The Inconsistences of Quantitative Real Time Polymerase Chain Reaction in
Ousman Bajinka*, Khalid A Abdelhalim and Guven Ozdemir
Department of Microbiology, Ege University, Izmir, Turkey
*Corresponding Author: Ousman Bajinka, Department of Microbiology, Ege University, Izmir, Turkey.
December 21, 2017; Published: January 23, 2018
The use of quantitative methods of real time polymerase chain reactions (PCR) in the amplification and sequencing of DNA and
RNA (cDNA) has been a breakthrough in the annals of molecular biology. Its efficiency overweight those of its molecular method
counterparts however, against the lights of its specificity and sensitivity there are numerous loopholes that needed to be looked into,
analyzed and hence amended for better efficient results. This review highlighted the most common pitfalls and elaborated more on
each of them while suggesting ways of improving so as to avert the menace. Articles and research papers were selected at random
while making preferences on the most recent papers. Among the inconsistences of quantitative real time PCR; contamination comes
in different forms, poor master mix selection and preparations, the impurity of nucleic acid, the cost involved, the inability to detect
non-viable from living cells and new emerging cells, poor storing of the specimens and the high technical know-how. In addition, is
sues regarding multiplex PCR, failure for proofreading and internal controls and the inefficiency of antimicrobial sensitivity tests. All
the above pitfalls are avoidable and with the increasing innovation, the future lays the hope of achieving the very best results.
Keywords: Quantitative Real Time PCR; Inconsistences; Efficiency and Reliability; Sensitivity and Specificity
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