Acta Scientific Dental Sciences (ISSN: 2581-4893)

Research Article Volume 4 Issue 8

Role of Xp-Endo Finisher Agitation File with the Use of EDTA and Chitosan, on Smear Layer Removal by SEM and the Use of Flame Atomic Absorption Spectrometry to Quantify the Concentration of Calcium Ions: An In Vitro Study

Marwan (Mohammad Shafik) Ashour Seyaj1*, Salsabyl Mohamed Ibrahim2 and Shaimaa Ismail Gawdat3

1Masters Student, Faculty of Dentistry, Cairo University, Egypt
2Professor of Endodontics, Faculty of Dentistry, Cairo University, Egypt
3Associate Professor of Endodontics, Faculty of Dentistry, Cairo University, Egypt

*Corresponding Author: Marwan (Mohammad Shafik) Ashour Seyaj, Masters Student, Faculty of Dentistry, Cairo University, Egypt.

Received: May 21, 2020; Published: July 10, 2020



Aim of the work: The aim of this study was to evaluate the effectiveness of the XP-endo Finisher file on smear layer removal in straight root canals after biomechanical instrumentation in comparison to two different chelating solutions (EDTA and Chitosan) by scanning electron microscope and to quantify the concentration of calcium ions on surface of root dentin by using atomic absorption spectrophotometry with flame (AASF).

Materials and Methods: Twenty freshly extracted, permanent, mandibular and maxillary premolars were included in the study. The sound teeth were decoronated at the cemento-enamel junction using a fine tapered diamond stone with round end mounted on high speed hand-piece under water coolant. The root canal of each specimen was mechanically prepared using a BT-Race rotary system (FKG Dentaire) and then divided randomly into two groups, as follows: EDTA group (n = 10) XP-endo Finisher file; 5-mL 17% EDTA. Chitosan group (n = 10) XP-endo Finisher file; 5-mL 0.2% Chitosan. The roots were longitudinally split and specimens were fixed on metallic subs and examined using an environmental SEM for the presence of smear layer and the containers were forwarded for spectrometric determination of calcium ion concentration within the liquid.

Results: Representative SEM images for EDTA group showed efficient smear layer removal with the dentinal tubules open in apical, middle and coronal thirds. On the other hand, chitosan group showed efficient smear layer removal, with coronal and middle thirds having more open dentinal tubules than the apical third. The mean Ca level was 45.58 ± 12.56 in EDTA group which recorded the highest Ca concentration among samples and chelated more calcium ion than others with 69.9 mg/L while the mean Ca level was 23.36 ± 6.38 in Chitosan group which recorded the lowest Ca concentration among samples with 12.9 mg/L. This difference was statistically significant p < 0.001, being higher in EDTA group.

Conclusion: Irrigation with 0.2% chitosan solution is as effective in root canal cleaning as 17% EDTA, in terms of smear layer removal. Regardless of the irrigation protocol, the apical third retains more smear layer than the coronal and middle thirds. Irrigation with 0.2% chitosan solution chelated less calcium than 17% EDTA.

Keywords: Difficulties; Reintegration; Professional; Psychiatric Patients - Lomé (TOGO)



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Citation: Marwan (Mohammad Shafik) Ashour Seyaj., et al. “Role of Xp-Endo Finisher Agitation File with the Use of EDTA and Chitosan, on Smear Layer Removal by SEM and the Use of Flame Atomic Absorption Spectrometry to Quantify the Concentration of Calcium Ions: An In Vitro Study". Acta Scientific Dental Sciences 4.8 (2020): 11-19.


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