Shyamapada Mandal* and Debashis Halder

Laboratory of Microbiology and Experimental Medicine, Department of Zoology, University of Gour Banga, Malda, India

*Corresponding Author: Shyamapada Mandal, Profeesor, Laboratory of Microbiology and Experimental Medicine, Department of Zoology, University of Gour Banga, Malda, West Bengal, India. E-mail: samtropmed@gmail.com

Received: February 09, 2018; Published: March 21, 2018

DOI: 10.31080/ASMI.2018.01.0038

Citation: Shyamapada Mandal and Debashis Halder. “Exploring Anti- Klebsiella pneumoniae Activity of Probiotic Lactobacilli of Curd Origin”. Acta Scientific Microbiology 1.4 (2018).

Abstract

  The current research evaluates the bacterial growth inhibitory activity of indigenous probiotic lactobacilli and standard Lacto- bacillus strains against multidrug resistant (MDR) Klebsiella pneumoniae . The probiotic lactobacilli strains ( Lactobacillus animalis LMEM6, L. plantarum LMEM7, L. acidophilus LMEM8 and L. rhamnosus LMEM9) from curd samples, and Lactobacillus fermentum MTCC 9748 standard strain were tested against indicator bacterial pathogens (n = 2): K. pneumoniae B535 clinical and K. pneumoniae NTCC 703603 standard strains, following agar overlay and agar-well diffusion techniques. The indicator bacterial strains were tested as MDR against 6 or more antibiotics with MAR (multiple antibiotic index) 0.4 - 0.73. The ZDI (zone diameter of inhibition) values from the action of test lactobacilli strains ranged 13.00 ± 1.00 - 33.33 ± 1.53, following agar-overlay method, and 12.00 ± 1.00 - 22.00 ± 1.00 mm, following agar-well method, against MDR K. pneumoniae indicator strains. The “R” value of lactobacilli strains ranged 3.00 - 13.17 mm, while the bacteriocin activity, in terms of activity unit (AU/ml), ranged 155.60 - 293.33. The study suggests that the indigenous lactobacilli might play an important role in the protection of host against MDR K. pneumoniae infection, and such probiotic strains may beneficially be employed as biotherapeutic agents in partial replacement or adjunct to antibiotic therapy in the treatment of MDR K. pneumoniae infection.

Keywords: Probiotic Lactobacilli; MDR Klebsiella pneumoniae; Antagonistic Activity; Bacteriocin Activity; MAR Index

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