Siva SP*, GV Thatchani and Aroma Sadya Tirkey
Department of Oral Pathology and Microbiology, Kerala University of Health Sciences, India
*Corresponding Author: Siva SP, Department of Oral Pathology and Microbiology, Kerala University of Health Sciences, India.
Received: October 04, 2022; Published: November 07, 2022
Aim: To assess the effectiveness of REAP technique as a better chairside diagnostic procedure than the conventional PAP technique in oral exfoliative cytology.
Materials and Methods: A total of 100 smears were collected from 50 patients clinically diagnosed with potentially malignant disorders indicated for oral exfoliative cytology. Both sets of smears were stained, one using the conventional PAP method and the other using the REAP method. The two sets of smears were observed by two trained pathologists and evaluated for staining quality. The time for staining was also assessed. Over a 15-month observation period, the color stability of the smears stained by PAP and REAP were compared.
Statistical Analysis: Chi-square test was used to assess the significance of the difference between proportions of optimal staining between the two methods. Quantitative parameters between techniques were compared using the independent sample t-test.
Results: The staining quality of REAP smears was better than conventional PAP and was statistically significant (p < 0.001). The staining time of REAP is completed in 7 minutes whereas the PAP technique took 30 minutes. Both REAP and PAP smears retained their colour stability for 15 months.
Conclusion: REAP technique is a better chair-side diagnostic procedure than the conventional PAP technique in oral exfoliative cytology as it produces better staining quality and involves minimal time. The colour preservation of REAP stained smears is also excellent.
Keywords:Oral Exfoliative Cytology; REAP; Conventional PAP
Citation: Siva SP., et al. “Effectiveness of Rapid, Economic, Acetic Acid, Papanicolaou Stain (REAP) Over Conventional Papanicolaou Stain in Oral Exfoliative Cytology". Acta Scientific Dental Sciences 6.11 (2022): 90-96.
Copyright: © 2022 Siva SP., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.