Role of Direct Current Electrical Stimulation in Dental Implant Osseointegration - A Pilot Study
Barin LM1*, Barcelos RCS3, Palma VM2, Pillusky FM2, Kantorski KZ2,4, Maciel RM2 and Danesi CC2,5
1Department of Dentistry, Federal University of Santa Catarina, Florianópolis, RS, Brazil
2Post-Graduation Program in Dental Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil
3Post-Graduation Program in Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil
4Department of Stomatology, Federal University of Santa Maria, Santa Maria, RS, Brazil
5Department of Pathology, Federal University of Santa Maria, Santa Maria, RS, Brazil
*Corresponding Author:Barin LM, Department of Dentistry, Federal University of Santa Catarina, Florianópolis, RS, Brazil.
Received: May 04, 2023; Published: May 27, 2023
This study evaluated the effect of antimicrobial photodynamic therapy (aPDT) using methylene blue (MB) solubilized in ethanol as an adjuvant in periodontal treatment. One hundred and twenty Wistar rats were randomly divided into five experimental groups: negative control (NC, no periodontitis, n = 15) and positive control (PC, with periodontitis, without any treatment, n = 15). The other three groups had periodontitis and were treated with scaling and root planing (SRP, n = 30); SRP + aPDT + MB solubilized in water (aPDT I, n = 30); SRP + aPDT + MB solubilized in ethanol (aPDT II, n = 30). Periodontitis was induced by placing a cotton ligature around mandibular right first molar of each animal. The ligature was removed after 7 days and the treatments were applied. At 7, 15 and 30 days rats were euthanized and bone and gingival tissues were collected for histomorphometric and immunohistochemical analysis. The SRP group showed a delay in relation to elimination of the inflammatory infiltrate; aPDTs groups showed absence of inflammatory infiltrate in 7 days; aPDT II showed absence of leukocyte infiltration at 30 days. The treated groups showed no difference and low staining in relation to VEGF, while PC group showed intense immunopositivity. The aPDT provided better responses to the periodontal tissue compared to SRP alone in short-term. aPDT II demonstrated greater repair capacity in short-term and especially in long-term. VEGF was very intense in periodontitis in PC group, showing a relation at the inflammatory degree and disease progress.
Keywords: Ethanol; Inflammation; Periodontal Diseases; Photosensitizing Agents; Therapeutics; Vascular Endothelial Growth Factor
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