Acta Scientific Dental Sciences (ISSN: 2581-4893)

Research Article Volume 5 Issue 1

A Retrospective Epidemiological Study of Panoramic, Periapical and Cephalometric Radiographic Errors in Dentistry

Moataz Gamal Konsouh1*, Moahammad Adel Ezzat Khairy2 and Maha Abdelsalam Elbaz3

1Master’s Degree Student, Faculty of Dentistry, Cairo University, Egypt
2Professor of Conservative Dentistry, Faculty of Dentistry, Cairo University, Egypt
3Associate Professor of Conservative Dentistry, Faculty of Dentistry, Cairo University, Egypt

*Corresponding Author: Moataz Gamal Konsouh, Master’s Degree Student, Faculty of Dentistry, Cairo University, Egypt.

Received: November 27, 2020; Published: December 16, 2020



Aim: This study was conducted to determine the effect of using mouthwash of propolis, clove oil and chlorhexidine versus chlorhexidine mouthwash on the risk assessment of patients with high caries risk.

Materials and Methods: A total of 64 patients were assigned in this study. Patients were randomly divided into two groups according to type of mouthwash (A) where group A1 patients used Chlorhexidine mouthwash as control group while group A2 patients used Chlorhexidine with propolis and clove oil mouthwash as test group with 32 patients in each group. Each group was further divided into two groups according to application frequency (T) where T1 represents patients using the mouthwash once daily for one week every month for 6 months and T2 where patients used the mouthwash twice daily for one week every month for 6 months. Cariogram assessment was performed for each patient at baseline, 3 months and 6 months to obtain the caries risk percentage for each patient at the different time intervals.

Results: In the control group; significant decrease in the caries risk percentage resulted after 6 months (48.8 ± 7.3) for the once daily application frequency. The same resulted for twice daily application frequency, significant decrease after 6 months (45.8 ± 5.6). In the test group, significant decrease after 6 Months (49.7 ± 6.7) for the once daily application frequency. The same resulted for twice daily, significant decrease after 6 months (46.3 ± 6.0).

Conclusion: Within the limitations of this study, it can be concluded that:

  • Adding propolis and clove oil to Chlorhexidine is not beneficial in caries prevention.
  • The addition of propolis and clove oil to Chlorhexidine did not affect the reduction of bacterial mutans count in the oral cavity to a clinical significance.
  • Adding propolis and clove oil did not prolong the antimicrobial effect of Chlorhexidine on mutans.
  • Chlorhexidine mouthwash should be used twice daily to have a substantial effect in reducing oral mutans count.

Keywords: Dental Caries; Caries Risk Assessment; Mouthwash; Chlorhexidine; Propolis; Clove Oil; Streptococcus mutans; Bacterial Count



  1. Kim MJ., et al. “Antimicrobial effect of Korean propolis against the mutans streptococci isolated from Korean”. Journal of Microbiology 1 (2011): 161-164.
  2. Duailibe SADC., et al. “Effect of a propolis extract on Streptococcus mutans counts in vivo”. Journal of Applied Oral Science 5 (2007): 420-423.
  3. Fure S. “Ten-Year Incidence of Tooth Loss and Dental Caries in Elderly Swedish Individuals”. Caries Research6 (2003): 462-469.
  4. Petersen PE and Yamamoto T. “Improving the oral health of older people: The approach of the WHO Global Oral Health Programme”. Community Dentistry and Oral Epidemiology2 (2005): 81-92.
  5. Suneja ES., et al. “An Overview of Caries Risk Assessment: Rationale, Risk Indicators, Risk Assessment Methods, and Risk‑based Caries Management Protocols”. Indian Journal of Dental Sciences 3 (2017): 210-214.
  6. Autio-Gold J. “The Role of Chlorhexidine in Caries Prevention”. Operative Dentistry 33 (2008): 710-716.
  7. Walsh T., et al. “Chlorhexidine treatment for the prevention of dental caries in children and adolescents”. Cochrane Database System Review 4 (2015).
  8. Marcucci MC. “Propolis: chemical composition, biological properties and therapeutic activity”. Apidologie 2 (1995): 83-99.
  9. Özan F., et al. “Effect of Mouthrinse Containing Propolis on Oral Microorganisms and Human Gingival Fibroblasts”. European Journal of Dentistry4 (2007): 195-201.
  10. Hegde KS., et al. “Effect of Propolis on Streptococcus mutans Counts: An in Vivo Study”. International Journal of Clinical Pediatric Dentistry 1 (2013): 22-25.
  11. Nzeako BC., et al. “Antimicrobial activities of clove and thyme extracts”. Sultan Qaboos University Medical Journal 1 (2006): 33-39.
  12. Sabiha Shaheen S., et al. “Antimicrobial efficacy of ten commercially available herbal dentifrices against specific oral microflora - in vitro study”. Journal of Clinical and Diagnostic Research4 (2015): 42-46.
  13. Kanth MR., et al. “Efficacy of specific plant products on dental caries causing microorganisms”. Journal of Clinical and Diagnostic Research12 (2016): ZM01-3.
  14. Featherstone JDB., et al. “A randomized clinical trial of anticaries therapies targeted according to risk assessment (caries management by risk assessment)”. Caries Research2 (2012): 118-129.
  15. Sen N., et al. “A comparative assessment of caries risk using cariogram among smokers and smokeless tobacco users in india - a cross-sectional study”. African Health Science4 (2018): 1046-1056.
  16. Netto CA., et al. “Effects of typified propolis on mutans streptococci and lactobacilli: a randomized clinical trial”. Brazilian Dental Science2 (2013): 31-36.
  17. Petersson HG., et al. “Evaluation of a computer program for caries risk assessment in schoolchildren”. Caries Research5 (2002): 327-340.
  18. Anna Y Alian., et al. “Assessment of Caries Risk in Elderly Patients Using the Cariogram Model”. Journal of the Canadian Dental Association (Tor). 72.5 (2006): 459-463.
  19. Rosenow EC. “Studies on Elective Localization Focal Infection with Special Reference to Oral Sepsis”. Journal of Dental Research3 (1919): 205-267.
  20. Da Silva ACB., et al. “Detection of oral streptococci in dental biofilm from caries-active and caries-free children”. Brazilian Journal of Microbiology4 (2008): 648-651.
  21. Hsu KLC., et al. “Variability of two plaque sampling methods in quantitation of streptococcus mutans”. Caries Research2 (2010): 160-164.
  22. Subramaniam P., et al. “Effect of pomegranate and aloe vera extract on streptococcus mutans: An in vitro study”. Dental Hypotheses3 (2012): 99-105.
  23. Maldupa I., et al. “Comparative analysis of CRT Buffer, GC saliva check buffer tests and laboratory titration to evaluate saliva buffering capacity”. Baltic Dental and Maxillofacial Journal 13.2 (2011): 55-61.
  24. Silness J and LöE H. “Periodontal disease in pregnancy. II. Correlation between oral hygiene and periodontal conditions”. Acta Odontologica Scandinavica 22 (1964): 121-135.
  25. Bardisi W., et al. “Cariogram and Caries Risk Assessment for Athletes”. Egyptian Dental Journal3 (2018): 1181-1187.
  26. Kulkarni V V and Damle SG. “Comparative evaluation of efficacy of sodium fluoride, chlorhexidine and triclosan mouth rinses in reducing the mutans streptococci count in saliva: an in vivo study”. Journal of Indian Society of Pedodontics and Preventive Dentistry 3 (2003): 98-104.
  27. Jeon JG., et al. “Natural products in caries research: Current (limited) knowledge, challenges and future perspective”. Caries Research 3 (2011): 243-263.
  28. Ohta M., et al. “Evaluation of the Proportion of Cariogenic Bacteria Associated with Dental Caries”. Epidemiology5 (2017): 1-6.
  29. Samaranayake L. “Essential Microbiology for Dentistry”. 4th Editio. New York: Churchil Livingstone (2011): 121-193.
  30. Marsh PD. “Microbiological Aspects of the Chemical Control of Plaque and Gingivitis”. Journal of Dental Research 7 (1992): 1431-1438.
  31. Takahashi N and Nyvad B. “The role of bacteria in the caries process: Ecological perspectives”. Journal of Dental Research3 (2011): 294-303.
  32. Gao X., et al. “Validity of caries risk assessment programmes in preschool children”. Journal of Dentistry9 (2013): 787-795.
  33. Dent THS. “Predicting the risk of coronary heart disease. I. The use of conventional risk markers”. Atherosclerosis2 (2010): 345-351.
  34. Gao X., et al. “Caries risk assessment programmes for Hong Kong children”. Hong Kong Medical Journal6 (2015): S42-46.
  35. Akca AE., et al. “The Comparative Evaluation of the Antimicrobial Effect of Propolis with Chlorhexidine against Oral Pathogens: An in Vitro Study”. Biomed Research International 2016 (2016).
  36. Marya CM., et al. “Comparison of antimicrobial efficacy of brazilian propolis with chlorhexidine and sodium fluoride against common oral pathogens: An In Vitro study”. Jundishapur Journal of Natural Pharmaceutical Products 2 (2015): 1-6.
  37. Lang NP and Grossman KR. “Optimal dosage of chlorhexidine digluconate in chemical plaque control when applied by the oral irrigator”. Journal of Clinical Periodontology 8 (1981): 189-202.
  38. Clavero J., et al. “Effects of 0.2% chlorhexidine spray applied once or twice daily on plaque accumulation and gingival inflammation in a geriatric population”. Journal of Clinical Periodontology9 (2003): 773-777.
  39. Mannaa A., et al. “Caries-risk profile variations after short-term use of 5000 ppm fluoride toothpaste”. Acta Odontologica Scandinavica3 (2014): 228-234.
  40. Karabekiroǧlu S and Ünlü N. “Effectiveness of Different Preventive Programs in Cariogram Parameters of Young Adults at High Caries Risk”. Hindawi International Journal of Dentistry 7189270 (2017): 1-10.


Citation: Moataz Gamal Konsouh., et al. “Using A Mouthwash Containing Propolis, Clove Oil and Chlorhexidine to Improve the Caries Risk of High-risk Patients: Randomized Clinical Trial”. Acta Scientific Dental Sciences 5.1 (2021): 77-92.


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