Abstract

Background: To evaluate the effectiveness of normal saline, 2% Chlorhexidine solution and Ozone oil on microorganisms in the root canal.

Materials and Methods: This study was carried out on 45 patients, out of which 60 anterior fractured teeth were selected which are asymptomatic, non-vital and necrotic in nature. Teeth were randomly divided into three groups of 20 each depending upon the type of irrigants used i.e. Saline, Chlorhexidine and Ozone. After access opening, two microbial samples were taken from each tooth: a) Pre- irrigation i.e. sample after pulp extirpation and before irrigation, b) Post-irrigation i.e. sample after irrigation, which were stored in sterile containers and immediately transferred to microbiological laboratory for incubation aerobically at 37°C for 24 hours. Then, the samples were streaked on blood agar culture media and incubated for 48 hours. After 48 hours, the colony forming units were counted using a colony counter. Statistical analysis was carried out using Kruskal-Wallis test, Wilcoxson signed rank test and Mann-Whitney test.

Results: On intra group comparison, highly significant differences in the colony forming units were found between pre- and post-irrigated samples for all the three irrigants used i.e. saline, chlorhexidine and ozone. On inter group comparison, statistical difference was found between saline (group 1) and chlorhexidine (group 2), saline (group 1) and Ozone oil (group 3), chlorhexidine (group 2) and Ozone oil (group 3).

Conclusion: The result of this study shows that Ozone oil irrigating solution can be used as an irrigating solution and its antibacterial action is effective when it is compared with 2% chlorhexidine and saline.

Keywords: Saline; Chlorhexidine; Ozone Oil; Non-Vital Teeth

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Citation

Citation: Sarvepalli Venkata Satish., et al. “A Comparative Evaluation of the Effectiveness of Saline, Chlorhexidine and Ozone Irrigating Solutions on Microorganisms in the Root Canal - An In Vivo Study". Acta Scientific Dental Sciences 4.10 (2020): 76-81.

Copyright

Copyright: © 2020 Sarvepalli Venkata Satish., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.