Abstract

Background: Designed proteins hold great promise as building blocks for synthetic circuits, one such designer protein-degron LOCKR, which is based on ‘latching orthogonal cage-key proteins’ (LOCKR) technology-is a biological switch that degrades a protein of interest in vivo via a genetically encoded peptide. The designed nature of degron LOCKR proteins enables modifications to tune feedback behavior. This work demonstrates the large and untapped potential of de novo design of proteins for generating tools that implement complex synthetic functionalities in cells for a myriad of applications.

Materials and Methods: With the Medline database, Cochrane collaboration and Medknow database taken as a source for authenticated scientific research data, 70 articles were selected having undergone randomized control trial. Out of these, 17 articles (studies) were chosen which met the criterion for meta-analysis.

Results and Conclusion: LOCKR can act as a molecular switch. Individual LOCKR proteins can also be connected to form circuits that induce intracellular changes in response to both internal and external stimuli. The ability to engineer feedback control into living cells represents an important milestone in biology. LOCKR’s potential applications are endless, including biotechnological and therapeutic applications that can change the vista of cellular signaling.

Keywords: LOCKR; De Novo; Molecular Switch; Feedback Control; Meta-Analysis

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Citation

Citation: Gaurav. “LOCKR - A De Novo Biological Switch for Cellular Feedback Control: A Meta-Analysis Research”. Acta Scientific Dental Sciences 4.4 (2020): 124-129.

Copyright

Copyright: © 2020 Gaurav. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.