Acta Scientific Dental Sciences (ISSN: 2581-4893)

Research Article Volume 4 Issue 2

Antimicrobial Efficiency of Biopure MTAD and Sodium Hypochlorite as Endodontic Irrigants

Hayam Y Hassan1*, Maged M Negm2, Salah Abdalla3 and Ahmad Fawzy4

1Associate Professor of Endodontic, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt
2Professor of Endodontic, Faculty of Oral and Dental Medicine, Cairo University, Giza, Egypt
3Professor of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
4Associate Professor of Operative Dentistry, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt

*Corresponding Author: Hayam Y. Hassan, Associate Professor of Endodontic, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt

Received: January 09, 2020; Published: February 20, 2020

×

Abstract

The Aim: of this in vitro study is to compare the antimicrobial efficiency of Biopure MTAD and its effect together with sodium hypochlorite and hydrogen peroxide on Enterococcus faecalis

Methods: One hundred micro-liters of broth inoculated with Enterococcus faecalis was spread on sterile Mueller-Hinton agar (MHA) with sheep blood for each in six sterile glass plates. Approximately fifty micro-liters of each freshly prepared tested material were added onto the filter papers in the prepared plates. The plates were incubated at 37C for 48 hours and the zones of inhibition were measured. Furthermore, a total of 55 standardized root canals length and diameter were sterilized. Five teeth cultured to act as negative control (-ve). For infection of the root canals, a trypticase soy broth inoculated with Enterococcus faecalis was used. The roots incubated for 48 hours. Five teeth cultured to act as positive control (+ve). The remaining teeth were divided into three groups M, C and D (n = 15). Group M was irrigated with Bio pure MTAD. Groups C and D were irrigated with (5.25%) and (2.5%) sodium hypochlorite. Two or three sterile paper points were inserted into every root canal, and then transferred aseptically into its markedly corresponding Wassermann tube for each root. All the marked Wassermann tubes were incubated at 37C for 48 hours then microbial growth of Enterococcus faecalis was verified by turbidity of the broth. 

Results: Bio pure MTAD recorded the best results in respect of inhibiting the growth of Enterococcus faecalis. Moreover, additives as (1.3%) sodium hypochlorite and (20%) hydrogen peroxide decreased the efficiency of Bio pure MTAD against Enterococcus faecalis

Conclusions: Bio pure MTAD completely inhibited the growth of Enterococcus faecalis. Moreover sodium hypochlorite had poor antimicrobial efficiency against Enterococcus faecalis

Keywords: Biopure MTAD; Enterococcus faecalis; Antimicrobial Efficiency; Sodium Hypochlorite

×

References

  1. Siqueira JF., et al. “Actinomyces species, Streptococci, and Enterococcus faecalis in primary root canal infections”. Journal of Endodontics 28 (2002): 168-172.
  2. Sundqvist G. “Associations between microbial species in dental root canal infections”. Oral Microbiology and Immunology 7 (1992): 257-262.
  3. Nair PN and Schroeder HE. “Periapical actinomycosis”. Journal of Endodontics 12 (1984): 567-570.
  4. Kishen A., et al. “Chair side sensor for rapid monitoring of Enterococcus faecalis activity”. Journal of Endodontics 30 (2004): 872-875.
  5. Sundqvist G., et al. “Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative retreatment”. Oral Surgery, Oral Medicine, Oral Pathology, and Oral Radiology 85 (1998): 86-93.
  6. Haffajee AD., et al. “Clinical and microbiological features of subjects with adult periodontitis who responded poorly to scaling and root planning”. Journal of Clinical Periodontology 24 (1997): 767-776.
  7. Nagayoshi M., et al. “Antimicrobial effect of ozonated water on bacteria invading dentinal tubules”. Journal of Endodontics 30 (2004): 778- 781.
  8. Dang E. “Comparison of sodium hypochlorite and Chlorhexidine Gluoconate: Quality of current evidence”. Journal Youn Investment 19 (2008): 6-11.
  9. Giardino L., et al. “Comparative evaluation of antimicrobial efficacy of sodium hypochlorite, MTAD, and Tetraclean against Enterococcus faecalis biofilm”. Journal of Endodontics 33 (2007): 852-855.
  10. Hassan HY., et al. “Evaluation of antimicrobial efficacy of four tested solutions against Enterococcus faecalis, an In vitro study”. The Medical Journal of Cairo University 85.8 (2017): 3107-3112.
  11. Vianna ME and Gomes BP. “Efficacy of sodium hypochlorite combined with chlorhexidine against Enterococcus faecalis In vitro”. Oral Surgery, Oral Medicine, Oral Pathology, and Oral Radiology 107 (2009): 585-589.
  12. Johal S., et al. “Comparison of the antimicrobial efficacy of 1.3% NaOCl/BioPure MTAD to 5.25% NaOCl/15% EDTA for root canal irrigation”. Journal of Endodontics 33.1 (2007): 48-51. 
  13. Davis JM., et al. “An In vitro comparison of the antimicrobial effects of various endodontic medicaments on Enterococcus faecalis”. Journal of Endodontics 33 (2007): 567-569.
  14. Shabahang S and Torabinejad M. “Effect of MTAD on Enterococcus faecalis – contaminated root canals of extracted human teeth”. Journal of Endodontics 29 (2003): 576-579.
  15. Mohammadi Z and Shahriari S. “Residual antibacterial activity of chlorhexidine and MTAD in human root dentin In vitro”. Journal of Oral Science 50 (2008): 63-67.
  16. Shenoi PR., et al. “In vitro evaluation of the antimicrobial efficacy of chitosan and other endodontic irrigants against Enterococcus faecalis”. General Dentistry 64 (2016): 60-63.
  17. Torabinejad M., et al. “The antimicrobial effect of MTAD: An In vitro investigation”. Journal of Endodontics 29 (2003): 400-403.
  18. Charlie KM., et al. “A Scanning electron microscope evaluation of smear layer removal and antimicrobial action of mixture of tetracycline, acid and detergent, sodium hypochlorite, Ethylenediaminetetraacetic acid, and Chlorhexidine Gluconate: an In vitro study”. Journal of International Society of Preventive and Community Dentistry 8 (2018): 62-69. 
  19. Neglia R., et al. “Comparative in vitro and ex vivo studies on the bactericidal activity of Tetraclean, a new generation endodontic irrigant, and sodium hypochlorite”. New Microbiologica 31 (2008): 57-65.
  20. Estrela C., et al. “Efficacy of sodium hypochlorite and chlorhexidine against Enterococcus faecalis”. Journal of Oral Science 16 (2008): 364-368.
  21. Tirali RE., et al. “In vitro antimicrobial activity of several concentrations of NaOCl and Octenisept in elimination of endodontic pathogens”. Oral Surgery, Oral Medicine, Oral Pathology, and Oral Radiology 108 (2009): 117-120.
  22. Stoll R., et al. “The disinfecting effect of ozonized oxygen in an infected root canal: an In vitro study”. Quintessence International 39 (2008): 231-236.
  23. Meire MA., et al. “Effectiveness of different laser systems to kill Enterococcus faecalis in aqueous suspension and in an infected tooth model”. International Endodontic Journal 42 (2009): 351-359. 
  24. Kuştarci A., et al. “Bactericidal effect of KTP laser irradiation against Enterococcus faecalis compared with gaseous ozone: an ex vivo study”. Oral Surgery, Oral Medicine, Oral Pathology, and Oral Radiology 107 (2009): 73-79.
×

Citation

Citation: Hayam Y Hassan., et al. “Antimicrobial Efficiency of Biopure MTAD and Sodium Hypochlorite as Endodontic Irrigants”. Acta Scientific Dental Sciences 4.3 (2020): 47-51.




Metrics

Acceptance rate30%
Acceptance to publication20-30 days
Impact Factor1.278

Indexed In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is November 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US









ff

© 2024 Acta Scientific, All rights reserved.