Acta Scientific Biotechnology

Research Article Volume 2 Issue 7

Efficient Immobilization of Milk Clotting Enzyme Produced by Rhizomucor miehei on Tricalcium Phosphate

Maysa Elsayed Moharam*, Magda A El- Bendary and Amira M Roshdy

Microbial Chemistry Department, Biotechnology Institute, National Research Centre, Elbohouth street, Dokki, Giza, Egypt

*Corresponding Author: Maysa Elsayed Moharam, Microbial Chemistry Department, Biotechnology Institute, National Research Centre, Elbohouth street, Dokki, Giza, Egypt.

Received: October 10, 2021; Published: October 23, 2021

Abstract

Milk clotting enzyme produced by Rhizomucor miehei NRRL 2034 under solid state fermentation was efficiently immobilized on Tricalcium phosphate (TCP) with immobilization degree of 90 % and activity retained of 93%. Optimum pH value for enzyme immobilization was pH 4. Stirring time of 30 min. was most suitable for the process at 30oC. 3 mg protein concentration was the best for ideal immobilization. Immobilized enzyme reacted optimally at pH 5 which represents the optimum value for the free enzyme preparation. Optimum temperature raised to 70°C for immobilized enzyme preparation in comparison to 60°C for free enzyme. Immobilized enzyme showed improved thermal stability.

 

Keywords: Milk Clotting Enzyme; Rhizomucor miehei; Immobilization; Tricalcium Phosphate

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Citation

Citation: Maysa Elsayed Moharam., et al. “Efficient Immobilization of Milk Clotting Enzyme Produced by Rhizomucor miehei on Tricalcium Phosphate". Acta Scientific Clinical Case Reports 2.7 (2021): 13-18.

Copyright

Copyright: © 2021 Maysa Elsayed Moharam., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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Impact Factor1.278

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