Acta Scientific ANATOMY

Research Article Volume 2 Issue 4

Nephroprotective Properties of Aqueous Extract of Ficus exasperata on Gentamicin-Induced Nephrotoxicity

Oviosun Augustine1*, Anyanwu Godson Emeka2, Oviosun Ezinne Chidinma3, Uchewa Onwe Obinna4, Obikili Emmanuel2 and Mayaki Esther Oluwatoyin1

1Department of Anatomy, Faculty of Basic Medical Sciences, Edo State University, Uzairue, Edo State, Nigeria
2Department of Anatomy, Faculty of Basic Medical Sciences, University of Nigeria, Enugu Campus, Enugu State, Nigeria
3Department of Anatomy, Faculty of Basic Medical Sciences, Ambrose Ali University, Ekpoma, Edo State, Nigeria
4Department of Anatomy, Faculty of Basic Medical Sciences, Alex Ekwueme Federal University, Ndufu Alike, Ebonyi State, Nigeria

*Corresponding Author: Oviosun Augustine, Department of Anatomy, Faculty of Basic Medical Sciences, Edo State University, Uzairue, Edo State, Nigeria.

Received: May 26, 2023; Published: June 27, 2022

Abstract

Background: Drug induced nephrotoxicity is a major public health challenge that is increasing across the globe. Ficus exasperata commonly known as sand paper leaf is used in traditional medicine for treatment of many diseases; hence this study is designed to investigate the nephroprotective effect of aqueous extract of Ficus exasperata on Gentamicin mediated nephrotoxicity.

Materials and method: Thirty (30) adult male wistar rats, weighing 160g-200g were used for this study. The rats were divided into six (6) groups of five (5) rats each (n = 5). The rats in group (A) served as control groups and received normal saline for 14 days. Rats in Group B were administered intraperitoneally with 100mg/kg of Gentamicin for fourteen (14) days. Group C and D were administered daily with aqueous extract of Ficus exasperata (100mg/kg and 200mg/kg respectively) for fourteen (14) days. Group E and F were treated with extract orally for 7 days and administered with Gentamicin and extract for next seven (7) days. On day 15th, the rats were weighed and sacrificed by cervical dislocation. The kidney was harvested for histological investigation. Blood samples were collected via cardiac puncture for biochemical analysis to ascertain serum creatinine, urea level and serum protein level. Data generated from this study were analyzed using SPSS, P-value less than 0.05 was considered as statistically significant.

Results: There was significantly high level of serum creatinine and blood nitrogen urea level in group B when compared to the normal control group, this indicates a great level of kidney damage as a result of gentamicin. There was significant reduction in creatinine, urea and protein level in all the groups treated with the ficus exasperata when compared to gentamicin only treated group. Histological sections of the kidney revealed tubular necrosis in the gentamicin only treated group. However, in all the groups treated with ficus exasperata extract the microanatomy of the kidney showed a normal kidney histology with no sign of tubular necrosis or inflammation of the glomeruli.

Conclusion: This study indicates that aqueous extract of Ficus exasperata leaf extract protects the kidney against Gentamicin induced nephrotoxicity. The protective potentials of ficus exasperata extract may be attributed to high concentration of alkaloids and flavonoids

Keywords: Ficus exasperata; Gentamicin; Nephrotoxicity; Serum Creatinine; Blood Nitrogen Urea Level

References

  1. Finn W and Porter G. Uniary Biomarkers and nephrotoxicity. Clinical Nephrotoxin. 2nd edition Kluveir. Academic Publishers Massachusetts (2003): 621-626.
  2. Awdishu L and Mehta RL. “The 6R’s of drug induced nephrotoxicity”. BMC Nephrology 18 (2007): 124.
  3. Schrier RW and Wang W. “Acute renal failure and sepsis”. The New England Journal of Medicine 2 (2004): 159-169.
  4. Schrier RW and Gottaschalk CW. “Disease of kidney. 5th 2nd published by little brown and co. (1993): 1031-1165.
  5. Joseph N., et al.“Effect of virgin coconut oil on the kidney of wistar rats exposed to a dose of paraquat”. Journal of Medical Review and Case Reports5 (2020): 136-140.
  6. Kim SY and Moon A. “Drug-induced nephrotoxicity and its biomarkers”. Biomolecules and Therapeutics (Seoul) 3 (2012): 268-272.
  7. Moulds R and Jeyasingham M. "Gentamicin: a great way to start". Australian Prescriber 33 (2012): 134-135.
  8. Martínez-Salgado., et al.“Glomerular nephrotoxicity of aminoglycosides”. Toxicology and Applied Pharmacology 1 (2007): 86-98.
  9. Souza VB., et al.“Alterações renais por aminoglicosídeos”. ARQ Medical Systems 4-5 (2008): 131-135.
  10. Abdel-Zaher., et al.“The potential protective role of alphalipoic acid against acetaminophen- induced hepatic and renal damage”. Toxicology 3 (2007): 261-270.
  11. Adekeye AO., et al.Ficus exasperata Vahl leaves extract attenuates motor deficit in vanadium-induced parkinsonism mice”. Anatomy and Cell Biology 2 (2020): 183-193.
  12. Ahmed F., et al.“Traditional uses and pharmacological potential of Ficus exasperata vahl”. Systematic Reviews in Pharmacy 3 (2012): 15-23.
  13. Sofowora A. “The state of Medicinal plants Research in Nigeria”. University Press, Ibadan, Nigeria, (2006): 8.
  14. Iqbal SM. “Nephroprotective Potential of a Standardized Extract of Bambusa arundinaceaIn Vitroand In Vivo Studies”. ACS Omega 21 (2022): 18159-18167.
  15. Kofie W., et al.“Phytochemical Properties of Extracts and Isolated Fractions of Leaves and Stem Bark of Ficus exasperate”. World Journal of Pharmaceutical Sciences 12 (2015): 91-101.
  16. Ayinde BA., et al.“Pharmacognosy and hypotensive evaluation of Ficus exasperate Vahl (Moraceae) leaf”. Acta Poloniae Pharmaceutica 64 (2007): 543-546.
  17. Odutuga AA., et al.“Hepatoprotective activity of ethanol extracts of Ficus exasperata leaves on acetaminophen-induced hepatotoxic rats”. Merit Research Journals2 (2004) :028-033.
  18. Ogunleye DS., et al.“Hypoglycaemic activities of the stem bark of Cola acuminata Vahl and leaf of Ficus exasperata. (P, Beauv) Schott and Endl”. Nigerian Quarterly Journal of Hospital Medicine 1 (2003): 58-60.
  19. Ijeh II and Ukweni AI. “Acute effect of administration of ethanol extracts of Ficus exasperate vahl on kidney function in albino wistar rats”. Journal of Medicinal Plants Research
    2 (2000): 027-029.
  20. Perazella MA. “Drug induced nephrotoxicity”. Expert Opinion on Drug Safety 4 (2005): 689-706.
  21. Nidhal AK., et al.“Nephro-Protective Effect of Punica granatum in Gentamicin- Induced Nephrotoxicity in Rats”. Medical Journal of Babylon (2012): 9.
  22. Janjua A., et al.“Protective effect of metformin against gentamicin induced nephrotoxicity in rabbits”. Pakistan Journal of Pharmaceutical Sciences 6 (2014): 1863-1872.
  23. Adewole SO., et al.“Effects of ficus exasperata leaf aqueous extract on renal functions of streptozotoxicin treated rats”. Folia Morphologica 1 (2012): 1-9.
  24. Isnard BC., et al.“Herbs and kidney”. American Journal of Kidney Diseases 1 (2000): 11.
  25. Hala ZE Mohamed and Merry BK Shenouda. “Amelioration of renal cortex histological alterations by aqueous garlic extract in gentamicin induced renal toxicity in albino rats: a histological and immunohistochemical study”. Alexandria Journal of Medicine1 (2021): 28-37.
  26. Mishra P., et al.“Nephroprotective role of diosgenin in gentamicin-induced renal toxicity: biochemical, antioxidant, immunological and histopathological approach”. Future Journal of Pharmaceutical Sciences 7 (2021): 169.

Citation

Citation: Oviosun Augustine., et al. “Nephroprotective Properties of Aqueous Extract of Ficus exasperata on Gentamicin-Induced Nephrotoxicity".Acta Scientific Anatomy 2.4 (2023): 12-18.

Copyright

Copyright: © 2023 Oviosun Augustine., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



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