Acta Scientific Microbiology

Research ArticleVolume 1 Issue 4

Exploring Anti- Klebsiella pneumoniae Activity of Probiotic Lactobacilli of Curd Origin

Shyamapada Mandal* and Debashis Halder

Laboratory of Microbiology and Experimental Medicine, Department of Zoology, University of Gour Banga, Malda, India

*Corresponding Author: Shyamapada Mandal, Profeesor, Laboratory of Microbiology and Experimental Medicine, Department of Zoology, University of Gour Banga, Malda, West Bengal, India. E-mail: samtropmed@gmail.com

Received: February 09, 2018; Published: March 21, 2018

DOI: 10.31080/ASMI.2018.01.0038

Citation: Shyamapada Mandal and Debashis Halder. “Exploring Anti- Klebsiella pneumoniae Activity of Probiotic Lactobacilli of Curd Origin”. Acta Scientific Microbiology 1.4 (2018).

Abstract

  The current research evaluates the bacterial growth inhibitory activity of indigenous probiotic lactobacilli and standard Lacto- bacillus strains against multidrug resistant (MDR) Klebsiella pneumoniae . The probiotic lactobacilli strains ( Lactobacillus animalis LMEM6, L. plantarum LMEM7, L. acidophilus LMEM8 and L. rhamnosus LMEM9) from curd samples, and Lactobacillus fermentum MTCC 9748 standard strain were tested against indicator bacterial pathogens (n = 2): K. pneumoniae B535 clinical and K. pneumoniae NTCC 703603 standard strains, following agar overlay and agar-well diffusion techniques. The indicator bacterial strains were tested as MDR against 6 or more antibiotics with MAR (multiple antibiotic index) 0.4 - 0.73. The ZDI (zone diameter of inhibition) values from the action of test lactobacilli strains ranged 13.00 ± 1.00 - 33.33 ± 1.53, following agar-overlay method, and 12.00 ± 1.00 - 22.00 ± 1.00 mm, following agar-well method, against MDR K. pneumoniae indicator strains. The “R” value of lactobacilli strains ranged 3.00 - 13.17 mm, while the bacteriocin activity, in terms of activity unit (AU/ml), ranged 155.60 - 293.33. The study suggests that the indigenous lactobacilli might play an important role in the protection of host against MDR K. pneumoniae infection, and such probiotic strains may beneficially be employed as biotherapeutic agents in partial replacement or adjunct to antibiotic therapy in the treatment of MDR K. pneumoniae infection.

Keywords: Probiotic Lactobacilli; MDR Klebsiella pneumoniae; Antagonistic Activity; Bacteriocin Activity; MAR Index

Bibliography

  1. Al-Madboly LA and Abdullah AK. “Potent antagonistic ac- tivity of Egyptian Lactobacillus plantarum against multire- sistant and virulent food-associated pathogens”. Frontiers in Microbiology 6 (2015): 347.
  2. Halder D., et al . “Indigenous probiotic lactobacillus isolates presenting antibiotic like activity against human patho- genic bacteria”. Biomedicines 5.2 (2017): E31.
  3. Paczosa MK., et al . “Klebsiella pneumoniae: going on the offense with a strong defense”. Microbiology and Molecular Biology Reviews 80.3 (2016): 629-661.
  4. Lebeaux D., et al . “Biofilm-related infections: bridging the gap between clinical management and fundamental as- pects of recalcitrance toward antibiotics”. Microbiology and Molecular Biology Reviews 78.3 (2014): 510-543.
  5. Halder D and Mandal S. “Antibacterial potentiality of com- mercially available probiotic lactobacilli and curd lacto- bacilli strains, alone and in combination, against human pathogenic bacteria”. Translational Biomedicine 7 (2016): 2.
  6. Halder D and Mandal S. “Curd lactobacilli with probiotic potentiality”. Translational Biomedicine 6.2 (2015): 1-6.
  7. Lagrafeuille R., et al . “Opposing effect of Lactobacillus on in vitro Klebsiella pneumoniae in biofilm and in an in vivo intestinal colonisation model”. Beneficial Microbes 9.1 (2017): 87-100.
  8. Al-Mathkhury., et al . “Inhibitory effect of lactobacilli fil - trate on Klebsiella pneumoniae Biofilm”. The Iraqi Post - graduate Medical Journal 11.1 (2012): 168-179.
  9. Clinical and Laboratory Standards Institute (CLSI): Per - formance standards for antimicrobial susceptibility test- ing (2011) 21st informational supplement M100S21. CLSI, Wayne, Pa (2011).
  10. Nandi S and Mandal S. “Bacteriological profiling of com - mercially available eye cosmetics and their antibiotic sus- ceptibility pattern”. Translational Biomedicine 7.3 (2016): 1-8.
  11. Shokryazdan P., et al . “Probiotic potential of Lactobacillus strains with antimicrobial activity against some human pathogenic strains”. BioMed Research International (2014).
  12. Carasi P., et al . “Safety characterization and antimicrobial properties of kefir-isolated Lactobacillus kefiri”. BioMed Research International (2014): 208974.
  13. Pisano MB., et al . “Preliminary evaluation of probiotic properties of Lactobacillus strains isolated from Sardinian dairy products”. BioMed Research International (2014): 286390.
  14. Iyapparaj P., et al . “Optimization of bacteriocin produc- tion by Lactobacillus sp. MSU3IR against shrimp bacterial pathogens”. Aquatic Biosystems 9.1 (2013): 12.
  15. Yu WL and Chuang YC. “Clinical features, diagnosis, and treatment of Klebsiella pneumoniae infection” (2018).
  16. Lu CH., et al . “Klebsiella pneumoniae meningitis: analysis on clinical features of thirty-two adult patients”. Chinese Medi- cal Journal 60.6 (1997): 296-302.
  17. Lin WP., et al . “The Antimicrobial Susceptibility of Klebsiella pneumoniae from community settings in Taiwan, a trend analysis”. Scientific Reports 6 (2016): 36280.
  18. Gupta N., et al . “Carbapenem-resistant Enterobacteriaceae: Epidemiology and prevention”. Clinical Infectious Diseases 53.1 (2011): 60-67.
  19. Collado MC., et al . “The impact of probiotic on gut health”. Current Drug Metabolism 10.1 (2009): 68-78.
  20. Corr SC., et al . “Understanding the mechanisms by which probiotics inhibit gastrointestinal pathogens”. Advance Food and Nutrition Research 56 (2009): 1-15.
  21. Sanchez B., et al . “Extracellular proteins secreted by probi- otic bacteria as mediators of effects that promote mucosa- bacteria interactions”. Microbiology 156.11 (2010) 3232- 3242.
  22. Vuotto C., et al . “Probiotics to counteract biofilm-associated infections: promising and conflicting data”. International Journal of Oral Science 6.4 (2014) 189-194.
  23. Saranya S and Hemashenpagam N. “Antagonistic activity and antibiotic sensitivity of Lactic acid bacteria from fer - mented dairy products”. Advances in Applied Science Re- search 2.4 (2011): 528-534.
  24. Kormin S., et al . “Bacteriocin-producing lactic acid bacteria isolated from traditional fermented food”. Malaysian Jour - nal of Medical Sciences 8.1(2001): 63-68.
  25. Shehata MG., et al . “Screening of isolated potential probiotic lactic acid bacteria for cholesterol lowering property and bile salt hydrolase activity”. Annals of Agricultural Sciences 61.1 (2016): 65-75.

Copyright: © 2018 Shyamapada Mandal and Debashis Halder. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Member In





News and Events

  • Submission Timeline
    Last date for submission of articles is January 31, 2019.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Papers of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of “Best Article of the Issue”.
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes the authors for submission of articles towards the upcoming issue of respective journals.
  • Contact US