Comparative Evaluation of Streptococcus mutans Biofilm Collected from Excessive Adhesive Flash (EAF) in Patients Undergoing Fixed Orthodontic Appliance Therapy Bonded with Two Different Adhesives
Kritika P Suroliya1*, Priyanka P Niranjane2, Ranjit H Kamble3,
Sunita S Shrivastav4, Saurabh H Shingnapurkar5 and Murtaza S
1Consulting Orthodontist, Suyog Dental Clinic, Mumbai, Maharashtra, India
2Reader, Department of Orthodontics, Sharad Pawar Dental College, Wardha,
3Professor and Head, Department of Orthodontics, Sharad Pawar Dental College,
Wardha, Maharashtra, India
4Professor and Dean of Examinations, Sharad Pawar Dental College, Wardha,
5Private Practitioner, Suyog Dental Clinic, Mumbai, Maharashtra, India
6Post-Graduate Student, Department of Prosthodontics, Yenepoya Dental College,
Mangalore, Karnataka, India
*Corresponding Author: Kritika P Suroliyaa, Consulting Orthodontist, Suyog Dental Clinic, Mumbai, Maharashtra, India.
Received: October 03, 2022; Published: October 18, 2022
Objective: To assess accumulation of Streptococcus Mutans (SM), in biofilm collected from Excessive Adhesive Flash (EAF) produced from two different orthodontic adhesive resins, in patients undertaking fixed appliance therapy.
Materials and Methods: A prospective interventional split mouth design was followed. 20 patients were segregated based on the resin used: Group 1: Right maxillary lateral incisor bonded with non- tooth coloured resin [adh 1: Transbond Plus]; Group 2: LeR maxillary lateral incisor bonded with tooth coloured resin [adh 2: Transbond XT]. Plaque was procured from EAF at these sites at 4 inter- vals: T0- before bonding; T1-1-month aRer bonding; T2-2 months aRer bonding; T3-3 months aRer bonding. Colonization of SM was assessed by colony count.
Results: There was a statistically significant surge in SM count, at T1 in both groups. The colony counts of SM were greater in Group 2. A subsequent decrease was seen in colony counts of both groups at T2 and T3, as mouth rinse was prescribed aRer T1.
Conclusions: The EAF formed from both adhesives, showed increased colonisation of SM at T1. The amount of SM on the EAF
generated from Transbond XT was greater in comparison to Transbond Plus.
Keywords: Excessive Adhesive Flash; Streptococcus Mutans; Orthodontic
- Jurišić S., et al. “Assessment of eﬃcacy of two chlorhexidine mouth rinses on oral hygiene and gingival health in adolescents wearing two types of orthodontic brackets”. International Journal of Dental Hygiene 2 (2018): e52-e57.
- Ho CSF., et al. “Streptococcus mutans forms xylitol-resistant bioﬁlm on excess adhesive ﬂash in novel ex-vivo orthodontic bracket model”. American Journal of Orthodontics and Dentofacial Orthopedics4 (2017): 669-677.
- Fard BK., et al. “Eﬀectiveness of Mouth Washes on Streptococci in Plaque around Orthodontic Appliances”. ISRN Dentistry 2011 (2011): 1-4.
- Saffari F., et al. “The effects of chlorhexidine and persica mouthwashes on colonization of Streptococcus mutans on fixed orthodontics O-rings”. Journal of Dentistry1 (2015): 54.
- Artun J and Brobakken BO. “Prevalence of carious white spots aRer orthodontic treatment with multibonded appliances”. European Journal of Orthodontics 4 (1986): 229-234.
- Armstrong D., et al. “Excess Adhesive Flash Upon Bracket Placement: A Typodont Study Comparing APC PLUS and Transbond XT”. The Angle Orthodontist 6 (2007): 1101-1108.
- Chapman JA., et al. “Risk factors for incidence and severity of white spot lesions during treatment with ﬁxed orthodontic appliances”. American Journal of Orthodontics and Dentofacial Orthopedics2 (2010): 188-194.
- Ahn S-J., et al. “Roles of Salivary Proteins in the Adherence of Oral Streptococci to Various Orthodontic Brackets”. Journal of Dental Research 6 (2002): 411-415.
- Lundström F and Krasse BO. “Caries incidence in orthodontic patients with high levels of Strep- tococcus mutans”. European Journal of Orthodontics 1 (1987): 117-121.
- Gwinnett AJ and Ceen “Plaque distribution on bonded brackets: A scanning microscope study”. American Journal of Orthodontics 75.6 (1979): 667-677.
- Weitman RT and Eames WB. “Plaque accumulation on composite surfaces aRer various ﬁnishing procedures”. The Journal of the American Dental Association 1 (1975): 101-106.
- Ahn S-J., et al. “Prevalence of cariogenic streptococci on incisor brackets detected by polymerase chain reaction”. American Journal of Orthodontics and Dentofacial Orthopedics6 (2007): 736-741.
- Bishara SE and Ostby “White Spot Lesions: Formation, Prevention, and Treatment”. Seminars in Orthodontics 14.3 (2008): 174-182.
- Richter AE., et al. “Incidence of caries lesions among patients treated with comprehensive orthodontics”. American Journal of Orthodontics and Dentofacial Orthopedics5 (2011): 657-664.
- Julien KC., et al. “Prevalence of white spot lesion formation during orthodontic treatment”. The Angle Orthodontist 4 (2013): 641-647.
- Raina R. “A Comparison of Antibacterial Eﬃcacy of 0.5% Sodium Fluoride Impregnated Miswak and Plain Miswak Sticks on Streprococcus mutans - A Randomized Controlled Trial”. Journal of Clinical and Diagnostic Research 2 (2017): ZC01.
- Naranjo AA., et al. “Changes in the subgingival microbiota and periodontal parameters before and 3 months aRer bracket placement”. American Journal of Orthodontics and Dentofacial Orthopedics 3 (2006): 275.e17-275.e22.
- Jung W-S., et al. “Quantitative analysis of changes in salivary mutans streptococci aRer orthodontic treatment”. American Journal of Orthodontics and Dentofacial Orthopedics5 (2014): 603-609.
- Arab S., et al. “Effect of fixed orthodontic treatment on salivary flow, pH and microbial count”. Journal of Dentistry (Tehran, Iran)1 (2016): 18.
- Lara-Carrillo E., et al. “Eﬀect of orthodontic treatment on saliva, plaque and the levels of Streptococcus mutans and Lactobacillus”. Medicina Oral, Patologia Oral, Cirugia Bucal (2010): e924-e929.
- Mota SM., et al. “Streptococcus mutans counts in plaque adjacent to orthodontic brackets bonded with resin-modiﬁed glass ionomer cement or resin- based composite”. Brazilian Oral Research 1 (2008): 55-60.
- Koga-Ito CY., et al. “Correlation among mutans streptococci counts, dental caries, and IgA to Streptococcus mutans in saliva”. Brazilian Oral Research 4 (2004): 350-355.
- Lee S-P., et al. “Surface Characteristics of Orthodontic Materials and Their Effects on Adhesion of Mutans streptococci”. The Angle Orthodontist 2 (2009): 353-360.
- Sukontapatipark “Bacterial colonization associated with ﬁxed orthodontic appliances. A scanning electron microscopy study”. European Journal of Orthodontics 23.5 (2001): 475-484.
- Zachrisson “A posttreatment evaluation of direct bonding in orthodontics”. American Journal of Orthodontics 71.2 (1977): 173-189.