Mosmi Medpilwar, Darshil Maru, Madhavi Vernekar and Mugdha Harmalkar*
School of Biotechnology and Bioinformatics, D.Y Patil Deemed to be University, Navi Mumbai, Maharashtra, India
*Corresponding Author: Mugdha Harmalkar, School of Biotechnology and Bioinformatics, D.Y Patil Deemed to be University, Navi Mumbai, Maharashtra, India.
Received: November 28, 2019; Published: December 11, 2019
The present study aims to evaluate the antimicrobial and anticancer activity of ethanolic extracts of Bougainvillea shubhra and Bougainvillea peruviana. The ethanolic extracts of both the plants showed antimicrobial activity against gram positive bacteria, gram negative bacteria and yeast. The antifungal activity of the extracts was measured as extent of mycelial growth and sporulation. By 96 h of incubation, the mycelial growth in control plates showed 3.8 fold increase and extensive sporulation whereas in plates pre-coated with ethanolic extracts of B. shubhra and B. peruviana, the fold increase in mycelial growth was 2.9 and 2.7 fold respectively with restricted mycelial sporulation. To study the anticancer activity of ethanolic extracts, Hela cells were treated with 20, 60 and 100 μg/ml of extracts. No inhibitory effects of the extracts were evident till 24 h incubation. After 48 h of treatment, 20 and 60 μg/ml of extracts showed no distinct changes in cells morphology, however at 100 μg/ml dose the cell number diminished, morphology was significantly altered and few floating (non-adherent) cells suggesting apoptotic cell population was observed. The cell viability measured by trypan blue exclusion method showed considerable decrease in viable cells at 100 μg/ml concentration of extracts. MTT assay showed a dose dependent inhibition of proliferation of the Hela cells. At 100 µg/ml concentration, the ethanolic extracts of B. shubra and B. peruviana showed 65% and 54% of growth inhibition respectively. These results suggest that both B. shubra and B. peruviana posses anticancer activity.
Keywords: Antibacterial; Antifungal; Anticancer; Bougainvillea Shubhra; Bougainvillea peruviana
Citation: Mugdha Harmalkar., et al. “Evaluation of Anti-Microbial and Anti-Cancer Activity of Ethanolic Extracts of Bougainvillea shubhra and Bougainvillea peruviana".Acta Scientific Nutritional Health 4.1 (2020): 75-79.
Copyright: © 2020 Mugdha Harmalkar., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.