Evaluation of the Effect of Casein Phosphopeptide-Amorphous Calcium Phosphate (Cpp-Acp) on Edta Treated Root Dentin Microhardness when used as Final Irrigant in Single Rooted Teeth - An Invitro Study

Majd Abedrabbo¹*, Heba El Asfouri² and Dina Ahmed Ali Morsy³

¹Master’s degree candidate, Department of Endodontics, Faculty of Dentistry, Cairo University, Egypt
²Associate Professor of Endodontics, Department of Endodontics, Faculty of Dentistry, Cairo University, Egypt
³Lecturer of Endodontics, Department of Endodontics, Faculty of Dentistry, Cairo University, Egypt

*Corresponding Author:Majd Abedrabbo, Master’s degree candidate, Department of Endodontics, Faculty of Dentistry, Cairo University, Egypt.

Received: December 22, 2022; Published: January 11, 2023

Abstract

Aim: The aim of this study was to evaluate the effect of irrigation with CPP-ACP solution on EDTA treated root dentin microhardness when used as a final irrigant in single rooted teeth.

Methodology: Twenty one extracted human single rooted teeth with single canals were used. All teeth were decoronated at 12mm length from the apex.

Complete chemo-mechanical preparation was done using NaOCl as conventional irrigation protocol. Teeth were randomly divided into 3 groups (n = 7 in each) according to the final irrigation protocol as follows: Group (A) final irrigation with 17% EDTA for 5 minutes followed by CPP-ACP remineralizing solution for 10 minutes, Group (B) final irrigation with 17% EDTA for 5 minutes, and Group (C) final irrigation with normal saline for 5 minutes. Each root specimen was cut horizontally at 3 different levels (coronal, middle, apical). Vicker’s Microhardness Testing was done at three different horizontal depths from the canal lumen (25μm, 50μm, 100μm).

Results: The results showed the highest values of microhardness for group A, followed by group C and B respectively. There was a significant difference in microhardness values between group A and the other groups at the middle third of the root, whereas the difference at coronal and apical thirds was insignificant. In addition, the values of microhardness increased significantly at deeper depths of dentin away from the canal lumen towards cementum in all groups.

Conclusion: Final irrigation with CPP-ACP remineralizing solution may improve mechanical properties of dentin including microhardness, especially after EDTA irrigation for smear layer removal.

Keywords:Smear Layer; Demineralization; Remineralization; Mechanical Properties; Microhardness; Endodontic Irrigation

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Citation

Citation: Majd Abedrabbo., et al. “Evaluation of the Effect of Casein Phosphopeptide-Amorphous Calcium Phosphate (Cpp-Acp) on Edta Treated Root Dentin Microhardness when used as Final Irrigant in Single Rooted Teeth - An Invitro Study".Acta Scientific Dental Sciences 7.2 (2023): 99-105.

Copyright

Copyright: © 2023 Majd Abedrabbo., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.