Arup Kumar Saha¹, Pranab Karmaker², Diti Rani Das³, Abu Hena Mostafa Kamal⁴, Ariful Haque⁵, ASM Rafiul Haque⁶ and Md Ekramul Haque⁷*

¹Professor, City Dental College, Dhaka, Bangladesh

*Corresponding Author: Md Ekramul Haque, Professor, Department of Pharmacy, University of Rajshahi, Bangladesh.

Received: July 12, 2022; Published: August 12, 2022

Abstract

Crude rectified spirit extract of fifteen (15) selected medicinal plants under investigations were screened against dental caries forming bacteria from the 24 patients of Rajshahi Medical College Hospital. Preliminary screening reveals that only eleven plant extracts showed moderate to strong activity against cariogenic bacteria. Second screening leads to select only three (3) plants for the highest activity against cariogenic bacteria. These are Allium sativum, Psidium guajava and Erythrina variegata.

 Chloroform fraction of the crude rectified extracts of the Allium sativum showed the highest activity against cariogenic bacteria Streptococcus mutans both at a concentration of 200 and 400 µg/ml. Column chromatographic separation of this fraction a mixture of two compounds with very close RF values were isolated and identified as a mixture of Allicin and Allin having very strong activity against S. mutans with a zone of inhibition 19 and 24 mm at a concentration of 200 and 400 µg/ml disc of the compound which is somehow stronger than the standard kanamycin (11 mm, 30 µg/disc). A compound PG-1 was isolated from ethyl acetate extract of P. guajava showed remarkable, activity against S. mutans both at a concentration of 200 and 400 µg/ml(zone of inhibition 18 and 24mm). The compound was identified as Pinfaencin by spectral analysis.

 β-Sitosterol was also isolated from the methanol fraction of rectified spirit extract of the plant E. variegate showed moderate activity against cariogenic bacteria Streptococcus mutans, both under similar concentrations.

Keywords: Dental Caries; Cariogenic Bacteria; Medicinal Plants; Permanent Teeth; Compounds

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Citation

Citation: Md Ekramul Haque., et al. “Study on the Dental Caries Prevention Using Traditional Medicinal Plants of Bangladesh". Acta Scientific Dental Sciences 6.6 (2022): 20-28.

Copyright

Copyright: © 2022 Md Ekramul Haque., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.