Mithlesh Solanki¹*, Gokul Sridharan¹, Sangeeta Patankar¹, Rohit Suresh², Sonali Waghmare¹ and Ruchi Chadak¹

¹Department of Oral Pathology and Microbiology, Dr. G. D. Pol Foundation YMT Dental College and Hospital, Navi Mumbai, India
²Department of Biochemistry, MUHS, India

*Corresponding Author: Mithlesh Solanki, Department of Oral Pathology and Microbiology, Dr. G. D. Pol Foundation YMT Dental College and Hospital, Navi Mumbai, India.

Received: January 16, 2025; Published: January 27, 2025

Abstract

Objective(s): D To evaluate the periodontopathic bacteria through anaerobic culture and 16S rRNA sequencing for its identification and to validate any underlying sub-species present, and to determine anti-bacterial susceptibility in chronic periodontitis.

Method(s): D Subgingival plaque was collected from chronic periodontitis patients (n=10) for anaerobic culture and species characterization using conventional analysis. Microbial samples were subjected to 16S rRNA sequencing followed by basic local alignment search tool for species identification. Antibiotic sensitivity tests were performed using disk diffusion method.

Result(s): D Porphyromonas gingivalis, Fusobacterium nucleatum, Prevotella spp and Peptostreptococcus micros were identified by culture. These microorganisms after validation by molecular sequencing, revealed two species of genus Prevotella namely, P.Intermedia and P.Nigrescens. Antibiotic sensitivity tests showed significant susceptibility to moxifloxacin, metronidazole, amoxicillin/clavulanic acid, tetracycline and highest resistance to clindamycin.

Conclusion(s): D 16S rRNA sequencing may aid in identification of phenotypically similar but molecularly variable bacterial species thereby providing insights on their role in pathogenesis and treatment outcome of chronic periodontitis.

Keywords: Periodontopathic Bacteria; Anaerobic Culture; 16S rRNA Sequencing

References

1. Saini Rajiv., et al. “Periodontitis, a true infection”. Journal of Global Infectious Diseases2 (2009): 149-150.
2. Nazir Muhammad Ashraf. “Prevalence of periodontal disease, its association with systemic diseases and prevention”. International Journal of Health Sciences2 (2017): 72-80.
3. Silva Nora., et al. “Host response mechanisms in periodontal diseases”. Journal of Applied Oral Science: Revista FOB3 (2015): 329-355.
4. Shaju Jacob P., et al. “Prevalence of periodontitis in the Indian population: A literature review”. Journal of Indian Society of Periodontology1 (2011): 29-34.
5. Paster BJ., et al. “Bacterial diversity in human subgingival plaque”. Journal of Bacteriology 183,12 (2001): 3770-83.
6. Riep Birgit., et al. “Are putative periodontal pathogens reliable diagnostic markers?”. Journal of Clinical Microbiology6 (2009): 1705-1711.
7. Abe Flávia Casale., et al. “Antimicrobial resistance of microorganisms present in periodontal diseases: A systematic review and meta-analysis”. Frontiers in Microbiology 13 (2022): 961986.
8. Na Hee Sam., et al. "Identification of Potential Oral Microbial Biomarkers for the Diagnosis of Periodontitis”. Journal of Clinical Medicine 9,5 (2020): 1549.
9. How Kah Yan., et al. "Porphyromonas gingivalis: An Overview of Periodontopathic Pathogen below the Gum Line”. Frontiers in Microbiology 7 (2016).
10. Llama-Palacios Arancha., et al. “Proteomic analysis of Fusobacterium nucleatum growth in biofilm versus planktonic state”. Molecular Oral Microbiology4 (2020): 168-180.
11. Avila-Campos Mario J and Gustavo Velasquez-Melendez. “Prevalence of putative periodontopathogens from periodontal patients and healthy subjects in Sao Paulo, SP, Brazil”. Revista do Instituto de Medicina Tropical de Sao Paulo1 (2002): 1-5.
12. Verner C., et al. “Carpegen real-time polymerase chain reaction vs. anaerobic culture for periodontal pathogen identification”. Oral Microbiology and Immunology6 (2006): 341-346.
13. Kotsilkov Kamen., et al. "Comparison of culture method and real-time PCR for detection of putative periodontopathogenic bacteria in deep periodontal pockets”. Biotechnology and Biotechnological Equipment5 (2015): 996-1002.
14. Socransky SS., et al. “Microbial complexes in subgingival plaque”. Journal of Clinical Periodontology2 (1998): 134-44.
15. Bostanci Nagihan and Georgios N Belibasakis. “Porphyromonas gingivalis: an invasive and evasive opportunistic oral pathogen”. FEMS Microbiology Letters1 (2012): 1-9.
16. Meyer DH., et al. “Models of invasion of enteric and periodontal pathogens into epithelial cells: a comparative analysis”. Critical Reviews in Oral Biology and Medicine: An Official Publication of the American Association of Oral Biologists4 (1997): 389-409.
17. Boutaga Khalil., et al. “Periodontal pathogens: a quantitative comparison of anaerobic culture and real-time PCR”. FEMS Immunology and Medical Microbiology2 (2005): 191-199.
18. Rams TE., et al. “Peptostreptococcus micros in human periodontitis”. Oral Microbiology and Immunology1 (1992): 1-6.
19. Karched Maribasappa., et al. “Proteomic analysis of the periodontal pathogen Prevotella intermedia secretomes in biofilm and planktonic lifestyles”. Scientific Reports1 (2022): 5636.
20. Yang John Jeongseok., et al. "16S Ribosomal RNA Identification of Prevotella nigrescensfrom a Case of Cellulitis”. Annals of Laboratory Medicine5 (2013): 379-382.

Citation

Citation: Mithlesh Solanki., et al. “Characterization and Antimicrobial Sensitivity of Periodontopathic Bacteria in Chronic Periodontitis". Acta Scientific Dental Sciences 9.2 (2025): 68-72.

Copyright

Copyright: © 2025 Mithlesh Solanki., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.