Acta Scientific Biotechnology

Research Article Volume 2 Issue 7

Efficient Immobilization of Milk Clotting Enzyme Produced by Rhizomucor miehei on Tricalcium Phosphate

Maysa Elsayed Moharam*, Magda A El- Bendary and Amira M Roshdy

Microbial Chemistry Department, Biotechnology Institute, National Research Centre, Elbohouth street, Dokki, Giza, Egypt

*Corresponding Author: Maysa Elsayed Moharam, Microbial Chemistry Department, Biotechnology Institute, National Research Centre, Elbohouth street, Dokki, Giza, Egypt.

Received: October 10, 2021; Published: October 23, 2021

Abstract

Milk clotting enzyme produced by Rhizomucor miehei NRRL 2034 under solid state fermentation was efficiently immobilized on Tricalcium phosphate (TCP) with immobilization degree of 90 % and activity retained of 93%. Optimum pH value for enzyme immobilization was pH 4. Stirring time of 30 min. was most suitable for the process at 30oC. 3 mg protein concentration was the best for ideal immobilization. Immobilized enzyme reacted optimally at pH 5 which represents the optimum value for the free enzyme preparation. Optimum temperature raised to 70°C for immobilized enzyme preparation in comparison to 60°C for free enzyme. Immobilized enzyme showed improved thermal stability.

 

Keywords: Milk Clotting Enzyme; Rhizomucor miehei; Immobilization; Tricalcium Phosphate

References

  1. Ahmed Samia A., et al. “Catalytic, kinetic and thermodynamic properties of free and immobilized caseinase on mica glass-ceramics”. Heliyon2019 (2019): e01674.
  2. Shieh CJ., et al. “Milk-clotting enzymes produced by culture of Bacillus subtilis natto”. Biochemical Engineering Journal 43 (2009): 85-91.
  3. Liburdi K., et al. “A preliminary study of continuous milk coagulation using Cynara cardunculus flower extract and calf rennet immobilized on magnetic particles”. Food Chemistry 239 (2018): 157-164.
  4. Jacob M., et al. “Recent advances in milk clotting enzyme”. The International Journal of Dairy Technology 64 (2011): 14-33.
  5. Lemes A., et al. “A new milk clotting enzyme produced by Bacillus spP45 applied in cream cheese development”. LWT- Food Science and Technology 66 (2016): 217-224.
  6. Foda MS., et al. “Over production of Milk Clotting Enzyme from Rhizomucor miehei through adjustment of growth under solid state fermentation conditions”. Australian Journal of Basic and Applied Sciences 8 (2012): 579-589.
  7. Mamo Jermen., et al. “Application of Milk-Clotting Protease from Aspergillus oryzae DRDFS13 MN726447 and Bacillus subtilis SMDFS 2B MN715837 for Danbo Cheese Production”. Journal of Food Quality (2020): 1-12.
  8. Vaysari Ak., et al. “Optimization of Mucor miehei Rennin Production and Recovery”. Scientia Irancia 1 (2002): 99-104.
  9. Benucci I., et al. “Pineapple stem bromelain immobilized on different supports: catalytic properties in model wine”. Biotechnology Progress 28 (2012): 1472-1477.
  10. Kim J., et al. “Nano biocatalysis and its potential applications”. Trends in Biotechnology 26 (2008): 639-646.
  11. Narwal SK., et al. “Green synthesis of isoamyl acetate via silica immobilized novel thermophilic lipase from Bacillus aerius”. Russian Journal of Bioorganic Chemistry 1 (2016): 69-73.
  12. Bonjour JP., et al. “Calcium-enriched foods and bone mass growth in prepubertal girls: a randomized, double-blind, placebo-controlled trial”. Journal of Clinical Investigation 6 (1997): 1287-1294.
  13. Richard C Ropp. “Encyclopedia of the Alkaline Earth Compounds”. Elsevier (2012).
  14. Abbas Hayam MMS., et al. “Production of White Soft Cheese Using Fungal Coagulant Produced by Solid State Fermentation”. World Applied Sciences Journal 6 (2013): 939-944.
  15. Greenberg DM. “Plant proteolytic enzymes”. in: Methods in Enzymology. Academic Press, New York 2 (1957): 54-59.
  16. Das G and Prabbu KA. “Immobilization of cane amylase and acid phosphatase on tricalcium phosphate (TCP)gel”. Enzyme and Microbial Technology 2 (1990): 625-630.
  17. Chae HJ., et al. “Improved immobilization yields by addition of protecting agent in glutaraldehyde induced immobilization of protease”. Journal of Bioscience and Bioengineering 89 (1998): 377-379.
  18. Han XO and Shahidi F. “Extraction of harp seal gastric proteases and their immobilization on chitin”. Food Chemistry 52 (1995): 71-76.
  19. El- Bendary Magda A., et al. “Efficient Immobilization of Milk Clloting Enzyme Produced by Bacillus sphaericus”. Polish Journal of Food and Nutrition Sciences11 (2009): 67-72.
  20. Esawy MA and Combet-Blanc Y. “Immobilization of Bacillus licheniformis 5A1 milk-clotting enzyme and characterization of its enzyme properties”. World Journal of Microbiology and Biotechnology 22 (2006): 197-200.
  21. Fox PF., et al. “Fundamentals of Cheese Science”. 2nd Library of Congress Control. ©Springer New York (2017).
  22. Vishwanta KS., et al. “Production and characterization of milk-clotting enzyme from Aspergillus oryzae MTCC 5341”. Applied Microbiology and Biotechnology 85 (2010): 1849-1859.
  23. “Preparation and characterization of alkaline protease immobilized on vermiculite”. Process Biochemistry 169 (1998): 173.
  24. Sahin SI., et al. “Purification, immobilization, and characterization of protease from local Bacillus subtilis M-11”. Asia-Pacific Journal of Chemical Engineering2015 (2015): 241-247.

Citation

Citation: Maysa Elsayed Moharam., et al. “Efficient Immobilization of Milk Clotting Enzyme Produced by Rhizomucor miehei on Tricalcium Phosphate". Acta Scientific Clinical Case Reports 2.7 (2021): 13-18.

Copyright

Copyright: © 2021 Maysa Elsayed Moharam., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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