Mahamoudou Sanou^1,2*, Absétou Ky/Ba¹, Delwindé Albert Komi¹, Marius Nagalo⁶, Fidèle Bakiono⁵, Cyrille Bisseye⁷, Abdoul Salam Ouédraogo³, Ramata Ouédraogo^1,2 and Jacques Simporé

¹ University Ouaga I Pr Joseph KI-ZERBO, Ouagadougou, Burkina Faso
² Pediatric University Hospital Charles De Gaulle, Ouagadougou, Burkina Faso
³ University Hospital Souro-Sanou, Bobo-Dioulasso, Burkina Faso
⁴ Pietro Annigoni Biomolecular Research Center (CERBA), Department of Biochemistry-Microbiology, University of Ouaga I Prof Joseph Ki Zerbo, Burkina Faso
⁵ Armed Forces Health Service, Ouagadougou, Burkina Faso
⁶ Department of Hematology/Oncology, Mayo Clinic Scottsdale, AZ, USA
⁷ Laboratory of Cellular and Molecular Biology, University of Franceville, Franceville, Gabon

*Corresponding Author: Mahamoudou Sanou, University Ouaga I Pr Joseph KI-ZERBO, Ouagadougou, Burkina Faso.

Received: February 16, 2018; Published: April 14, 2018

DOI: 10.31080/ASMI.2018.01.0049

Citation: Mahamoudou Sanou. et al. “Double Speed Transfusion in Burkina Faso: Results of the Elisa 4^th Generation Tests Versus Determine™ HIV½ of Blood Donors Presumed to be Seroconverting at National Blood Transfusion Center”. Acta Scientific Microbiology 1.5 (2018).

Abstract

  Conflicting results are often observed at blood donors who used 4^th generation Elisa and the Determine™ HIV½ tests. This study aims to assess both techniques and determine the most reliable test for blood transfusion.

  This study was conducted at the National Blood Transfusion Center-Ouagadougou. This was a prospective study with descriptive purpose of the results of the two HIV diagnostic techniques over a period of five months from January 1 to May 31, 2014. It covered a total of 10028 samples of which 30 were discordant. A recall of 30 donors with discordant results to the Determine™ HIV ½ have given 4 positive and 26 negative results. Considering the results of the second Determine™ HIV ½ test, we finally retain the following results.

  Among 10028 samples analyzed, 110 were positive for the 4^th generation ELISA. Of these 110 positives, the 3^rd generation DETERMINE™ HIV test yielded 80 positives and 30 negatives. Recall of the 30 donors with the discordant results two months later gave 4 positive and 26 negative results to the 3^rd generation DETERMINE™ HIV test. The Determine™ 3rd Generation Test gives 0.04% false negative in blood donors. The 4^th generation ELISA gives 0.3% false positive in blood donors.

  Considering the results of the second DETERMINE HIV½ 3^rd generation test, we do not recommend the use of the DETERMINE™ HIV™ 3rd Generation Test alone for HIV screening in blood donors

Keywords: Evaluation; Determine™ HIV ½, Elisa 4th Generation; Discordant; Seroconversion; Blood Donors