Fabio Alessandro Simões¹*, Antonio Carlos Aloise² and Lydia Masako Ferreira³

¹DDS, MSc, Surgical Translational Graduate Program at UNIFESP, São Paulo, SP, Brazil
²DDS, MSc, PhD, UNIFESP, São Paulo, SP, Brazil
³MD, PhD, Head and Full Professor Plastic Surgery Division UNIFESP, Researcher CNPq 1A, Director Translational Surgery Graduate Program UNIFESP, São Paulo, SP, Brazil

*Corresponding Author: Fabio Alessandro Simões, DDS, MSc, Surgical Translational Graduate Program at UNIFESP, São Paulo, SP, Brazil.

Received: July 03, 2021; Published: : August 19, 2021

Citation: Fabio Alessandro Simões, et al. “Comparative Analyses of Dense and Porous Chitosan-Xanthan Membranes". Acta Scientific Dental Sciences 5.9 (2021): 81-88.

Abstract

Introduction: Polymeric materials are often used in tissue engineering to foster the growth and/or healing of the most varied types of tissues and organs.

Objective: To analyze the physicochemical properties of porous chitosan-xanthan membranes.

Method: Chitosan-xanthan membranes were prepared through complexation of the polysaccharides Chitosan (Ch) and Xanthan (Xn) in a 1:1 mass ratio solution. The compact membranes (Control Group - CG) were obtained by modeling the polysaccharide complex onto polystyrene plates. Porous membranes (Experimental Group - EG) were obtained by adding Pluronic^® F127 to the polysaccharide complexes immediately before modeling onto the polystyrene plates. The membranes were characterized by analyzing the morphology, thickness, absorption, and degradation rates in aqueous (H₂O) and 0.9% NaCl (SS) solutions, as well as the mechanical resistance (maximum stress and elongation at rupture) of the Control and Experimental groups.

Results: For the CG and EG, the thickness of wet membranes was 2.39 ± 0.27mm and 2.69 ± 0.46mm, respectively (p > 0.05). Regarding water and saline absorption capacity, CG displayed 70.13 ± 3.77g and 28.92 ± 0.55g, respectively, and the EG 28.72 ± 0.91g and 15.21 ± 0.59g, respectively (p < 0.05). The mass variation of CG membranes exposed to water and saline solution was 12.85 ± 0.41% and 8.79 ± 1.40%, respectively, and for EG 16.13 ± 0.19% and 25.06 ± 0.99%, respectively (p < 0.05). The maximum stress at rupture of the CG and EG membranes was 0.03 ± 0.01 MPa and 0.05 ± 0.03 MPa, respectively (p < 0.05). The elongation at rupture of CG and EG membranes was 54.02 ± 16.45% and 50.86 ± 11.94%, respectively.

Conclusion: Porous chitosan/xanthan membranes showed less absorption of water and saline solution, greater variation in mass and greater resistance to tearing, when compared to compact membranes.

Keywords: Chitosan; Xanthan; Tissue Engineering; Scaffolds; Artificial Membranes; Support Tissue

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Copyright: © 2021 Fabio Alessandro Simões, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.