Hayam Y Hassan1*, Maged M Negm2, Salah Abdalla3 and Ahmad Fawzy4
1Associate Professor of Endodontic, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt
2Professor of Endodontic, Faculty of Oral and Dental Medicine, Cairo University, Giza, Egypt
3Professor of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
4Associate Professor of Operative Dentistry, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt
*Corresponding Author: Hayam Y. Hassan, Associate Professor of Endodontic, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt
Received: January 09, 2020; Published: February 20, 2020
The Aim: of this in vitro study is to compare the antimicrobial efficiency of Biopure MTAD and its effect together with sodium hypochlorite and hydrogen peroxide on Enterococcus faecalis.
Methods: One hundred micro-liters of broth inoculated with Enterococcus faecalis was spread on sterile Mueller-Hinton agar (MHA) with sheep blood for each in six sterile glass plates. Approximately fifty micro-liters of each freshly prepared tested material were added onto the filter papers in the prepared plates. The plates were incubated at 37○C for 48 hours and the zones of inhibition were measured. Furthermore, a total of 55 standardized root canals length and diameter were sterilized. Five teeth cultured to act as negative control (-ve). For infection of the root canals, a trypticase soy broth inoculated with Enterococcus faecalis was used. The roots incubated for 48 hours. Five teeth cultured to act as positive control (+ve). The remaining teeth were divided into three groups M, C and D (n = 15). Group M was irrigated with Bio pure MTAD. Groups C and D were irrigated with (5.25%) and (2.5%) sodium hypochlorite. Two or three sterile paper points were inserted into every root canal, and then transferred aseptically into its markedly corresponding Wassermann tube for each root. All the marked Wassermann tubes were incubated at 37○C for 48 hours then microbial growth of Enterococcus faecalis was verified by turbidity of the broth.
Results: Bio pure MTAD recorded the best results in respect of inhibiting the growth of Enterococcus faecalis. Moreover, additives as (1.3%) sodium hypochlorite and (20%) hydrogen peroxide decreased the efficiency of Bio pure MTAD against Enterococcus faecalis.
Conclusions: Bio pure MTAD completely inhibited the growth of Enterococcus faecalis. Moreover sodium hypochlorite had poor antimicrobial efficiency against Enterococcus faecalis.
Keywords: Biopure MTAD; Enterococcus faecalis; Antimicrobial Efficiency; Sodium Hypochlorite
Citation: Hayam Y Hassan., et al. “Antimicrobial Efficiency of Biopure MTAD and Sodium Hypochlorite as Endodontic Irrigants”. Acta Scientific Dental Sciences 4.3 (2020): 47-51.
Copyright: © 2020 Hayam Y Hassan., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.