Oladunmoye MK¹, Akinmaye AS¹ and Oladosu TO²*

¹Department of Microbiology, Federal University of Technology, Akure, Ondo State, Nigeria
²Department of Biological Sciences, Bells University of Technology, Ota, Ogun State, Nigeria

*Corresponding Author: Oladosu TO, Department of Biological Sciences, Bells University of Technology, Ota, Ogun State, Nigeria.

Received: December 07, 2017; Published: January 09, 2018

DOI: 10.31080/ASMI.2018.01.0011

Citation: Oladunmoye MK.,et al. “Antimicrobial Activity of Abdominal Gland Extracts of African Weaver Ants ( Oecophylla longinoda ) on Isolated Microorganisms from Kola Nut Pods”. Acta Scientific Microbiology 1.2 (2018).

Abstract

  This research was aimed at evaluating the antimicrobial potency of abdominal extracts of a species of weaver ants ( Oecophylla longinoda ) on microorganisms found on kola nut pods. Ten (10) samples of five infected and five uninfected kola nut pods were got - ten from a farm in Ibule Community, Ondo State, Nigeria. Microorganisms were isolated from these samples using standard bacte- riological and mycological methods. These microorganisms were identified adopting a series of tests such as Gram staining, colonial morphology and biochemical characterization. The bacterial isolates obtained include Pseudomonas aeruginosa, Bacillus subtilis, Ba- cillus cereus, Bacillus polymyxa, Serratia marcescens, Klebsiella oxytoca and Aeromonas hydrophila . While fungal isolates were Rhizo- pus stolonifer, Aspergillus niger, Penicillium digitatum, Aspergillus flavus and Aspergillus fumigatus . Microbial isolates were subjected to antibiotic susceptibility testing by using Tetracycline (30 μg), Ofloxacin (30 μg), Gentamicin (20 μg), Chloramphenicol (30 μg), Augmentin (30 μg), Ceftriaxone (30 μg), Nitrofurantoin (300 μg), Cotrimoxazole (25 μg), Ciprofloxacin (10 μg) and Amoxicillin (30 μg), while the antifungal drugs used were Griseofulvin, Ketoconazole, Itraconazole and Clotrimazole. Pseudomonas aeruginosa was the most resistant bacterial isolate to commercial antibiotics, while it displayed a great level of susceptibility to the abdominal ex - tracts at the different concentrations tested. This research revealed that extracts from the abdominal region of Oecophylla longinoda possess antimicrobial activities beneficial for the protection of kola nut against pathogenic microorganisms.

Keywords: Oecophylla longinoda ; Kola Nut Pods; Abdominal Gland

Bibliography

1. Brickell C., et al. “The American Horticultural Society Encyclopedia of plants and flowers”. D.K. Publishing, New York (2002): 720.
2. Ndagi I., et al. “Potentials and Challenges of Kolanut Production in Niger State, Nigeria”. Scholarly Research Notices: Agronomy (2012): 492394.
3. Agbeniyi SO and Fawole B. “Effects of curing and pre-storage die treatments on mould of kolanuts”. European Journal of Food Research Technology 208 (1999): 447-449.
4. Evans DA and Vidhu VV. “Ethnoentomological values of Oecophylla smaragdina (Fabricius)”. Current Science 109.3 (2015): 572-579.
5. Fawole MO and Oso BA. “Laboratory manual of Microbiology”. 5^th edition (2004).
6. Clinical Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement. CLSI document M100-S24 Wayne, PA 34.1 (2014): 50-98.
7. Otuonye AH and Adedeji AR. “Evaluation of selected plant extracts for the control of the storage rot diseases of kola nuts”. Annual Report of the Cocoa Research Institute of Nigeria, Ibadan (2006): 29-30.
8. Atanda OO., et al. “The quality of Nigerian kola nuts”. African Journal of Food Science 5.17 (2011): 904-909.
9. Scales BS., et al. “Comparative genomics of Pseudomonas fluorescens subclade III strains from human lungs”. BMC Genomics 16 (2015): 1032-1041.

Copyright: © 2018 Oladunmoye MK., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.