Efficient Immobilization of Milk Clotting Enzyme Produced by Rhizomucor
miehei on Tricalcium Phosphate
Maysa Elsayed Moharam*, Magda A El- Bendary and Amira M Roshdy
Microbial Chemistry Department, Biotechnology Institute, National Research Centre, Elbohouth street, Dokki, Giza, Egypt
*Corresponding Author: Maysa Elsayed Moharam, Microbial Chemistry Department, Biotechnology Institute, National Research Centre, Elbohouth street, Dokki, Giza, Egypt.
October 10, 2021; Published: October 23, 2021
Milk clotting enzyme produced by Rhizomucor miehei NRRL 2034 under solid state fermentation was efficiently immobilized on Tricalcium phosphate (TCP) with immobilization degree of 90 % and activity retained of 93%. Optimum pH value for enzyme immobilization was pH 4. Stirring time of 30 min. was most suitable for the process at 30oC. 3 mg protein concentration was the best for ideal immobilization. Immobilized enzyme reacted optimally at pH 5 which represents the optimum value for the free enzyme preparation. Optimum temperature raised to 70°C for immobilized enzyme preparation in comparison to 60°C for free enzyme. Immobilized enzyme showed improved thermal stability.
Keywords: Milk Clotting Enzyme; Rhizomucor miehei; Immobilization; Tricalcium Phosphate
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