Acta Scientific Dental Sciences (ASDS)(ISSN: 2581-4893)

Review Article Volume 6 Issue 7

Invitro Assessment of Herbal Extracts in Periodontics Using Antibacterial, Anti-inflammatory, Immunomodulatory and Antioxidant Activities: An Insight

Liya Anil and KL Vandana*

Department of Periodontics, College of Dental Sciences, Davangere, India

*Corresponding Author: KL Vandana, Department of Periodontics, College of Dental Sciences, Davangere, India.

Received: May 30, 2022; Published: June 10, 2022

Abstract

Many bacteria, in particular have unpredictable susceptibilities to antimicrobial agents and their susceptibilities can be measured in vitro to help guide the selection of the most appropriate antimicrobial agent. The various in vitro analysis used for this purpose are antibacterial, anti-inflammatory, immuomodulatory and antioxidant activities. The objective of this paper is to provide a brief review on various invitro activities such as antibacterial, anti-inflammatory, immuomodulatory and antioxidant effect on few herbal extracts used in periodontics and also to evaluate the invitro activities of polyherbal gel (HiOra-SG) and oleozone gel.

Keywords: Jasminum; Syzgium; Neem Oil; Olive Oil; Anti-Inflammatory; Antibacterial; Immunomodulatory; Invitro; Periodontitis; Herbs

References

  1. Bauer AW., et al. “Antibiotic susceptibility testing by a standardized single disc method”. American Journal of Clinical Pathology 45 (1966): 493-496.
  2. Wiegand I., et al. “Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances”. Nature Protocols 3 (2008): 163-175.
  3. Andrews JM. “Determination of minimum inhibitory concentrations”. Journal of Antimicrobial Chemotherapy 48 (2001): 5-16.
  4. “Determination of minimum inhibitory concentrations (MICs) of antibacterial agents by broth dilution”. Clinical Microbiology and Infection 6 (2003): 509-515.
  5. Guengerich FP. "Mechanisms of drug toxicity and relevance to pharmaceutical development". Drug Metabolism and Pharmacokinetics 26 (2011): 3-14.
  6. Schentag JJ. “Antimicrobial action and pharmacokinetics/pharmacodynamics: the use of AUIC to improve efficacy and avoid resistance”. Journal of Chemotherapy 11 (1999): 426-439.
  7. Stratton CW., et al. “Correlation of serum bactericidal activity with antimicrobial agent level and minimal bactericidal concentration”. The Journal of Infectious Diseases 154 (1982): 160-168.
  8. Markus Mueller. “Issues in Pharmacokinetics and Pharmacodynamics of Anti-Infective Agents: Kill Curves versus MIC”. Antimicrobial Agents and Chemotherapy (2004): 369-377.
  9. Kleiner DE and Stetler-Stevenson WG. “Quantitative zymography: detection of picogram quantities of gelatinases”. Analytical Biochemistry 218 (1994): 325-329.
  10. Baniyash M. “Chronic inflammation, immunosuppression and cancer: new insights and outlook”. Seminars in Cancer Biology 16 (2006): 80-88.
  11. Upadhyay SN. “Immunomodulation”. (Narosha publishing house, New Delhi) (1997): 98-110.
  12. R Diab-Ladki., et al. “Decrease in the total antioxidant activity of saliva in patients with periodontal diseases”. Clinical Oral Investigations 7 (2003): 103-107.
  13. SC Kim., et al. “Antioxidant profile of whole saliva after scaling and root planing in periodontal disease”. Journal of Periodontal and Implant Science 40 (2010): 164-171.
  14. Damoulis PD and Hauschka PV. “Nitric oxide acts in conjugation with proinflammatory cytokines to promote cell death in osteoblasts”. Journal of Bone and Mineral Metabolism 12 (1997): 412-422.
  15. Hukkanen M., et al. “Cytokine stimulated expression of inducible nitric oxide synthase by mouse, rat and human osteoblast like cells and its functional role in osteoblast metabolic activity”. Endocrinology 136 (1995): 5445-5453.
  16. Mizushima Y., et al. “Mode of stabilizing action of no- steroid drugs on erythrocyte”. Biochemical Pharmacology 19 (1970): 227-234.
  17. Heendeniya SN., et al.In vitro investigation of antiinflammatory activity and evaluation of phytochemical profile of Syzygium caryophyllatum”. Journal of Pharmacognosy and Phytochemistry 7 (2018): 1759-1763.
  18. Opie EL. “On the relation of necrosis and inflammation to denaturation of proteins”. Journal of Experimental Medicine 115 (1962): 597-608.
  19. Haynes AP and Fletcher J. “Neutrophil function tests”. Baillière's Clinical Haematology 3 (1990): 871-887.
  20. Pulikottil SJ and Nath S. “Potential of clove of Syzygium aromaticum in development of a therapeutic agent for periodontal disease: A review”. South African Dental Journal 70 (2015): 108-115.
  21. Ambarish S Sindagi., et al.In-vitro antibacterial activity of neem, clove, and cinnamon against Actinobacillus sp., isolated from chronic periodontitis patients”. Biomedicine 40 (2020): 214-219. 
  22. Bachiega TF., et al. “Clove and eugenol in noncytotoxic concentrations exert immunomodulatory/anti-inflammatory action on cytokine production by murine macrophages”. Journal of Pharmacy and Pharmacology 64 (2014): 610-616. 
  23. Amani MD., et al. “Antioxidant, Antimicrobial Activities and Volatile Constituents of Clove Flower Buds Oil”. Journal of Essential Oil-Bearing Plants 15 (2012): 900-907.
  24. Umamaheswari M., et al. “Antiulcer and In vitro antioxidant activities of Jasminum grandiflorum L”. Journal of Ethnopharmacology 4 (2007): 464-470.
  25. Tadiwos Y., et al. “Analgesic and anti-inflammatory activities of 80% methanol root extract of Jasminum abyssinicum Hochst. ex. Dc. (Oleaceae) in mice”. Journal of Ethnopharmacology 202 (2017): 281-289. 
  26. Sengar N., et al. “Anti-inflammatory, analgesic and anti-pyretic activities of standardized root extract of Jasminum sambac”. Journal of Ethnopharmacology 3 (2015): 140-148.
  27. Gupta VK., et al. “Antimicrobial potential of Glycyrrhiza glabra roots”. Journal of Ethnopharmacology 116 (2008): 377-380.
  28. Choi EM. “The licorice root derived isoflavan glabridin increases the function of osteoblastic MC3T3-E1 cells”. Biochemical Pharmacology 70 (2005): 363-368.
  29. Fu Y., et al. “Antioxidant and anti-inflammatory activities of six flavonoids separated from licorice”. Food Chemistry 15 (2013): 1063-1071.
  30. Jacob Vaya., et al. “Antioxidant Constituents from Licorice Roots: Isolation, Structure Elucidation and Antioxidative Capacity Toward LDL Oxidation”. Free Radical Biology and Medicine 23 (1997): 302-313.
  31. Fong LO., et al. “Antioxidant potential of an aqueous leaf extract of Neem (Azadirachta Indica A. Juss)”. Revista Cubana de Plantas Medicinales 19 (2014): 205-207.
  32. Schumacher M., et al. “Anti-inflammatory, pro-apoptotic, and anti-proliferative effects of a methanolic neem (Azadirachta indica) leaf extract are mediated via modulation of the nuclear factor-κB pathway”. Genes and Nutrition 6 (2011): 149-160.
  33. Jagadeesh K., et al. “Anti-Inflammatory Effect of Azadirachta Indica (Neem) In Albino Rats-An Experimental Study”. IOSR Journal of Pharmacy 4 (2014): 34-38.
  34. Sateesh Chickkanayakanahalli Parashuramaiah. “Effect of Neem Leaf and Neem Twig Extract on Prevotella Intermedia and Fusobacterium Nucleatum: An Ex Vivo Study”. IOSR Journal of Dental and Medical Sciences 18 (2019): 45-52.
  35. Shakti N., et al. “Immunomodulatory effects of neem (azadirachta indica) oil”. International Immunopharmacology 14 (192): 1187-1193.
  36. Kanjoormana Aryan Manu and Girija Kuttan. “Immunomodulatory activities of Punarnavine, an alkaloid from Boerhaavia diffusa”. Immunopharmacology and Immunotoxicology 31 (2009): 377-387.
  37. SA Kalasker V. “Evaluation of anti-inflammatory effect of aqueous extract of Boerhaavia diffusa leaves in rats”. International Journal of Research in Health Sciences 2 (2014): 517-521.
  38. Shisode and Kareppa. “In-vitro antioxidant activity and phytochemical studies of boerhaavia diffusa linn. Roots”. IJPSR 2 (2011): 3171-3176.
  39. Manasa Hosamane., et al. “Evaluation of holy basil mouthwash as an adjunctive plaque control agent in a four-day plaque regrowth model”. Journal of Clinical and Experimental Dentistry 6 (2014): 491-496.
  40. Mallikarjun S., et al. “Antimicrobial efficacy of Tulsi leaf (Ocimum sanctum) extract on periodontal pathogens: An In vitro study”. Journal of Indian Society of Periodontology 20 (2016): 145-150.
  41. Mohammadmehdi Fani and Jamshid Kohante. “Inhibitory activity of Aloe vera gel on some clinically isolated cariogenic and periodontopathic bacteria”. Journal of Oral Science 54 (2012): 15-21.
  42. Giampiero Pietrocola., et al. “Evaluation of the antibacterial activity of a new ozonized olive oil against oral and periodontal pathogens”. Journal of Clinical and Experimental Dentistry 10 (2018): 1103-1108.
  43. Sigrun Eick., et al. “Effect of ozone on periodontopathogenic species-an In vitro study”. Clinical Oral Investigations 16 (2012): 537-544.
  44. Tatsuya Fukui., et al. “Antimicrobial Effects of Ozone Gel”. Journal of Hard Tissue Biology 23 (2014): 445-448.

Citation

Citation: Liya Anil and KL Vandana. “Invitro Assessment of Herbal Extracts in Periodontics Using Antibacterial, Anti-inflammatory, Immunomodulatory and Antioxidant Activities: An Insight". Acta Scientific Dental Sciences 6.7 (2022): 25-38.

Copyright

Copyright: © 2022 Liya Anil and KL Vandana. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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