Acta Scientific Microbiology

Review Article Volume 1 Issue 2

The Inconsistences of Quantitative Real Time Polymerase Chain Reaction in Diagnostics Microbiology

Ousman Bajinka*, Khalid A Abdelhalim and Guven Ozdemir

Department of Microbiology, Ege University, Izmir, Turkey

*Corresponding Author: Ousman Bajinka, Department of Microbiology, Ege University, Izmir, Turkey.

Received: December 21, 2017; Published: January 23, 2018

DOI: 10.31080/ASMI.2018.01.0016

Citation: Ousman Bajinka., et al. “Probiotic Lactobacilli in Microbiology Research”. Acta Scientific Microbiology 1.1 (2018).

Abstract

  The use of quantitative methods of real time polymerase chain reactions (PCR) in the amplification and sequencing of DNA and RNA (cDNA) has been a breakthrough in the annals of molecular biology. Its efficiency overweight those of its molecular method counterparts however, against the lights of its specificity and sensitivity there are numerous loopholes that needed to be looked into, analyzed and hence amended for better efficient results. This review highlighted the most common pitfalls and elaborated more on each of them while suggesting ways of improving so as to avert the menace. Articles and research papers were selected at random while making preferences on the most recent papers. Among the inconsistences of quantitative real time PCR; contamination comes in different forms, poor master mix selection and preparations, the impurity of nucleic acid, the cost involved, the inability to detect non-viable from living cells and new emerging cells, poor storing of the specimens and the high technical know-how. In addition, is - sues regarding multiplex PCR, failure for proofreading and internal controls and the inefficiency of antimicrobial sensitivity tests. All the above pitfalls are avoidable and with the increasing innovation, the future lays the hope of achieving the very best results.

Keywords: Quantitative Real Time PCR; Inconsistences; Efficiency and Reliability; Sensitivity and Specificity

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Copyright: © 2018 Ousman Bajinka., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



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