Activity of Probiotic Lactobacilli of Curd Origin
Shyamapada Mandal* and Debashis Halder
Laboratory of Microbiology and Experimental Medicine, Department of Zoology, University of Gour Banga, Malda, India
*Corresponding Author: Shyamapada Mandal, Profeesor, Laboratory of Microbiology and Experimental Medicine, Department of
Zoology, University of Gour Banga, Malda, West Bengal, India. E-mail:
February 09, 2018; Published: March 21, 2018
The current research evaluates the bacterial growth inhibitory activity of indigenous probiotic lactobacilli and standard
strains against multidrug resistant (MDR)
. The probiotic lactobacilli strains (
LMEM9) from curd samples, and
MTCC 9748 standard strain were tested against indicator bacterial pathogens (n = 2):
B535 clinical and
NTCC 703603 standard strains, following agar overlay and agar-well diffusion techniques. The indicator bacterial strains were tested
as MDR against 6 or more antibiotics with MAR (multiple antibiotic index) 0.4 - 0.73. The ZDI (zone diameter of inhibition) values
from the action of test lactobacilli strains ranged 13.00 ± 1.00 - 33.33 ± 1.53, following agar-overlay method, and 12.00 ± 1.00 - 22.00
± 1.00 mm, following agar-well method, against MDR
indicator strains. The “R” value of lactobacilli strains ranged
3.00 - 13.17 mm, while the bacteriocin activity, in terms of activity unit (AU/ml), ranged 155.60 - 293.33. The study suggests that
the indigenous lactobacilli might play an important role in the protection of host against MDR
infection, and such
probiotic strains may beneficially be employed as biotherapeutic agents in partial replacement or adjunct to antibiotic therapy in the
treatment of MDR
Keywords: Probiotic Lactobacilli; MDR Klebsiella pneumoniae; Antagonistic Activity; Bacteriocin Activity; MAR Index
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