Lisha Patel^1* and Marina Fernandes²

¹Post Graduate Student, Department of Conservative Dentistry and Endodontics, Goa Dental College and Hospital, India
²Professor and Head of the Department, Department of Conservative Dentistry and Endodontics, Goa Dental College and Hospital, India

*Corresponding Author: Lisha Patel, Post Graduate Student, Department of Conservative Dentistry and Endodontics, Goa Dental College and Hospital, India.

Received: June 22, 2026; Published: July 14, 2026

Background: Dental bonding to pulp chamber dentin remains challenging due to its high organic content, altered collagen structure, and susceptibility to enzymatic degradation. Although universal adhesives containing functional monomers have demonstrated reliable bonding performance, adjunctive pretreatment strategies are often proposed to further enhance bond durability. Evidence supporting their effectiveness when used with universal adhesives in self-etch mode is limited.

Aim: To evaluate the combined effects of quercetin primer and diode laser irradiation on the Microtensile bond strength (MTBS) of universal adhesives to pulp chamber dentin.

Methods and Material: A total of 32 extracted human third molars were selected for the study. The samples were allocated into 4 experimental groups (n = 8/group) based on pretreatment protocol with quercetin primer and Laser irradiation: Group I, Group II, Group III, and Group IV. Then samples were subjected to Microtensile bond strength testing and failure mode analysis.

Statistical Analysis: Data were analysed using One way ANOVA, followed by Tukey’s post hoc test for intergroup comparisons.

Results: The control group demonstrated highest mean MTBS (8.839 MPa) when compared to other groups. One-way ANOVA revealed a statistically significant difference among groups (p = 0.019). Tukey’s post hoc test showed a significant difference only between Group I and Group IV (p = 0.029). Failure mode analysis revealed predominantly adhesive and resin-dentin interfacial failures.

Conclusion: The universal adhesive alone achieved optimal bonding to pulp chamber dentin, while quercetin primer and diode laser irradiation did not improve Microtensile bond strength of a universal adhesive applied in self-etch mode.

Keywords: Dentin Bonding; Self-Etch; Tensile Strength; Dentin; Flavonoids; Diode Laser

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Citation

Citation: Lisha Patel and Marina Fernandes. “Effect of Quercetin Primer and Diode Laser Irradiation on Microtensile Bond Strength of Universal Adhesive to Pulp Chamber Dentin: An In Vitro Study". Acta Scientific Dental Sciences 10.8 (2026): 19-26.

Copyright

Copyright: © 2026 Lisha Patel and Marina Fernandes. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.