Acta Scientific Dental Sciences (ASDS)(ISSN: 2581-4893)

Research Article Volume 8 Issue 2

Early Evaluation of Minor Serous Gland Injuries Following Fractionated Radiotherapy for Head and Neck Cancer (Immunohistochemical Study)

Mashael S Al-Qahtani1, Sherif S Hassan1,2*, and Ibraheem K Bamaga1

11Department of Basic and Clinical Oral Sciences, Faculty of Dental Medicine, Umm Al-Qura University, Makkah, KSA
2Department of Oral Biology and Dental Anatomy, Faculty of Dentistry, Al-Azhar University, Egypt

*Corresponding Author: Sherif S Hassan, Department of Basic and Clinical Oral Sciences, Faculty of Dental Medicine, Umm Al-Qura University, Makkah, KSA.

Received: January 08, 2024; Published: January 22, 2024


Objectives: This study aimed to analyse the histopathological and immunohistochemical expression of proliferating cell nuclear antigen (PCNA) and cytokeratin 17 in serous acini of Von Ebner glands of the irradiated versus non irradiated rats for head and neck malignancy to provide a more detailed profile of the radiation injury of the head and neck region.

Methods: Twenty male adult albino rats were divided into two groups; control group received no radiation and irradiated group received a radiation dose of 5 Gy daily for five consecutive days with a total dose of 25 Gy using therapeutic X-ray beam. One month after the last dose of irradiation, rats were euthanized and the tongue was carefully dissected out, fixed, and stained with H&E for routine histopathological examinations. Immunohistochemical staining was performed using antibodies specific for the expression of both proliferating cell nuclear antigen (PCNA) and cytokeratin 17 (CK17).

Results: Histological findings confirmed that the parenchyma of Von Ebner's gland in the radiation-exposed group had glandular atrophy characterized by loss of gland architecture, degenerated acini, and dilatation of the ductal system. Furthermore, there is a predominance of the fibrous component with the presence of fatty degeneration within the glandular tissue. Expression of PCNA in control Von Ebner gland revealed negative to weak reactivity, whereas the irradiated group showed moderate to strong staining in the gland parenchyma. Expression of CK17 in irradiated Von Ebner glands revealed significant moderate to strong reactivity in the acinar and ductal cells compared with weak to mild reactivity in control. The staining expression of irradiated group was varied from diffuse to locally concentrated at the apical cell part.

Conclusion: The severity and prevalence of PCNA and CK17 in the irradiated group from our results suggest a pathological effect that interferes with saliva production and/or secretion leading to xerostomia. Also, the intensity of PCNA expression in that group could be a pathogenic factor in carcinogenesis rather than the healing process in the serous apocrine glands.

Keywords:Radiotherapy; Cytokeratin 17; PCNA; Fractionated; Von Ebner Glan


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Citation: Sherif S Hassan., et al. “Early Evaluation of Minor Serous Gland Injuries Following Fractionated Radiotherapy for Head and Neck Cancer (Immunohistochemical Study)".Acta Scientific Dental Sciences 8.2 (2024): 50-54.


Copyright: © 2024 Sherif S Hassan., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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