Acta Scientific Dental Sciences (ISSN: 2581-4893)

Research Article Volume 4 Issue 3

Antimicrobial Efficacy and the Ability to Dissolve Pulp Tissues of Bio pure MTAD and Sodium Hypochlorite

Hayam Y Hassan1*, Maged M Negm2, Salah Abdalla3 and Ahmad Fawzy4

1Associate Professor of Endodontic, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt
2Professor of Endodontic, Faculty of Oral and Dental Medicine, Cairo University, Giza, Egypt
3Professor of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
4Associate Professor of Operative Dentistry, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt

*Corresponding Author: Hayam Y Hassan, Associate Professor of Endodontic, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt.

Received: February 04, 2020; Published: February 21, 2020



The Aim: of this study is to compare the antimicrobial efficacy and the ability to dissolve pulp tissues of Bio pure MTAD and sodium hypochlorite as endodontic irrigants.

Methods: A total of 36 patients were divided into three groups of 12 teeth each according to the three different irrigating solutions used (Bio pure MTAD, 5.25% and 2.5% sodium hypochlorite). Cleaning and shaping were completed for every root canal using the corresponding irrigant and the root canal for every case was ready for obturation. The swabs were transferred aseptically into Eppendorf tube. Microbial growth of Enterococcus faecalis was verified by blackening of Bile Esculin Azide agar. Furthermore, thirty six sterile Wassermann tubes were divided into three groups according to the tested materials. A piece of immediately extirpated vital human pulp (2mm x 1mm) was placed in each tube. All tubes were incubated for 24 hours at 37 °C. Conditions of the pulp tissue were examined every hour by means of a magnifying lens. At the end of 24 hours the remaining tissues of any pulp specimen were examined under the Stereomicroscope. 

Results: Bio pure MTAD completely inhibited the growth of Enterococcus faecalis, however, sodium hypochlorite had poor antimicrobial efficiency against Enterococcus faecalis. Sodium hypochlorite dissolved extirpated pulp tissues completely; while Bio pure MTAD did not. 

Conclusions: combination of using Sodium hypochlorite during cleaning and shaping and Bio pure MTAD as a final reins is better.

Keywords: Bio pure MTAD; Enterococcus faecalis; Antimicrobial Efficiency; Sodium Hypochlorite



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Citation: Hayam Y Hassan., et al. “Antimicrobial Efficacy and the Ability to Dissolve Pulp Tissues of Bio pure MTAD and Sodium Hypochlorite”. Acta Scientific Dental Sciences 4.3 (2020): 67-72.


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