Acta Scientific Dental Sciences (ISSN: 2581-4893)

Research Article Volume 4 Issue 3

Antimicrobial Efficacy and the Ability to Dissolve Pulp Tissues of Bio pure MTAD and Sodium Hypochlorite

Hayam Y Hassan1*, Maged M Negm2, Salah Abdalla3 and Ahmad Fawzy4

1Associate Professor of Endodontic, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt
2Professor of Endodontic, Faculty of Oral and Dental Medicine, Cairo University, Giza, Egypt
3Professor of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
4Associate Professor of Operative Dentistry, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt

*Corresponding Author: Hayam Y Hassan, Associate Professor of Endodontic, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt.

Received: February 04, 2020; Published: February 21, 2020

×

Abstract

The Aim: of this study is to compare the antimicrobial efficacy and the ability to dissolve pulp tissues of Bio pure MTAD and sodium hypochlorite as endodontic irrigants.

Methods: A total of 36 patients were divided into three groups of 12 teeth each according to the three different irrigating solutions used (Bio pure MTAD, 5.25% and 2.5% sodium hypochlorite). Cleaning and shaping were completed for every root canal using the corresponding irrigant and the root canal for every case was ready for obturation. The swabs were transferred aseptically into Eppendorf tube. Microbial growth of Enterococcus faecalis was verified by blackening of Bile Esculin Azide agar. Furthermore, thirty six sterile Wassermann tubes were divided into three groups according to the tested materials. A piece of immediately extirpated vital human pulp (2mm x 1mm) was placed in each tube. All tubes were incubated for 24 hours at 37 °C. Conditions of the pulp tissue were examined every hour by means of a magnifying lens. At the end of 24 hours the remaining tissues of any pulp specimen were examined under the Stereomicroscope. 

Results: Bio pure MTAD completely inhibited the growth of Enterococcus faecalis, however, sodium hypochlorite had poor antimicrobial efficiency against Enterococcus faecalis. Sodium hypochlorite dissolved extirpated pulp tissues completely; while Bio pure MTAD did not. 

Conclusions: combination of using Sodium hypochlorite during cleaning and shaping and Bio pure MTAD as a final reins is better.

Keywords: Bio pure MTAD; Enterococcus faecalis; Antimicrobial Efficiency; Sodium Hypochlorite

×

References

  1. Siqueira JF., et al. “Actinomyces species, Streptococci, and Enterococcus faecalis in primary root canal infections”. Journal of Endodontics 28 (2002): 168-172.
  2. Sundqvist G. “Associations between microbial species in dental root canal infections”. Oral Microbiology and Immunology 7 (1992): 257-262.
  3. Nair PN and Schroeder HE. “Periapical actinomycosis”. Journal of Endodontics12 (1984): 567-570.
  4. Kishen A., et al. “Chair side sensor for rapid monitoring of Enterococcus faecalis activity”. Journal of Endodontics30 (2004): 872-875.
  5. Sundqvist G., et al. “Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative retreatment”. Oral Surgery, Oral Medicine, Oral Pathology, and Oral Radiology 85 (1998): 86-93.
  6. Haffajee AD., et al. “Clinical and microbiological features of subjects with adult periodontitis who responded poorly to scaling and root planing”. Journal of Clinical Periodontology 24 (1997): 767-776.
  7. Nagayoshi M., et al. “Antimicrobial effect of ozonated water on bacteria invading dentinal tubules”. Journal of Endodontics  30 (2004): 778- 781.
  8. Hassan HY and Negm AM. “A comparative study to evaluate the effect of different irrigating solutions on the smear layer”. Egyptian Dental Journal 64.1 (2018): 7-15. 
  9. Nara A., et al. “Comparative evaluation of antimicrobial efficacy of MTAD, 3% NaOCI and propolis against E Faecalis”. International Journal of Clinical Pediatric Dentistry 3.1 (2010): 21-25. 
  10. Shabahang S and Torabinejad M. “Effect of MTAD on Enterococcus faecalis – contaminated root canals of extracted human teeth”. Journal of Endodontics 29 (2003): 576-579.
  11. Mohammadi Z and Shahriari S. “Residual antibacterial activity of chlorhexidine and MTAD in human root dentin In vitro”. Journal of Oral Science 50 (2008): 63-67.
  12. Mohammadi Z., et al. “Antimicrobial effect of three new and two established root canal irrigation solutions”. General Dentistry (2012): 534-539. 
  13. Torabinejad M., et al. “The antimicrobial effect of MTAD: An in vitro investigation”. Journal of Endodontics29 (2003): 400-403.
  14. Malkhassian G., et al. “Antibacterial efficacy of MTAD final rinse and two percent chlorhexidine gel medication in teeth with apical periodontitis: a randomized double-blinded clinical trial”. Journal of Endodontics35 (2009): 1483-1490. 
  15. Neglia R., et al. “Comparative in vitro and ex vivo studies on the bactericidal activity of Tetraclean, a new generation endodontic irrigant, and sodium hypochlorite”. New Microbiologica 31 (2008): 57-65.
  16. Estrela C., et al. “Efficacy of sodium hypochlorite and chlorhexidine against Enterococcus faecalis”. Journal of Oral Science 16 (2008): 364-368.
  17. Tirali RE., et al. In vitro antimicrobial activity of several concentrations of NaOCl and Octenisept in elimination of endodontic pathogens”. Oral Surgery, Oral Medicine, Oral Pathology, and Oral Radiology 108 (2009): 117-120.
  18. Stoll R., et al. “The disinfecting effect of ozonized oxygen in an infected root canal: an In vitro study”. Quintessence International 39 (2008): 231-236.
  19. Meire MA., et al. “Effectiveness of different laser systems to kill Enterococcus faecalis in aqueous suspension and in an infected tooth model”. International Endodontic Journal 42 (2009): 351-359.
  20. Kuştarci A., et al. “Bactericidal effect of KTP laser irradiation against Enterococcus faecalis compared with gaseous ozone: an ex vivo study”. Oral Surgery, Oral Medicine, Oral Pathology, and Oral Radiology 107 (2009): 73-79.
  21. Giardino L., et al. “Comparative evaluation of antimicrobial efficacy of sodium hypochlorite, MTAD, and Tetraclean against Enterococcus faecalis biofilm”. Journal of Endodontics 33 (2007): 852-855.
  22. Shenoi PR., et al. In vitro evaluation of the antimicrobial efficacy of chitosan and other endodontic irrigants against Enterococcus faecalis”. General Dentistry 64 (2016): 60-63.
  23. Charlie KM., et al. “A scanning electron microscope evaluation of smear layer removal and antimicrobial action of mixture of tetracycline, acid and detergent, sodium hypochlorite, Ethylenediaminetetraacetic Acid, and Chlorhexidine Gluconate: An In vitro Study”. Journal of International Society of Preventive and Community Dentistry 8 (2018): 62-69. 
  24. Beltz RE., et al. “Quantitative analysis of the solubilizing action of MTAD, sodium hypochlorite, and EDTA on bovine pulp and dentin”. Journal of Endodontics29 (2003): 334-337.
  25. Morgan RW., et al. “The solvent effects of calcium hydroxide irrigating solution on bovine pulp tissue”. Journal of Endodontics17 (1991): 165-168.
  26. Yang SF., et al. “Anaerobic tissue-dissolving abilities of calcium hydroxide and sodium hypochlorite”. Journal of Endodontics21 (1995): 613-616.
  27. Naenni N., et al. “Soft tissue dissolution capacity of currently used and potential endodontic irrigants”. Journal of Endodontics30 (2004): 785-787. 
  28. Spanó JC., et al. “Solvent action of sodium hypochlorite on bovine pulp and physico-chemical properties of resulting liquid”. Brazilian Dental Journal  12 (2001): 154-157.
  29. Okino LA., et al. “Dissolution of pulp tissue by aqueous solution of chlorhexidine digluconate and chlorhexidine digluconate gel”. International Endodontic Journal 37 (2004): 38-41.
  30. Johal S., et al. “Comparison of the antimicrobial efficacy of 1.3%NaOCl/Biopure MTAD to 5.25% NaOCl/15% EDTA for root canal irrigation”. Journal of Endodontics33 (2007): 48-51.
×

Citation

Citation: Hayam Y Hassan., et al. “Antimicrobial Efficacy and the Ability to Dissolve Pulp Tissues of Bio pure MTAD and Sodium Hypochlorite”. Acta Scientific Dental Sciences 4.3 (2020): 67-72.




Metrics

Acceptance rate30%
Acceptance to publication20-30 days
Impact Factor1.278

Indexed In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is July 10, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US