Kruti K Patel, Firdosh Shah and Mitesh Dwivedi*

C. G. Bhakta Institute of Biotechnology, Faculty of Science, Uka Tarsadia University, Gujarat, India

*Corresponding Author: Mitesh Dwivedi, C. G. Bhakta Institute of Biotechnology, Faculty of Science, Uka Tarsadia University, Gujarat, India.

Received: January 30, 2018; Published: March 07, 2018

DOI: 10.31080/ASMI.2018.01.0032

Citation: Mitesh Dwivedi., et al. “Exopolysaccharide (EPS) Producing Isolates from Sugarcane Field Soil and Antibacterial Activity of Extracted EPSs”. Acta Scientific Microbiology 1.4 (2018).

Abstract

  Microbial exopolysaccharides (EPSs) are reported to possess unique physiological activities including anti-microbial, anti-tumor, and anti-inflammatory. The antimicrobial activities of EPS could be used as a potential source for the development of antimicrobial drugs and may have various industrial, pharmaceutical and medical applications. The present study was aimed to isolate efficient EPS producing bacteria from sugarcane field soil and to evaluate the antibacterial activity of EPSs. Total 9 EPS producing isolates were obtained out of which, KPEP3 and KPEP4 were found to be higher producers of EPS. The isolates were able to grow at different pH but EPS production was higher at pH 6. The maximum carbohydrate content was found in KPEP3 and minimum protein content was found in KPEP4 suggestive of their probable use in medicine due to less immunogenicity. The evaluation of antibacterial activity of EPS suggested that all 4 test microorganisms ( E. coli, B. cereus, B. subtilis and V. cholerae ) showed sensitivity against EPSs but the sensitivity varied from one microorganism to another. The KPEP3 and KPEP4 EPSs were found to exert higher antibacterial activity on diverse test organisms. EPSs concentration of 7.5 mg/ml was found to be effective for antibacterial activity. The MIC values of EPSs suggested that they can be used at lower concentration to inhibit test organisms. The biochemical characterization indicated that these isolates may belong to genera: Bacillus and Pseudomonas . Further research is necessary to elucidate the composition and efficacy of the EPSs by using in vitro and in vivo animal model studies.

Keywords: Exopolysaccharides (EPSs); Bacterial EPS; Antibacterial Activity

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