S Mastraftsi ^1,2 , S Sarrou ^1,2 , K Pantelidi ^1,2 , A Skoulakis ^1,2 , A Roussaki ³ and E Petinaki ^1,2 *

¹Department of Microbiology, University Hospital of Larissa, Larissa, Greece
²Department of Microbiology, Medical School, University of Thessaly, Larissa, Greece
³Department of Dermatology, Medical School, University of Thessaly, Larissa, Greece

*Corresponding Author: E Petinaki, Professor, Head of Department of Microbiology, Medical School, University of Thessaly, Biopolis, Larissa, Greece.

Received: December 21, 2017; Published: January 19, 2018

DOI: 10.31080/ASMI.2018.01.0015

Citation: E Petinaki., et al. “Antimicrobial Resistance and Molecular Characterization of Staphylococcus aureus Strains Causing Skin and Soft Tissue Infections in Greek Adults”. Acta Scientific Microbiology 1.2 (2018).

Abstract

  During 2016, a total of one hundred twenty three Staphylococcus aureus isolates were collected from adult-patients skin and soft tissue infections (SSTIs) in Thessaly, Central Greece. The isolates were tested for susceptibility to various antimicrobial agents and the presence of resistance and virulence determinants. Resistance rates to cefoxitin, fusidic acid, erythromycin, clindamycin, tetra- cycline, ofloxacin and gentamycin were 22%, 36.6%, 29.3%, 26.8%, 22%, 7.3% and 4.8% respectively, whereas, all were susceptible to vancomycin, teicoplanin, linezolid, mupirocin, trimethoprim-sulfamethoxazole and daptomycin. The presence of erm (A), tet (M) and aac(6’)-Ie-aph(2’’) genes were found in erythromycin/clindamycin, tetracycline and gentamicin-resistant isolates, respectively. Molecular characterization of isolates revealed the presence of two clones among MRSA (ST80-IV and ST225-II), while, among MSSA twelve different STs (ST1, 15, 45, 72, 7, 22, 728, 34, 59, 10, 398, 1153) were identified. The Panton-Valentine Leukocidin gene was de - tected in 39 strains, 18 MRSA which belonged to ST80, and 21 MSSA, which belonged to ST72 and ST728. The Toxic Shock Syndrome Toxin gene was found in ST34 MSSA. The results highlight the importance of both MRSA and MSSA as etiological agents in SSTIs, emphasizing the need for a close surveillance.

Keywords: Antimicrobial Resistance; Greece; Molecular Typing; Skin and Soft Tissue Infection; Staphylococcus aureus

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Copyright: © 2018 E Petinaki., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.