: effects and potentials”. Journal of Bioscience 5 (2006): 645-650.
4. Verma V.C., et al. “The endophytic microflora of bark, leaf and stem tissues of Azadirachta indica A. Juss and impact of culture media on their isolation”. Microbial Ecology 54 (2007): 119-125.
5. Kharwar RN., et al. “The endophytic fungal complex of Catharanthus roseus”. Current Science 95 (2008): 228-232.
6. Lϋa JM., et al. “Chemical and molecular mechanism of antioxidants: experimental approaches and model systems”. Journal of Cellular and Molecular Medicine 14 (2010): 840-860.
7. Sen CK. “Oxygen toxicity and antioxidants: state of the art”. Indian Journal of Physiology and Pharmacology 39 (1995): 177‐196.
8. Hegde K and Joshi AB. “Hepatoprotective effect of Carissa carandas Linn root extract against CCl4 and paracetamol induced hepatic oxidative stress”. Indian journal of experimental biology 47 (2009): 660‐667.
9. Sun C., et al. “Free radical scavenging and antioxidant activities of EPS2, an exopolysaccharide produced by a marine filamentous fungus Keissleriella sp. YS 4108”. Life Sciences 75 (2004): 1063-1073.
10. Song FL., et al. “Total phenolic contents and antioxidant capacities of select Chinese medicinal plants”. International Journal of Molecular Science 6 (2010): 2362-2372.
11. Radulovic N., et al. “Screening of in vitro antimicrobial and antioxidant activity of Nine Hypericum species from the Balkans”. Food Chemistry 103 (2007): 15-21.
12. Ames BN., et al. “The causes and prevention of cancer”. Proceedings of the National Academy of Sciences 92 (1995): 5258-5265.
13. Sultan S., et al. “Bioactive fungal metabolites of 9PR2 isolated from roots of Callophyllum ferrrugineum”. International Journal of Pharmacy and Pharmaceutical Sciences 3 (2011): 7-9.
14. Wilson D. “Endophyte- the evolution of a term and clarification of its use and definition”. Okios 73 (1995): 274-276.
15. Tan RX and Zou WX. “Endophytes: a rich source of functional metabolites”. Natural Product Reports 18 (2010): 448-459.
16. Amitha V., et al. “Fungal endophytes from the culinary herbs and their antioxidant activity”. International Journal of Current Research8 (2014): 7996-8002.
17. Volluri S S., et al. “In vitro antioxidant activity and estimation of total phenolic content in methanolic extract of Bacopa moniera”. RASAYAN Journal of Chemistry 4 (2011): 381-386.
18. Pawle G and Singh SK. “Antimicrobial, antioxidant activity and phytochemical analysis of an endophytic species of Nigrospora isolated from living fossil Ginkgo biloba”. Current Research in Environmental and Applied Mycology1 (2014): 1-9.
19. Sultana B., et al. “Antioxidant activity of phenolic compounds present in barks of Azadirachta Indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam trees”. Food Chemistry 104 (2007): 1106-1114.
20. Wong C., et al. “A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay”. Food Chemistry 97 (2006): 705-711.
21. Zou WX., et al. “Metabolites of Colletotrichum gloeosporioides, an Endophytic Fungus in Artemisia mongolica”. Journal of Natural Products 63 (2000): 11.
22. Photita W., et al. “Endophytic fungi of wild banana (Musa acuminata) At Doi Suthep Pui National Park, Thailand”. Mycological Research 105 (2001): 1508-1513.
23. Lu H., et al. “New bioactive metabolites produced by Colletotrichum sp. an endophytic fungus in Artemisia annua”. Plant Science 151 (2000): 76-73.
24. Adriana Maria Fernandes de Oliveira, Lilian Sousa Pinheiro, Charlane Kelly Souto Pereira, Wemerson Neves Matias, Roosevelt Albuquerque Gomes, Otemberg Souza Chaves, Maria de Fátima Vanderlei de Souza, Reinaldo Nóbrega de Almeida and Temilce Simões de Assis Antioxidants 1 (2012): 33-43.
25. Bischoff JF., et al. “Evolutionary development of the Clavicipitacea”. The Fungal Community, Its Organisation and Role in the Ecosystem 2 (2005): 505-518.
26. Brundrett MC., et al. “Microbial root endophytes”. Springer-Verlag (2006): 281-293.
27. Cyd E Hamilton., et al. “Endophytic mediation of reactive oxygen species and antioxidant activity in plants: a review”. Fungal Diversity 54 (2012): 1-10.
28. Daija Krhn K., et al. “A review: natural products associated with plant endophytic fungi”. European Journal of Organic Chemistry 21 (2005): 5100-5105.
29. Davis EC., et al. “Endophytic Xylaria among liverworts and angiosperms: phylogenetics, symbiosis and distribution”. American Journal of Botany (2003): 1661-1667.
30. Davis EC and AJ Shaw. “Biogeographic and phylogenetic patterns in diversity of liverwort associated endophytes” American Journal of Botany 95 (2008): 914-924.
31. Escarpa A and Gonza´lez MC. “Approach to the content of total extractable phenolic compounds from different food samples by comparison of chromatographic and spectrophotometric methods”. Analytica Chimica Acta 427 (2001): 119-127.
32. Faeth SH., et al. “Temporal and spatial variation in alkaloid levels in Achnatherum robustum, a native grass infected by the endophyte Neotyphodium”. Journal of Chemical Ecology 32 (2006): 307-324.
33. Faeth SH. “Are endophytic fungi generally plant mutualists?”. Oikos 98 (2002): 25-36.
34. Fatimah ZI., et al. “Effect on estrogen and palm vitamin F on malonaldehyde levels toward the development of arteriosclerosis in the New Zealand white rabbit”. In: Packer L, Ong SH, editors. Biological Oxidants and antioxidants: Molecular Mechanism and Health Effects., Champaign IL: AOCS Press (1998): 22.
35. Gangadevi V and J Muthumary. “Taxol production by Pestalotiopsis”. Biotechnology and Applied Biochemistry 52 (2009): 9-15.
36. Giri R Genwali., et al. “Isolation of Gallic acid and estimation of total phenolic content in some medicinal plants and their antioxidant activity”. Nepal Journal of Science and Technology1 (2013): 95-102.
37. Higgins KL., et al. “Phylogenetic relationships, host affinity and geographic structure of boreal and arctic endophytes from three major plant lineages”. Molecular Phylogenetics and Evolution 42 (2007): 543-555.
38. Hirsch GU and Braun U. Communities of parasitic microfungi, Handbook of vegetation science: fungi in vegetation science, Kluwer academic, Dorrecht, Netherlands, 19 (1992): 225-250
39. Jumpponen A. “Dark-septate endophytes, are they mycorrhizal?” Mycorrhiza 11 (2001): 207-211.
40. Kharwar RN., et al. “Javanicin, an antibacterial naphthaquinone from an endophytic fungus of neem, Chloridium sp”. Current Microbiology 58 (2009): 233-238.
41. Krings M., et al. “Fungal endophytes in a 400-million-year old land plant: Infection pathways, spatial distribution and host responses”. New Phytologist 174 (2007): 648-657.
42. Koulman A., et al. “Peramine and other fungal alkaloids are exuded in the guttation fluid of endophyte infected grasses”. Phytochemistry 68 (2007): 355-360.
43. Lane GA., et al. Coevolution of fungal endophytes with grasses: The significance of secondary metabolites., Microbial endophytes, eds, New york, NY, USA, Marcel Dekker) (2000): 341-388.
44. Li GH., et al. “Nematicidal metabolites produced by the endophytic fungus Geotrichum sp”. Chemistry and Biodiversity 4 (2007): 1520-1524.
45. Maheshwari R. “What is an endophytic fungus?” Current Science 90 (2006): 1025.
46. Meir S., et al. “Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves”. Journal of Agricultural and Food Chemistry 43 (1995): 1813-1819.
47. Nalini MS., et al. “Endophytic fungal diversity in medicinal plants of Western Ghats, India”. International Journal of Biodiversity (2014): 9.
48. Nouari Sadrati., et al. “Screening of antimicrobial and antioxidant secondary metabolites from endophytic fungi isolated from Wheat (Triticum durum)”. Journal of Plant Protection Research2 (2013): 128.
49. Panaccione DG. “Origins and significance of ergot alkaloid diversity in fungi”. FEMS Microbiology Letters 251 (2005): 9-17.
50. Panaccione DG., et al. “Effects of ergot alkaloids on food preference and satiety of rabbits as assessed with gene-knockout endophytes in perennial ryegrass”. Journal Agricultural Food Chemistry 54 (2006): 4582-4587.
51. Rao S., et al. “The Botanophila-Epichloe association in cultivated Festuca in Oregon: evidence of simple fungivory”. Entamologia Experimentalis Et Applicata 115 (2005): 427-433.
52. Robards K., et al. “Phenolic compounds and their role in oxidative processes in fruits”. Food Chemistry 66 (1999): 401-436.
53. Rodriguez RJ., et al. “Stress tolerance in plants via habitat-adapted symbiosis”. International Society of Microbial Ecology 2 (2008): 404-416.
54. Saikkonen K., et al. “The persistence of vertically transmitted fungi in grass population, Proceedings of the Royal Society-B”. BiologicalSciences, 269 (2002): 1397-1403.
55. Singleton VL., et al. “Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent”. Methods in Enzymology 29 (1999): 152-178.
56. Stierle A., et al. “Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific Yew”. Science 260 (1993): 214-216.
57. Strobel G and Daisy B. “Bioprospecting for microbial endophytes and their natural products”. Microbiology and Molecular Biology Reviews 67 (2003): 491-502.

Citation

Citation: Brindha Lakshmi., et al. "A Comparative Study on the Antioxidant Activity of Four Different Fungal Endophytes".Acta Scientific Microbiology 3.1 (2020): 33-41.

Copyright

Copyright: © 2020 Brindha Lakshmi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.