Sona Satleghi¹, Zahra Yadegari², Azieh Shahbazi², Hassan Ali Shafiee³

¹Under Graduate Student, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
²Departments of Dental Biomaterials, Dental school, Shahid Beheshti University of Medical Sciences, Tehran, Iran
³Professor of Orthodontics, Shahid Beheshti University of Medical Sciences, Tehran, Iran

*Corresponding Author: Hassan Ali Shafiee, Professor of Orthodontics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Received: December 28, 2022; Published: January 11, 2023

Abstract

Objectives: This study aimed to compare the antimicrobial efficacy of Mass and non-fluoridated Oral B mouthwashes against Streptococcus salivarius (S. salivarius), Streptococcus sobrinus (S. sobrinus), and Streptococcus sanguinis (S. sanguinis).

Methodology: In this in vitro, experimental study, S. salivarius, S. sobrinus, and S. sanguinis were separately cultured on brain heart infusion (BHI) agar. The antimicrobial activity of Mass and Oral B Pro-Expert mouthwashes against the abovementioned microorganisms was evaluated by the agar well-diffusion test; 0.2% chlorhexidine (CHX) served as the positive control, and saline was used as the negative control. The diameter of the growth inhibition zones was measured. The minimum inhibitory concentration (MIC) of each mouthwash against each microorganism was determined by the micro broth dilution technique. The minimum bactericidal concentration (MBC) of the mouthwashes was also determined.

Results: The MIC of both mouthwashes was the same (2048/1) for S. sobrinus. The MIC of Mass for S. sanguinis (2048/1) and S. salivarius (1/512) was lower than that of Oral B (1/1024 and 1/256, respectively). The MBC of both mouthwashes was the same for all three microorganisms (1/256 for S. sobrinus, 1/1024 for S. sanguinis, and 1/256 for S. salivarius).

Conclusion: The antimicrobial efficacy of Mass was similar to that of Oral B Pro-Expert against S. salivarius, S. sobrinus, and S. sanguinis.

Keywords:Streptococcus salivarius; Streptococcus sobrinus; Mouthwashes

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Citation

Citation: Hassan Ali Shafiee., et al. “In vitro Comparative Antimicrobial Efficacy of Mass and Oral B Mouth Washes against Streptococcus salivarius, Streptococcus sobrinus, and Streptococcus sanguinis". Acta Scientific Dental Sciences 7.2 (2023): 113-118.

Copyright

Copyright: © 2023 Hassan Ali Shafiee., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.