Abstract

  Though the genetic susceptibility to T2D is polygenic, with almost 50 loci identified to be associated with T2D risk, single nucleotide polymorphisms (SNP) at the transcription factor 7-like 2 gene (TCF7L2) have been strongly and consistently associated with T2D risk in various populations and ethnic groups. The exact mechanism by which TCF7L2 variations predispose to T2D is not clear. TCF7L2, which spans a 215 863 base-pair region on chromosome 10q25.3, encodes the transcription factor TCF7L2 that is involved in the Wnt signaling pathway, which seems to be critical to pancreatic islet development and adipogenesis through the dysregulation of proglucagon gene expression. Type 2 diabetes is reported to be associated with a systemic low-grade inflammation as indicated by increased levels of circulating acute-phase proteins like CRP and of IL-6. Scientific researchers believe that through exploiting the recent genomic information and better understanding of nutrient-gene interactions, better health outcomes can be achieved if nutritional requirements are customized for each individual taking into consideration the inherited genetic characteristics depending on life stage, dietary preferences and health status.

Subjects: Cases group: 25 patients diagnosed with Type II Diabetes Mellitus selected from the outpatient clinics of the National Nutrition Institute and Ain Shams University Hospitals. Controls group: 25 age and sex matched, apparently healthy patients’ relatives, with no known history of Diabetes.

Methods: Full demographic and clinical data will be collected from patients’ data base. Then all individuals included in this study were subjected to a peripheral whole blood on EDTA and serum samples will be obtained and the following laboratory investigations will be performed: 1-Random blood glucose. 2-Glycated Hemoglobin (HbA1C). 3- Genotyping of TCF7L2 gene polymorphism by Real time PCR (Polymerase Chain Reaction). 4-Interleukin 6 (IL6) Assay by ELISA.

Results: There was a significant positive correlation between levels of serum IL-6 and dietary fat intake in the case group; however, the same correlation was not statistically significant with caloric or carbohydrate intakes. There was no significant correlations between IL-6 and either caloric intake, carbohydrate intake, or fat intake in the control group. No significant correlation between TCF7L2 genotypes and the levels of IL-6 in the in the cases group. Correlation between levels of IL-6 and the cycle threshold (ct) of the risk (T) allele (indicating allele expression) shows no statistical significance in cases or control group.

Keywords: Type 2 Diabetes; TCF7L2; Cycle Threshold

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Citation

Citation: Mahmoud Ibrahim Mohamed Hassanein Elghazawy., et al. “TCF7L2 Polymorphism and Inflammation in Type II Diabetes: A Nutrigenetic Pilot Study".Acta Scientific Pharmaceutical Sciences 4.1 (2020): 02-08.

Copyright

Copyright: © 2020 Mahmoud Ibrahim Mohamed Hassanein Elghazawy., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.