Acta Scientific Dental Sciences (ISSN: 2581-4893)

Research Article Volume 4 Issue 4

Comparative Evaluation of Antimicrobial Efficacy of Oxum Solution and Other Endodontic Irrigants Against Enterococcus Faecalis - An In vitro Study

Sukhbir Kour*, Trishagni Chaudhury and Pradeep PR

Department of Conservative Dentistry and Endodontics, University/Organization M.R Ambedkar Dental College and Hospital Bangalore India

*Corresponding Author: Sukhbir Kour, Department of Conservative Dentistry and Endodontics, University/Organization M.R Ambedkar Dental College and Hospital Bangalore India.

Received: February 28, 2020; Published: March 03, 2020

×

Abstract

  This study was done to test the antimicrobial efficiency of four irrigating solutions such as chlorhexidine (CHX), sodium hypochlorite (NaOCl), Bio pure MTAD, Oxum, against the E. Faecalis microorganisms in root canals. Fifty freshly extracted single- rooted human teeth were selected. They were enlarged to a size 45 k - File (Maillefer). Instrumentation was followed by irrigation with 5 ml of above irrigating solutions for each file used. 20µl of the suspension was inoculated into each of root canals. After 48 hr of incubation at 37°C, microbial growth was verified, confirming the contamination of the root canals. After the instrumentation, the sampling was done. The specimens were incubated at 37°C for 7 days. Within the limitations of this study it was concluded that MTAD significantly reduced the number of E. Faecalis followed by chlorhexidine and sodium hypochlorite and least reduction was observed in oxum.

Keywords: Antimicrobial Irrigating Solutions; Chlorhexidine; E. Faecalis; MTAD; Oxum

×

References

  1. Kakehashi S., et al. “The effect of surgical exposure of dental pulps in germ-free and conventional laboratory rats”. Oral Surgery, Oral Medicine, Oral Pathology, and Oral Radiology 20 (1965): 340-349.
  2. Moller AJ., et al. “Influence on periapical tissues of indigenous oral bacteria and necrotic pulp tissue in monkeys”. Scandinavian Journal of Dental Research 89 (1981): 475-484.
  3. Sundqvist GK., et al. “Capacity of anaerobic bacteria from necrotic dental pulps to induce purulent infections”. Infection and Immunity 25 (1979): 685-693. 
  4. Vivacqua-Gomes N., et al. “Recovery of Enterococcus faecalis after single- or multiple-visit root canal treatments carried out in infected teeth ex vivo”. International Endodontic Journal 38 (2005): 697-704.
  5. Sundqvist G. “Taxonomy, ecology, and pathogenicity of the root canal flora”. Oral Surgery, Oral Medicine, Oral Pathology, and Oral Radiology 78 (1994): 522-530.
  6. Yang SE., et al. “Effect of smear layer and chlorhexidine treatment on the adhesion of Enterococcus faecalis to bovine dentin”. Journal of Endodontics 32 (2006): 663-667.
  7. Gomes BP., et al. “Microbiological examination of infected dental root canals”. Oral Microbiology and Immunology 19 (2004): 71-76.
  8. Siqueira JF Jr., et al. “Efficacy of instrumentation techniques and irrigation regimens in reducing the bacterial population within root canals”. Journal of Endodontics 28 (2002): 181-184.
  9. Jeansonne MJ and White RR. “A comparison of 2.0% chlorhexidine gluconate and 5.25% sodium hypochlorite as antimicrobial endodontic irrigants”. Journal of Endodontics 20.6 (1994): 276-278.
  10. Filho MT., et al. “Inflammatory response to different endodontic irrigation solutions”. International Endodontic Journal 35 (2002): 735-739.
  11. White RR., et al. “Residual antimicrobial activity after canal irrigation with chlorhexidine”. Journal of Endodontics 23 (1997): 229-231.
  12. Kapur V and Marwaha AK. “Evaluation of effect and comparison of superoxidised solution (oxum) v/s povidone iodine (betadine)”. Indian Journal of Surgery 73.1 (2011): 48-53.
  13. Srikumar GP., et al. “Comparison of the antibacterial efficiency of MTAD, 2.5% sodium hypochlorite and 2% chlorhexidine against Enterococcus faecalis-an ex vivo study”. Endodontology 24 (2012): 41-47.
  14. LM Lin., et al. “Factors associated with endodontic treatment failures”. Journal of Endodontics 18.12 (1992): 625-627.
  15. Torabinejad M., et al. “The antimicrobial effect of MTAD: an in vitro investigation”. Journal of Endodontics 29.6 (2003): 400-403.
  16. Sundqvist G., et al. “Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment”. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology 85.1 (1998): 86-93.
  17. Afzal A., et al. “Antimicrobial activity of various irrigants against E. faecalis biofilm: An in vitro study”. Journal of Interdisciplinary Dentistry 3.2 (2013): 103.
  18. Siqueira Jr JF., et al. “Evaluation of the effectiveness of sodium hypochlorite used with three irrigation methods in the elimination of Enterococcus faecalis from the root canal, In vitro”. International Endodontic Journal 30.4 (1997): 279-282.
  19. Paul J. “Recent trends in irrigation in endodontics”. International Journal of Medical Microbiology 3.12 (2014): 941-952.
  20. U Sjogren., et al. “Influence ¨ of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis”. International Endodontic Journal 30.5 (1997): 297-306.
  21. JF Siqueira Jr., et al. “Evaluation of the effectiveness of sodium hypochlorite used with three irrigation methods in the elimination of Enterococcus faecalis from the root canal, In vitro”. International Endodontic Journal 30.4 (1997): 279-282.
  22. BM Newberry., et al. “The antimicrobial effect of biopure MTAD on eight strains of Enterococcus faecalis: an In vitro investigation”. Journal of Endodontics 33.11 (2007): 1352-1354.
  23. JM Davis., et al. “An In vitro comparison of the antimicrobial effects of various endodontic medicaments on Enterococcus faecalis”. Journal of Endodontics 33.5 (2007):  567-569. 
  24. Z Mohammadi and S Shahriari. “Residual antibacterial activity of chlorhexidine and MTAD in human root dentin In vitro”.  Journal of Oral Science 50.1 (2008): 63-67.
  25. M Torabinejad., et al. “The antimicrobial effect of MTAD: an In vitro investigation”. Journal of Endodontics 29.6 (2003): 400-403.
  26. TR Dunavant., et al. “Comparative evaluation of endodontic irrigants against Enterococcus faecalis biofilms”. Journal of Endodontics 32.6 (2006): 527-531.
  27. Nagayoshi M., et al. “Antimicrobial effect of ozonated water on bacteria invading dentinal tubules”. Journal of Endodontics 30.11 (2004): 778-781.
  28. Middleton AM., et al. “Comparison of a solution of super-oxidized water (Sterilox®) with glutaraldehyde for the disinfection of bronchoscopes, contaminated in vitro with Mycobacterium tuberculosis and Mycobacterium avium-intracellulare in sputum”. Journal of Hospital Infection 45.4 (2000): 278-282.
  29. Varma K., et al. “A comparative evaluation of pH, surface tension and antimicrobial efficacy of oxum, vancomycin, doxorubicin drugs vs. sodium hypochlorite–an In vitro study”. International Poster Journal of Dentistry and Oral Medicine 13.2 (2001): 528.
×

Citation

Citation: Sukhbir Kour., et al. "Comparative Evaluation of Antimicrobial Efficacy of Oxum Solution and Other Endodontic Irrigants Against Enterococcus Faecalis - An In vitro Study”. Acta Scientific Dental Sciences 4.4 (2020): 02-08.




Metrics

Acceptance rate30%
Acceptance to publication20-30 days
Impact Factor1.278

Indexed In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is December 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"

Contact US









ff

© 2024 Acta Scientific, All rights reserved.