Acta Scientific Dental Sciences (ASDS)(ISSN: 2581-4893)

Research Article Volume 6 Issue 6

Detection of JP2 and Non-JP2 Genotype Strains of Aggregatibacter Actinomycetemcomitans in Localized Aggressive Periodontitis Patients Among the Egyptian Population

Ibrahim Hammad Ibrahim1, Alaa Moustafa Attia1,2*, Mohamed Fouad Edrees1, Mohamed A. EL-Mokhtar1 and Heba Ali Hammad3

1Department of Oral Medicine, Periodontolgy, Oral Diagnosis, and Dental Radiology, Faculty of Dental Medicine Al-Azhar University (Assiut), Egypt.
2Department of Basic and Clinical Oral Sciences, Division of Periodontology, Faculty of Dentistry, Umm Al-Qura University, Makkah, Saudi Arabia and Al-Azhar University (Assiut), Egypt.
3Department Microbiology and Immunology Faculty of Medicine, Assiut University, Egyot

*Corresponding Author: Alaa Moustafa Attia, Department of Oral Medicine, Periodontolgy, Oral Diagnosis, and Dental Radiology, Faculty of Dental Medicine AL-Azhar University (Assiut), Egypt and Department of Basic and Clinical Oral Sciences, Division of Periodontology, Faculty of Dentistry, Umm Al-Qura University, Makkah, Saudi Arabia and Al-Azhar University (Assiut), Egypt.

Received: May 06, 2022; Published:

Abstract

Background: JP2 clone of Aggregatibacter actimycetemcomitans has been mainly recovered from adolescents living in northwestern Africa or African descends. A very strong association between this and Aggressive periodontitis (AgP) specifically its localized form was found among adolescents.

Objectives: The purpose of the current study to evaluate the prevalence of Aggregatibacter actimycetemcomitans (Aa) genotype strains and to determine the effect of presence of these strains on the severity of localized aggressive periodontitis (LAP) patients at base line presentation among the Egyptian population.

Materials and Methods: This study was done on sixty patients (54 females and 6 males) aged (9-26) years diagnosed clinically and radiographically as a LAP patient were selected from examination of 270 periodontitis patients. The presence of Aa (JP2 and non-JP2) strains in the collected microbiological samples were identified by conventional PCR. All clinical parameters including plaque index (PI), gingival index (GI), probing depth (PD) and clinical attachment level (CAL) were taken for all the patients at base line.

Results: This study found that: Aggregatibacter actinomycetemcomitans (JP2 and non-JP2) was identified by PCR in 20/60 patients of the total participants in this study. Presence of Aa specifically its JP2 strain was associated with a significant increase in the estimated clinical parameters, which appeared clinically in the form of more destructive LAP cases at base line presentation.

Conclusion: The identification of Aa in minority of cases excludes the role of Aa as a major bacterial risk factor in LAP. JP2 and non-JP2 strain infection at baseline diagnosis can be predictive of potentially periodontal destruction in LAP patients. Further research are needed to clarify the role of JP2 in both severity and outcomes of periodontal management in people of LAP.

Keywords: Aggregatibacter; Actinomycetemcomitans; JP2 Strain

References

  1. Sharn Pal Sandhu., et al. “Unilateral gingival fibromatosis with localized aggressive periodontitis (involving first molars): An unusual case report”. Journal of Indian Society of Periodontology 2 (2009): 109-113.
  2. Nissen L., et al. “Lactobacillus salivarius down-regulate Aggregatibacter actinomycetemcomitans exotoxins expression”. Annals of Microbiology 2 (2014): 611-617.
  3. Balashova, N., et al. “Aggregatibacter actinomycetemcomitans Leukotoxin Induces Cytosol Acidification in LFA 1 Expressing Immune Cells”. Molecular Oral Microbiology 1 (2016): 106-114.
  4. Stähli A., et al. “JP2 Genotype of Aggregatibacter actinomycetemcomitans in Caucasian Patients: A Presentation of Two Cases”. Pathogens3 (2020): 178-186.
  5. Yoshida A., et al. “Quantitative discrimination of Aggregatibacter actinomycetemcomitans highly leukotoxic JP2 clone from non-JP2 clones in diagnosis of aggressive periodontitis”. BMC Infectious Diseases 10 (2012): 253-263.
  6. Johansson A., et al. “Genetic Profiling of Aggregatibacter actinomycetemcomitansSerotype B Isolated from Periodontitis Patients Living in Sweden”. Pathogens3 (2019): 153.
  7. Teughels W., et al. “Guiding periodontal pocket recolonization: a proof of concept”. Journal of Dental Research 11 (2007): 1078-1082.
  8. Armitage G.“Development of a classification system for periodontal diseases and conditions”. Annals of Periodontology 12 (1999): 1-6. 
  9. Silness J and Lo¨e H. “Periodontal disease in pregnancy II. Correlation between oral hygiene and periodontal condition”. Acta Odontologica Scandinavica 2 (1964): 112-135.
  10. Lo¨e H and Silness J. “Periodontal disease in pregnancy I. Prevalence and severity”. Acta Odontologica Scandinavica 12 (1963): 533-551.
  11. Ramfjord S. “The periodontal disease index (PDI)”. Journal of Periodontology 6 (1967): 602-610.
  12. Jervøe-Storm PM., et al. “Comparison of curette and paper point sampling of subgingival bacteria as analyzed by real-time polymerase chain reaction”. Journal of Periodontology5 (2007): 909-917.
  13. Stelzel M and Flores-de-Jacoby L. “Topical metronidazole application compared with subgingival scaling. A clinical and microbiological study on recall patients”. Journal of Clinical Periodontology 1 (1996): 24-29.
  14. Orru G., et al. “Usefulness of real time PCR for the differentiation and quantification of 652 and JP2 Actinobacillus actinomycetemcomitans genotypes in dental plaque and saliva”. BMC Infectious Diseases 6 (2006): 98-108.
  15. Mombelli A., et al. “The microbiota of osseointegrated implants in patients with a history of periodontal disease”. Journal of Clinical Periodontology2 (1995): 124-130.
  16. Boutaga K., et al. “Comparison of subgingival bacterial sampling with oral lavage for detection and quantification of periodontal pathogens by real-time polymerase chain reaction”. Journal of Periodontology 1 (2007): 79-86.
  17. Jervøe-Storm PM., et al. “Comparison of culture and real-time PCR for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples”. Journal of Clinical Periodontology 7 (2005): 778-783.
  18. Slots J and Ting M. “Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in human periodontal disease: occurrence and treatment”. Periodontology 1 (1999): 82-121.
  19. Lopez NJ., et al. “Occurrence of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis and Prevotella intermedia in juvenile periodontitis”. Journal of Clinical Periodontology 2 (1996): 101-105.
  20. Chahboun H., et al. “Bacterial profile of aggressive periodontitis in Morocco: a cross sectional study”. BMC Oral Health2 (2015): 15-25.
  21. Haubek D., et al. “Early onset periodontitis in Morocco is associated with the highly leucotoxic clone of Actinobacillus actinomycetemcomitans”. Journal of Dental Research 6 (2001): 1580-1583.
  22. Bandhaya P., et al. “Aggregatibacter actinomycetemcomitans serotypes, the JP2 clone and cytolethal distending toxin genes in a Thai population”. Journal of Clinical Periodontology6 (2012): 519-525.
  23. Haubek D. “The highly leukotoxic JP2 clone of Aggregatibacter actinomycetemcomitans: evolutionary aspects, epidemiology and etiological role in aggressive periodontitis”. Supplement 130.9 (2010): 1-53.
  24. Cortelli JR., et al. “Prevalence of periodontal pathogens in Brazilians with aggressive or chronic periodontitis”. Journal of Clinical Periodontology8 (2005): 860-866.
  25. Haubek D and Johansson A. “Pathogenicity of the highly leukotoxic JP2 clone of Aggregatibacter actinomycetemcomitans and its geographic dissemination and role in aggressive periodontitis”. Journal of Oral Microbiology 8 (2014): 6-28.
  26. Haubek D., et al. “The highly leukotoxic JP2 clone of Actinobacillus actinomycetemcomitans and progression of periodontal attachment loss”. Journal of Dental Research 10 (2004): 767-770.
  27. Cortelli SC., et al. “Diminished treatment response of periodontally diseased patients infected with the JP2 clone of Aggregatibacter (Actinobacillus) actinomycetemcomitans”.Journal of Clinical Microbiology 7 (2009): 2018-2025.
  28. Pihlstrom BC., et al. “Periodontal diseases”. Lancet366 (2005) :1809-1820.
  29. Haubek D., et al. “Prevalence of JP2 and Non-JP2 Genotypes of Aggregatibacter actinomycetemcomitans and Oral Hygiene Practice of Kenyan Adolescents in Maasai Mara”. Pathogens 10.4 (2021): 488-497.

Citation

Citation: Alaa Moustafa Attia., et al. “Detection of JP2 and Non-JP2 Genotype Strains of Aggregatibacter Actinomycetemcomitans in Localized Aggressive Periodontitis Patients Among the Egyptian Population". Acta Scientific Dental Sciences 6.6 (2022): 00-00.

Copyright

Copyright: © 2022 Alaa Moustafa Attia., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate30%
Acceptance to publication20-30 days
Impact Factor1.278

Indexed In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is November 30, 2022.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of “Best Article of the Issue”.
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.
  • Contact US