Acta Scientific Dental Sciences (ISSN: 2581-4893)

Research Article Volume 5 Issue 1

Evaluation of the Effect of Enlargement of the Apical One Third by Protaper Universal Rotary Files System on Elimination of Enterococcus faecalis and Surface Irregularities in Single Canaled Premolars (A Comparative In-Vitro Study)

Mohammed Yasien Taher Alagha1*, Maged Mohamed Negm2 and Shaimaa Ismail Gawdat3

1Masters Student, Faculty of Dentistry, Cairo University, Egypt
2Professor of Endodontics, Faculty of Dentistry, Cairo University, Egypt
3Associate Professor of Endodontics, Faculty of Dentistry, Cairo University, Egypt

*Corresponding Author: Mohammed Yasien Taher Alagha, Masters Student, Faculty of Dentistry, Cairo University, Egypt.

Received: December 04, 2020; Published: December 29, 2020



Aim of the Work: The aim of the study was to evaluate the effect of enlargement of the apical one third of root canals; by using F4 in comparison to F3 Protaper files; on the amount of Enterococcus Faecalis and surface irregularities in the apical third.

Materials and Methods: 24 freshly extracted human single canalled premolars were obtained. The root lengths were standardized to 15 ± 1 mm. The root canals were instrumented up to #20 K-file till the apical constriction for standardization. Following root canal preparation and sterilization, all root canals were completely filled with 30µ E. faecalis suspension and incubated at 37°C for one week with refreshment every 48 hours. All teeth were sampled after bacterial injection and before instrumentation for examination of bacterial presence inside the canals. For the apical one third assessment, Scanning Electron Microscope "SEM" was used to examine the surface irregularities.

Results: The results concerning bacterial reduction revealed that Group II (F4), showed a significantly higher E. faecalis reduction than Group I (F3) (P = 0.025). However, none of the preparation sizes rendered a bacterial free root canal system. Topographic surface analysis revealed that group II (F4) had significantly lower surface irregularities in comparison to group I (F3) (P = 0.036).

Conclusion: Within the limitations of this study, it could be concluded that none of the root canal preparation sizes resulted in complete E. faecalis elimination. Bacterial reduction has a direct relation with the enlargement of the mechanical preparation. The larger the file size the more significant is the bacterial reduction. This result might be related to the significant reduction in surface irregularities.

Keywords: Enterococcus faecalis; Surface Irregularities; Scanning Electron Microscope; Colony Forming Unit



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Citation: Mohammed Yasien Taher Alagha., et al. “Evaluation of the Effect of Enlargement of the Apical One Third by Protaper Universal Rotary Files System on Elimination of Enterococcus faecalis and Surface Irregularities in Single Canaled Premolars (A Comparative In-Vitro Study)”. Acta Scientific Dental Sciences 5.1 (2021): 100-109.


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