Acta Scientific Dental Sciences (ASDS)(ISSN: 2581-4893)

Review Article Volume 6 Issue 11

Decalcification- The Path to Hard Tissue Visualization

Tompala Vinod Kumar1, Leela Lavanya Gadam1, Ogirala Smyrna1 and Divya Uppala2*

1Postgraduate Student, Department of Oral Pathology and Microbiology, GITAM Dental College and Hospital, Dr. NTR University of Health Sciences (NTRUHS), Vijayawada, Andhra Pradesh
2Professor, Department of Oral Pathology and Microbiology, GITAM Dental College and Hospital, Dr. NTR University of Health Sciences (NTRUHS), Vijayawada, Andhra Pradesh

*Corresponding Author: Divya Uppala, Professor, Department of Oral Pathology and Microbiology, GITAM Dental College and Hospital, Dr. NTR University of Health Sciences (NTRUHS), Vijayawada, Andhra Pradesh.

Received: September 14, 2022; Published: October 04, 2022

Abstract

The decalcification procedure is a process of completely removing the calcium salt from the bone and teeth which are mineralized tissues, and also some other calcified tissues. Through this procedure mineralized component is removed, and the physical hardness also gets soft. Mineral components from the dentin and cementum should be removed. By using a microscope, we can observe pulp, immature enamel, dentin, and cementum histologically. This review tries to encompass the various procedures which help in diagnosing pathological hard tissue lesions.

Keywords: Decalcifying Agents; Acids; Endpoint Decalcification; Fixatives; Buffers; Chelating Agents; Resins; Microscope

References

  1. Kapila S. “Driving the Mineral out Faster: Simple Modifications of the Decalcification Technique”. Journal of Clinical and Diagnostic Research (2015).
  2. Gupta S., et al. “Tooth decalcification using different decalcifying agents - A comparative study”. Journal of Oral and Maxillofacial Pathology3 (2021): 463.
  3. Miquelestorena-Standley E., et al. “Effect of decalcification protocols on immunohistochemistry and molecular analyses of bone samples”. Modern Pathology8 (2020): 1505-1517.
  4. HOWE P. “Decalcification of teeth and bones, and regeneration of bone through diet”. JAMA: The Journal of the American Medical Association19 (1922): 1565.
  5. Hillemann H and Lee C. “Organic Chelating Agents for Decalcification of Bones and Teeth”. Stain Technology6 (1953): 285-287.
  6. Choube A., et al. “Comparison of decalcifying agents and techniques for human dental tissues”. Biotechnic and AMP; Histochemistry2 (2018): 99-108.
  7. Ali S., et al. “Evaluating the Efficacy of Two Decalcifying Agents on Hard and Soft Tissues of Human Permanent Teeth”. Journal of Evolution of Medical and Dental Sciences31 (2021): 2396-2400.
  8. Rehan A., et al. “Comparison of microwave decalcification with conventional decalcification method by using different decalcifying agents”. International Journal of Research in Medical Sciences7 (2017): 3126.
  9. Verdenius H and Alma L. “A quantitative study of decalcification methods in histology”. Journal of Clinical Pathology3 (1958): 229-236.
  10. Inaas Mahamad Apandi N., et al. “Bone Decalcification for Histological Examination of Biopsy Specimen”. Acta Scientific Dental Sciences5 (2021): 132-135.
  11.  

Citation

Citation: Divya Uppala., et al. “Decalcification- The Path to Hard Tissue Visualization". Acta Scientific Dental Sciences 6.11 (2022): 07-14.

Copyright

Copyright: © 2022 Divya Uppala., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate30%
Acceptance to publication20-30 days
Impact Factor1.278

Indexed In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is September 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US









ff

© 2024 Acta Scientific, All rights reserved.